RESUMO
A simple method for short-term recharge of SV40-immortalized marrow stromal cell (MSC) lines based on their specific interaction with the appropriate haemopoietic cells is described. During the first week after the recharge the production of polymorphonuclear and mononuclear blood cells is an order of magnitude higher than in the recharged primary MSC cultures. Progenitor-derived granulopoiesis is predominant. The long-term repopulating potential of the haemopoietic stem cells (HSC) in the recharged cultures is demonstrated when they are cultured in tissue culture wells (Sutherland et al., 1990). The blood cells produced in the later passages show signs of malignancy.
Assuntos
Medula Óssea/fisiologia , Hematopoese/fisiologia , Células-Tronco Hematopoéticas/fisiologia , Vírus 40 dos Símios/crescimento & desenvolvimento , Animais , Medula Óssea/microbiologia , Células Cultivadas , Contagem de Leucócitos , Leucócitos Mononucleares/fisiologia , Estudos Longitudinais , Camundongos , Camundongos Endogâmicos BALB C , Neutrófilos/fisiologiaRESUMO
The combined protective effect of a novel naturally glycosylated Cu/Zn-containing superoxide dismutase, produced by the fungus Humicula lutea (HL-SOD) strain 103, and the selective anti-influenza drug rimantadine hydrochloride (Rim) was evaluated in experimental virus infection in mice, induced with influenza virus A/Aichi/2/68 (H3N2). A combined application of HL-SOD and Rim in doses, which by themselves did not protect significantly mice against the infection, resulted in a synergistically increased protection, determined on the basis of protective indices. Lung virus titers, lung weights and consolidation and mortality rates were all decreased significantly, while survival times were prolonged.
Assuntos
Antivirais/uso terapêutico , Infecções por Orthomyxoviridae/prevenção & controle , Rimantadina/uso terapêutico , Superóxido Dismutase/uso terapêutico , Animais , Aprotinina/uso terapêutico , Cobre/farmacologia , Sinergismo Farmacológico , Quimioterapia Combinada , Pulmão/patologia , Camundongos , Infecções por Orthomyxoviridae/mortalidade , Infecções por Orthomyxoviridae/patologia , Rimantadina/farmacologia , Superóxido Dismutase/farmacologia , Zinco/farmacologiaAssuntos
Aciclovir/farmacologia , Antibióticos Antineoplásicos/farmacologia , Antimetabólitos/farmacologia , Herpesvirus Humano 1/efeitos dos fármacos , Herpesvirus Humano 2/efeitos dos fármacos , Ácido Micofenólico/farmacologia , Efeito Citopatogênico Viral/efeitos dos fármacos , Sinergismo Farmacológico , Humanos , Replicação Viral/efeitos dos fármacosRESUMO
The presence of particular cells, discussed in literature as disintegrated parenchymal hepatic cells makes impression during a cytological study. We examined 21 patients with chronic active hepatitis. They had the above described cells found in the bile sediment by means of light microscope. The sediments were studied with electronic microscope as well. Ultrastructural proofs of the fact that these cells originate from hepatic parenchyma were found in 3 of the cases. The most important evidences are: the structure of the granular endoplasmic riticulum and of the mitochondria, as well as the precipitation of microcorpuscles.