A pioneer morphological and genetic study of the intertidal fauna of the Gerlache Strait (Antarctic Peninsula).

The underexplored intertidal ecosystems of Antarctica are facing rapid changes in important environmental factors. Associated with temperature increase, reduction in coastal ice will soon expose new ice-free areas that will be colonized by local or distant biota. To enable detection of future changes in faunal composition, a biodiversity baseline is urgently required. Here, we evaluated intertidal faunal diversity at 13 locations around the Gerlache Strait (western Antarctic Peninsula), using a combination of a quadrat approach, morphological identification and genetic characterization. Our data highlight a community structure comprising four generally distributed and highly abundant species (the flatworm Obrimoposthia wandeli, the bivalve Kidderia subquadrata, and the gastropods Laevilitorina umbilicata and Laevilitorina caliginosa) as well as 79 rarer and less widely encountered species. The most abundant species thrive in the intertidal zone due to their ability to either survive overwinter in situ or to rapidly colonize this zone when conditions allow. In addition, we confirmed the presence of multiple trophic levels at nearly all locations, suggesting that complex inter-specific interactions occur within these communities. Diversity indices contrasted between sampling locations (from 3 to 32 species) and multivariate approaches identified three main groups. This confirms the importance of environmental heterogeneity in shaping diversity patterns within the investigated area. Finally, we provide the first genetic and photographic baseline of the Antarctic intertidal fauna (106 sequences, 137 macrophotographs), as well as preliminary insights on the biogeography of several species. Taken together, these results provide a timely catalyst to assess the diversity and to inform studies of the potential resilience of these intertidal communities.

Laevilacunaria (Mollusca, Gastropoda) in the Southern Ocean: A comprehensive occurrence dataset.

Background: The present dataset is a compilation of georeferenced occurrences of the littorinid genus Laevilacunaria Powell, 1951 (Mollusca, Gastropoda) in the Southern Ocean. Occurrence data were obtained from field expeditions (Antarctic and sub-Antarctic sampling) between 2015 and 2022, together with a review of published literature including records from 1887 to 2022. Three Laevilacunaria species have been recorded from the Southern Ocean: Laevilacunariabennetti, L.antarctica and L.pumilio. New information: The present dataset includes 75 occurrences, representing the most exhaustive database of this Antarctic and sub-Antarctic littorinid genus. The publication of this data paper was funded by the Belgian Science Policy Office (BELSPO, contract n°FR/36/AN1/AntaBIS) in the Framework of EU-Lifewatch as a contribution to the SCAR Antarctic biodiversity portal (biodiversity.aq).

Revealing the hidden biodiversity of Antarctic and the Magellanic Sub-Antarctic Ecoregion: A comprehensive study of aquatic invertebrates from the BASE Project.

Background: Antarctica, its outlying archipelagoes and the Magellanic Subantarctic (MSA) ecoregion are amongst the last true wilderness areas remaining on the planet. Therefore, the publication, citation and peer review of their biodiversity data are essential. The new Millennium Institute Biodiversity of Antarctic and Subantarctic Ecosystems (BASE), a Chilean scientific initiative funded by the National Agency of Research and Innovation, contributes 770 new records of aquatic invertebrates as a point of reference for present-day biodiversity research at these latitudes. New information: The occurrence dataset presented here has never been released before and is the result of the systematic recording of occurrences of several taxa across the Antarctic, Subantarctic and Magellanic Subantarctic ecoregions. We collected data from marine and freshwater invertebrates across numerous samplings from 2008 to 2023. From the 770 occurrences, we identified 160 taxa, 125 at species level and 35 at the genus level. The database has been registered in the Global Biodiversity Information Facility (GBIF). The publication of this data paper was funded by the Belgian Science Policy Office (BELSPO, contract n°FR/36/AN1/AntaBIS) in the Framework of EU-Lifewatch as a contribution to the SCAR Antarctic biodiversity portal (biodiversity.aq).

Complete distribution of the genus Laevilitorina (Littorinimorpha, Littorinidae) in the Southern Hemisphere: remarks and natural history.

Littorinid snails are present in most coastal areas globally, playing a significant role in the ecology of intertidal communities. Laevilitorina is a marine gastropod genus distributed exclusively in the Southern Hemisphere, with 21 species reported from South America, the sub-Antarctic islands, Antarctica, New Zealand, Australia and Tasmania. Here, an updated database of 21 species generated from a combination of sources is presented: 1) new field sampling data; 2) published records; 3) the Global Biodiversity Information Facility (GBIF) and The Atlas of Living Australia (ALA), to provide a comprehensive description of the known geographic distribution of the genus and detailed occurrences for each of the 21 species. The database includes 813 records (occurrences), 53 from field sampling, 174 from the literature, 128 from GBIF, and 458 from ALA. West Antarctica had the highest species richness (8 species), followed by sub-Antarctic islands of New Zealand (4 species) and the south-east shelf of Australia (4 species). The provinces of Magellan, New Zealand South Island, and sub-Antarctic Islands of the Indian Ocean include two species each. This study specifically highlights reports of L.pygmaea and L.venusta, species that have been almost unrecorded since their description. Recent advances in molecular studies of L.caliginosa showed that this species does not correspond to a widely distributed taxon, but to multiple divergent lineages distributed throughout the Southern Ocean. Ongoing molecular and taxonomic studies are necessary for a better understanding of the diversity and biogeography of this genus.

Phylogeography and genetic diversity of the microbivalve Kidderia subquadrata, reveals new data from West Antarctic Peninsula.

It is well established that Antarctic biodiversity has been strongly influenced by rapid climatic fluctuations during the Quaternary. Marine invertebrates from Antarctica constitute an interesting lens through which to study the impacts of the last glacial periods as glaciation impacted the distribution and intraspecific genetic variation of these animals. However, the impact on the spatial genetic distribution and historical demography of local processes in areas adjacent to the West Antarctic Peninsula (WAP) is less clear. Here we present new genetic information on the bivalve Kidderia subquadrata, a small mollusk that inhabits intertidal rocky island ecosystems throughout the WAP. Using a phylogeographical approach, we examined the spatial patterns of genetic diversity in this brooder species to test the hypothesis of strong genetic structure in incubating organisms and the hypothesis of glacial refugia in organisms with limited dispersion. We found evidence of strong genetic structure among populations of the WAP and a recent expansion in the South Shetland Islands. Our findings are concordant with the predictions that incubating organisms, abundant in Antarctica, present a strong genetic structure among their populations and also support the hypothesis of glacial refugia in organisms with limited dispersion. The effect of the coastal current pattern in the WAP is suggested as a driver to the local spatial dynamics of the genetic diversity distribution. Although genetic information about this microbivalve is still scarce, the knowledge reported here has increased our understanding of the evolutionary patterns of this organism that is endemic to the Southern Ocean.

Divergence time and species delimitation of microbivalves in the Southern Ocean: the case of Kidderia species.

The systematics of Subantarctic and Antarctic near-shore marine benthic invertebrates requires major revision and highlights the necessity to incorporate additional sources of information in the specimen identification chart in the Southern Ocean (SO). In this study, we aim to improve our understanding of the biodiversity of Kidderia (Dall 1876) through molecular and morphological comparisons of Antarctic and Subantarctic taxa. The microbivalves of the genus Kidderia are small brooding organisms that inhabit intertidal and shallow subtidal rocky ecosystems. This genus represents an interesting model to test the vicariance and dispersal hypothesis in the biogeography of the SO. However, the description of Kidderia species relies on a few morphological characters and biogeographic records that raise questions about the true diversity in the group. Here we will define the specimens collected with genetic tools, delimiting their respective boundaries across provinces of the SO, validating the presence of two species of Kidderia. Through the revision of taxonomic issues and species delimitation, it was possible to report that the Antarctic species is Kidderia subquadrata and the species recorded in the Subantarctic islands Diego Ramirez, South Georgia and the Kerguelen Archipelago is Kidderia minuta. The divergence time estimation suggests the origin and diversification of Kidderia lineages are related to historical vicariant processes probably associated with the separation of the continental landmasses close to the late Eocene. Supplementary Information: The online version contains supplementary material available at 10.1007/s00300-021-02885-6.

Unveiling the unknown phylogenetic position of the scallop Austrochlamys natans and its implications for marine stewardship in the Magallanes Province.

Two species of scallop, Austrochlamys natans ("Ostión del Sur") and Zygochlamys patagonica ("Ostión patagonico") are presently exploited in the southern part of the Magallanes Province (MP). The lack of clarity in taxonomic identification and ecological aspects is generating both erroneous extraction statistics and an unperceived harvesting pressure on A. natans and Z. patagonica. We aim to discriminate these Magallanes scallops accurately, improve our understanding of their complex natural history and discuss possible implications for their management and conservation status, given the current fisheries statistics. To achieve these goals, we present a complete review of the historical identification of the Magallanes scallop and a multi-locus molecular phylogeny which allowed us to recover the phylogenetic position of A. natans. We sampled 54 individuals from five localities across the southern Pacific coast of the MP. We calculated the depth of the byssal notch (BND) and shell height (VH) ratio from morphological characters and conducted phylogenetic reconstructions with mitochondrial (12S and 16S) and nuclear markers (28S) using Bayesian and maximum likelihood analyses. Both morphology and molecular phylogeny identified two distinct entities, Z. patagonica and a distinct, highly divergent lineage that corresponds to A. natans. Our study provides integrative evidence to alert the current fishery management and the need for further conservation studies.

Diversity of benthic marine mollusks of the Strait of Magellan, Chile (Polyplacophora, Gastropoda, Bivalvia): a historical review of natural history.

An increase in richness of benthic marine mollusks towards high latitudes has been described on the Pacific coast of Chile in recent decades. This considerable increase in diversity occurs specifically at the beginning of the Magellanic Biogeographic Province. Within this province lies the Strait of Magellan, considered the most important channel because it connects the South Pacific and Atlantic Oceans. These characteristics make it an interesting area for marine research; thus, the Strait of Magellan has historically been the area with the greatest research effort within the province. However, despite efforts there is no comprehensive and updated list of the diversity of mollusks within the Strait of Magellan up to now. This study consisted of a complete bibliographic review of all available literature that included samples of mollusks in the Strait of Magellan. More than 300 articles were reviewed, covering 200 years of scientific knowledge. There were 2579 records belonging to 412 taxa, of which 347 are valid species. Of the total valid species, 44 (~13%) are considered of doubtful presence in the Strait. This work increases the known richness of mollusks of the Strait of Magellan by 228%; it is also the first report that integrates all available diversity studies of the three most speciose classes of benthic mollusks (Gastropoda, Bivalvia and Polyplacophora) from the Strait of Magellan.

The Pros and Cons of Using Algal Polysaccharides as Prebiotics.

Macroalgae stand out for their high content of dietary fiber (30-75%) that include soluble, sulfated (fucoidan, agaran, carrageenan, and ulvan) and non-sulfated (laminaran and alginate) polysaccharides. Many studies indicate that these compounds exert varied biological activities and health-promoting effects and for this reason, there is a growing interest for using them in food products. The aim of this review was to critically evaluate prebiotic properties of algal polysaccharides, i.e., their ability to exert biological activities by modulating the composition and/or diversity of gut microbiota (GM). Pre-clinical studies show that the non-sulfated alginate and laminaran are well-fermented by GM, promoting the formation of short chain fatty acids (SCFAs) including butyrate, and preventing that of harmful putrefactive compounds (NH3, phenol, p-cresol, indole and H2S). Alginate increases Bacteroides, Bifidobacterium, and Lactobacillus species while laminaran mostly stimulates Bacteroides sp. Results with sulfated polysaccharides are more questionable. Agarans are poorly fermentable but agarose-oligosaccharides exhibit an interesting prebiotic potential, increasing butyrate-producing bacteria and SCFAs. Though carrageenan-oligosaccharides are also fermented, their use is currently limited due to safety concerns. Regarding fucoidan, only one study reports SCFAs production, suggesting that it is poorly fermented. Its effect on GM does not indicate a clear pattern, making difficult to conclude whether it is beneficial or not. Notably, fucoidan impact on H2S production has not been evaluated, though some studies report it increases sulfate-reducing bacteria. Ulvan is badly fermented by GM and some studies show that part of its sulfate is dissimilated to H2S, which could affect colonic mitochondrial function. Accordingly, these results support the use of laminaran, alginate and agaro-oligosaccharides as prebiotics while more studies are necessary regarding that of fucoidan, carrageenan and ulvan. However, the realization of clinical trials is necessary to confirm such prebiotic properties in humans.

Distribution of the genus Boeckella (Crustacea, Copepoda, Calanoida, Centropagidae) at high latitudes in South America and the main Antarctic biogeographic regions.

Copepods are present in numerous aquatic environments, playing key roles in food webs, and are thought to be useful indicators of environmental change. Boeckella is a calanoid copepod genus distributed mainly in the Southern Hemisphere, with 14 species reported at higher southern latitudes in South America and Antarctica. We present an updated database of these 14 species of Boeckella generated from a combination of three sources: 1) new field sampling data, 2) published records, and 3) Global Biodiversity Information Facility (GBIF), to provide a comprehensive description of the geographic distribution of the genus south of latitude 40°S in southern South America and the three main terrestrial biogeographic regions of Antarctica. The database includes 380 records, 62 from field sampling, 278 from the literature and 40 from GBIF. Southern South America, including the Falkland/Malvinas Islands, had the highest species richness and number of records (14 and 297, respectively), followed by the sub-Antarctic islands (5 and 34), South Orkney Islands (2 and 14), South Shetland Islands (1 and 23), Antarctic Peninsula (1 and 10) and finally continental Antarctica (1 and 2). Boeckellapoppei Mrázek, 1901 is the only representative of the genus, and more widely the only terrestrial/freshwater invertebrate, currently reported from all three main biogeographic regions in Antarctica (sub-Antarctic islands, maritime and continental Antarctic). Future development of molecular systematic studies in this group should contribute to assessing the correspondence between morphological taxonomy and molecular evolutionary radiation.

A new record of kelp Lessonia spicata (Suhr) Santelices in the Sub-Antarctic Channels: implications for the conservation of the "huiro negro" in the Chilean coast.

The Katalalixar National Reserve (KNR) lies in an isolated marine protected area of Magellan Sub-Antarctic channels, which represent an important area for marine biodiversity and macroalgal conservation. The present study is the first report of the species Lessonia spicata, "huiro negro", in the Magellan Sub-Antarctic channels. This finding has implications for macroalgal biogeography and conservation concerns in the Chilean coast. In the ecological assessments of the KNR in 2018 we found populations of L. spicata, specifically on rocky shores of Torpedo Island and Castillo Channel. The morphological identification and molecular phylogeny based on nuclear (ITS1) sequences revealed that these populations of Lessonia are within the lineage of L. spicata of central Chile. This report increases the species richness of kelps for the Magellan Sub-Antarctic Channels from two to three confirmed species (L. flavicans, L. searlesiana and L. spicata), and it also extends the southern distribution range of L. spicata. This species has high harvest demand and is moving towards southern Chile; thus, these populations should be considered as essential for macroalgal conservation in high latitudes of South America.

Next-generation sequencing yields the complete organellar genomes of kelp Lessonia flavicans (Lessoniaceae, Phaeophyceae) from the Sub-Antarctic ecoregion of Magallanes, Chile.

The generitype Lessonia flavicans Bory is an endemic and important kelp from Sub-Antarctic Magellanic ecoregion that shows affinity to extreme salinity, temperature, and photoperiod conditions. Genomic analysis of L. flavicans from Rinconada Bulnes, Punta Arenas, Chile, resulted in the assembly of its organellar genomes. The L. flavicans complete mitogenome is 37,226 base pairs (bp) in length and contains 66 genes (GenBank accession number MN561186), the complete plastid genome is 130,085 bp and has 173 genes (MN561187) and the data assembled 8205 bp of the nuclear ribosomal cistron (MN561188). The organellar genomes are similar in structure and content to L. spicata (Suhr) Santelices and other Laminariales.

Trophic ecology of two co-existing Sub-Antarctic limpets of the genus Nacella: spatio-temporal variation in food availability and diet composition of Nacella magellanica and N. deaurata.

Interactions between algae and herbivores can be affected by various factors, such as seasonality and habitat structure. Among herbivores inhabiting marine systems, species of the order Patellogastropoda are considered key organisms in many rocky coasts of the world. Nacella species are one of the most dominant macro-herbivores on the rocky shores of the sub-Antarctic ecoregion of Magellan. However, the importance of its key role must be associated with its trophic ecology. The objective of this work was to evaluate spatial and temporal variabilities in the dietary composition of two intertidal Nacella species, considering grazing on macro- (macroalgae) and microscopic (periphyton) food. The composition of periphyton and the availability of macroalgae in the winter and summer seasons were examined at two localities of the Magellanic province, alongside the gut contents of N. magellanica and N. deaurata. The dietary composition differed between the two Nacella species, as well as between seasons and locations. The differences observed in the diet of the two species of Nacella may be mainly due to their respective distributions in the intertidal zone. Both species presented a generalist strategy of grazing, which is relationed to the seasonality of micro- and macroalgae availability and to the variability of the assemblages between the localities. This research was the first to perform a detailed study of the diet of intertidal Nacella species.

Genetics, Gene Flow, and Glaciation: The Case of the South American Limpet Nacella mytilina.

Glacial episodes of the Quaternary, and particularly the Last Glacial Maximum (LGM) drastically altered the distribution of the Southern-Hemisphere biota, principally at higher latitudes. The irregular coastline of Patagonia expanding for more than 84.000 km constitutes a remarkable area to evaluate the effect of Quaternary landscape and seascape shifts over the demography of near-shore marine benthic organisms. Few studies describing the biogeographic responses of marine species to the LGM have been conducted in Patagonia, but existing data from coastal marine species have demonstrated marked genetic signatures of post-LGM recolonization and expansion. The kelp-dweller limpet Nacella mytilina is broadly distributed along the southern tip of South America and at the Falkland/Malvinas Islands. Considering its distribution, abundance, and narrow bathymetry, N. mytilina represents an appropriate model to infer how historical and contemporary processes affected the distribution of intraspecific genetic diversity and structure along the southern tip of South America. At the same time, it will be possible to determine how life history traits and the ecology of the species are responsible for the current pattern of gene flow and connectivity across the study area. We conducted phylogeographic and demographic inference analyses in N. mytilina from 12 localities along Pacific Patagonia (PP) and one population from the Falkland/Malvinas Islands (FI). Analyses of the mitochondrial gene COI in 300 individuals of N. mytilina revealed low levels of genetic polymorphism and the absence of genetic differentiation along PP. In contrast, FI showed a strong and significant differentiation from Pacific Patagonian populations. Higher levels of genetic diversity were also recorded in the FI population, together with a more expanded genealogy supporting the hypothesis of glacial persistence of the species in these islands. Haplotype genealogy, and mismatch analyses in the FI population recognized an older and more complex demographic history than in PP. Demographic reconstructions along PP suggest a post-LGM expansion process (7.5 ka), also supported by neutrality tests, mismatch distribution and maximum parsimony haplotype genealogies. Migration rate estimations showed evidence of asymmetrical gene flow from PP to FI. The absence of genetic differentiation, the presence of a single dominant haplotype, high estimated migration rates, and marked signal of recent demographic growth, support the hypothesis of rapid post-glacial expansion in N. mytilina along PP. This expansion could have been sustained by larval and rafting-mediated dispersal of adults from northernmost populations following the Cape Horn Current System. Marked genetic differentiation between PP and FI could be explained through differences in their respective glacial histories. During the LGM, Pacific Patagonia (PP) was almost fully covered by the Patagonian Ice Sheet, while sheet coverage in the FI ice was restricted to small cirques and valleys. As previously recorded in the sister-species N. magellanica, the FI rather than represent a classical glacial refugium for N. mytilina, seems to represent a sink area and/or a secondary contact zone. Accordingly, historical and contemporary processes, contrasting glacial histories between the analyzed sectors, as well as life history traits constitute the main factors explaining the current biogeographical patterns of most shallow Patagonian marine benthic organisms.

Richness, systematics, and distribution of molluscs associated with the macroalga Gigartina skottsbergii in the Strait of Magellan, Chile: A biogeographic affinity study.

Knowledge about the marine malacofauna in the Magellan Region has been gained from many scientific expeditions that were carried out during the 19th century. However, despite the information that exists about molluscs in the Magellan Region, there is a lack of studies about assemblages of molluscs co-occurring with macroalgae, especially commercially exploitable algae such as Gigartina skottsbergii, a species that currently represents the largest portion of carrageenans within the Chilean industry. The objective of this study is to inform about the richness, systematics, and distribution of the species of molluscs associated with natural beds in the Strait of Magellan. A total of 120 samples from quadrates of 0.25 m(2) were obtained by SCUBA diving at two sites within the Strait of Magellan. Sampling occurred seasonally between autumn 2010 and summer 2011: 15 quadrates were collected at each site and season. A total of 852 individuals, corresponding to 42 species of molluscs belonging to Polyplacophora (9 species), Gastropoda (24), and Bivalvia (9), were identified. The species richness recorded represents a value above the average richness of those reported in studies carried out in the last 40 years in sublittoral bottoms of the Strait of Magellan. The biogeographic affinity indicates that the majority of those species (38%) present an endemic Magellanic distribution, while the rest have a wide distribution in the Magellanic-Pacific, Magellanic-Atlantic, and Magellanic-Southern Ocean. The molluscs from the Magellan Region serve as study models for biogeographic relationships that can explain long-reaching patterns and are meaningful in evaluating possible ecosystemic changes generated by natural causes or related to human activities.