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Exp Parasitol ; 137: 66-73, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24378477

RESUMO

The experimental system of Taenia crassiceps cysticerci infection in BALB/c mice is considered to be the most representative model of cysticercosis. In our work, mice were sacrificed 7 and 30days after infection, and pouch fluid was collected to determine the number of accumulated cells and the concentrations of IFNγ, IL-2, IL-4, IL-6, IL-10 and nitric oxide. The injection of 50 nonbudding cysticerci into normal mouse dorsal air pouches induced a high level of IFNγ and nitric oxide production relative to the parasite load. The air pouch provides a convenient cavity that allows studying the cellular immunological aspects of the T. crassiceps parasite. The nonbudding cysticerci recovered from the air pouches contained cells that can reconstitute complete cysts in the peritoneal cavity of mice. In conclusion, these results demonstrate that the air pouch model is an alternative tool for the evaluation of the immune characteristics of T. crassiceps infection.


Assuntos
Modelos Animais de Doenças , Taenia/imunologia , Teníase/imunologia , Animais , Cultura em Câmaras de Difusão , Exsudatos e Transudatos/citologia , Exsudatos e Transudatos/imunologia , Feminino , Interferon gama/análise , Interleucinas/análise , Camundongos , Camundongos Endogâmicos BALB C , Óxido Nítrico/metabolismo , Cavidade Peritoneal/parasitologia
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