Revelations on photosystem II, thermoluminescence, and artificial photosynthesis: a retrospective of Govindjee from fundamentals to applications.

Photosynthesis, as one of the most important chemical reactions, has powered our planet for over four billion years on a massive scale. This review summarizes and highlights the major contributions of Govindjee from fundamentals to applications in photosynthesis. His research included primary photochemistry measurements, in the picosecond time scale, in both Photosystem I and II and electron transport leading to NADP reduction, using two light reactions. He was the first to suggest the existence of P680, the reaction center of PSII, and to prove that it was not an artefact of Chlorophyll a fluorescence. For most photobiologists, Govindjee is best known for successfully exploiting Chlorophyll a fluorescence to understand the various steps in photosynthesis as well as to predict plant productivity. His contribution in resolving the controversy on minimum number of quanta in favor of 8-12 vs 3-4, needed for the evolution of one molecule of oxygen, is a milestone in the area of photosynthesis research. Furthermore, together with Don DeVault, he is the first to provide the correct theory of thermoluminescence in photosynthetic systems. His research productivity is very high: ~ 600 published articles and total citations above 27,000 with an h-index of 82. He is a recipient of numerous awards and honors including a 2022: Lifetime Achievement Award of the International Society of Photosynthesis Research. We hope that the retrospective of Govindjee described in this work will inspire and stimulate the readers to continue probing the photosynthetic apparatuses with new discoveries and breakthroughs.

Cotyledonary leaves effectively shield the true leaves in Ricinus communis L. from copper toxicity.

The stress-buffering effects of cotyledonary leaves of Ricinus communis and the protection thus offered to the true leaves upon exposure to copper stress was performed by analyzing bioaccumulation of Cu and associated metabolic processes in the presence and absence of cotyledonary leaves. One-month-old seedlings of R. communis were treated with various concentrations of CuSO4 for 6 d under hydroponics with quarter strength modified Hoagland medium. Even though the photosynthetic pigments showed a decreasing trend with an increase in CuSO4 concentration and days of exposure in cotyledonary and true leaves, it was significant in true leaves with excised cotyledonary leaves. The results of chlorophyll a fluorescence parameters indicated that toxic levels of CuSO4 do not impart any major negative effect on the photochemistry of true leaves along with cotyledonary leaves. The analysis of osmolality, malondialdehyde, and metabolites showed a significantly increasing trend in true leaves with excised cotyledonary leaves. The above observations were strongly supported by higher Cu bioaccumulation in true leaves with excised cotyledonary leaves. The results suggest that the cotyledonary leaves have got the potential to evade Cu toxicity and thereby R. communis can be effectively used for the phytoremediation of Cu contaminated lands.

Plant response to heavy metal stress toxicity: the role of metabolomics and other omics tools.

Metabolomic investigations offers a significant foundation for improved comprehension of the adaptability of plants to reconfigure the key metabolic pathways and their response to changing climatic conditions. Their application to ecophysiology and ecotoxicology help to assess potential risks caused by the contaminants, their modes of action and the elucidation of metabolic pathways associated with stress responses. Heavy metal stress is one of the most significant environmental hazards affecting the physiological and biochemical processes in plants. Metabolomic tools have been widely utilised in the massive characterisation of the molecular structure of plants at various stages for understanding the diverse aspects of the cellular functioning underlying heavy metal stress-responsive mechanisms. This review emphasises on the recent progressions in metabolomics in plants subjected to heavy metal stresses. Also, it discusses the possibility of facilitating effective management strategies concerning metabolites for mitigating the negative impacts of heavy metal contaminants on the growth and productivity of plants.