Experimental validation of specialized questioning techniques in conservation.

Conservation increasingly relies on social science tools to understand human behavior. Specialized questioning techniques (SQTs) are a suite of methods designed to reduce bias in social surveys and are widely used to collect data on sensitive topics, including compliance with conservation rules. Most SQTs have been developed in Western, industrialized, educated, rich, and democratic countries, meaning their suitability in other contexts may be limited. Whether these techniques perform better than conventional direct questioning is important for those considering their use. We designed an experiment to validate the performance of four SQTs (unmatched count technique, randomized response technique, crosswise model, and bean method) against direct questions when asking about a commonly researched sensitive behavior in conservation, wildlife hunting. We developed fictional characters, and for each method asked respondents to report the answers that each fictional character should give when asked if they hunt wildlife. We collected data from 609 individuals living close to protected areas in two different cultural and socioeconomic contexts (Indonesia and Tanzania) to quantify the extent to which respondents understood and followed SQT instructions and to explore the sociodemographic factors that influenced a correct response. Data were modeled using binomial general linear mixed models. Participants were more likely to refuse to answer questions asked using SQTs compared with direct questions. Model results suggested that SQTs were harder for participants to understand. Demographic factors (e.g., age and education level) significantly influenced response accuracy. When sensitive responses to sensitive questions were required, all SQTs (excluding the bean method) outperformed direct questions, demonstrating that SQTs can successfully reduce sensitivity bias. However, when reviewing each method, most respondents (59-89%) reported they would feel uncomfortable using them to provide information on their own hunting behavior, highlighting the considerable challenge of encouraging truthful reporting on sensitive topics. Our results demonstrate the importance of assessing the suitability of social science methods prior to their implementation in conservation contexts.

Validación Experimental de las Técnicas de Cuestionamiento Especializado en la Conservación Resumen La conservación depende cada vez más de herramientas de las ciencias sociales para entender el comportamiento humano. Las técnicas de cuestionamiento especializado (TCE) son un conjunto de métodos diseñados para reducir el sesgo en los censos sociales y y se usan ampliamente para recolectar datos sobre temas sensibles, incluyendo el cumplimiento de las normas de conservación. La mayoría de las TCE se desarrollaron en el hemisferio occidental, en países industrializados, educados, ricos y democráticos, lo que significa que su que su idoneidad en otros contextos puede ser limitada. Quienes piensan en usar estas técnicas consideran importante saber si tienen un mejor desempeño que el cuestionamiento directo convencional. Diseñamos un experimento para validar el desempeño de cuatro TCE (técnica de conteo sin par, técnica de respuesta aleatoria, modelo transversal, método bean) frente a las preguntas directas cuando se cuestiona sobre un comportamiento sensible de interés recurrente en la conservación: la cacería de fauna. Desarrollamos personajes ficticios y para cada método le pedimos a los respondientes que reportaran las respuestas que cada personaje ficticio debería dar al cuestionársele si cazan fauna silvestre. Recolectamos datos de 609 individuos con residencia cercana a áreas protegidas en dos contextos cultural y socioeconómicamente diferentes (Indonesia y Tanzanía) para cuantificar el grado al que los respondientes entendieron y siguieron las instrucciones de las TCE y para explorar los factores sociodemográficos que influyeron sobre las respuestas correctas. Modelamos los datos mediante modelos lineales mixtos binomiales y generales. Fue más probable que los participantes se rehusaran a responder las preguntas realizadas con las TCE en comparación con las preguntas directas. Los resultados del modelo sugirieron que las TCE eran más difíciles de entender para los participantes. Los factores demográficos (p. ej.: edad y nivel educativo) influyeron significativamente la certeza de las respuestas. Cuando se requirieron respuestas sensibles a preguntas sensibles, todas las TCE (excepto el método bean) tuvieron un mejor desempeño que las preguntas directas, lo que demuestra que las TCE pueden reducir exitosamente el sesgo por sensibilidad. Sin embargo, cuando se les preguntó sobre cada método, la mayoría de los respondientes (59-89%) reportó que se sentirían incómodos si tuvieran que usarlas para proporcionar información sobre sus propios comportamientos de cacería, lo que destaca el reto considerable que es reportar honestamente los temas sensibles. Nuestros resultados demuestran la importancia de de evaluar la idoneidad de los métodos de las ciencias sociales previo a su implementación en contextos de conservación.

A mixed methods approach for measuring topic sensitivity in conservation.

Conservationists increasingly aim to understand human behaviour to inform intervention design. However, obtaining information from people about their behaviour can be challenging, particularly if the research topic is considered sensitive. Topic sensitivity may raise methodological, ethical, political and legal concerns which, if poorly addressed, can have significant impacts on research participants, the research process, data quality and the success of conservation outcomes that are informed by research findings. While considerable effort has been invested in developing techniques for reducing bias when collecting data on sensitive topics, less attention has been focused on identifying if, and why, a topic is sensitive.We use a mixed methods approach to explore how willing people are to discuss topics that could be considered sensitive (e.g. illegal wildlife hunting). Collecting data from people living near protected areas in Indonesia (n = 362) and Tanzania (n = 345), we developed and tested a psychometric scale to measure topic sensitivity at the respondent level and conducted group exercises (free-lists and pile sorts) to gain a deeper understanding of peoples' willingness to discuss different topics.The perceived sensitivity of topics varied both within and between the two focal contexts, with more topics being perceived as sensitive in Tanzania than Indonesia. Participants' knowledge of rules, and their experiences of living alongside protected areas affected how sensitive they considered topics to be.Mixed methods approaches can provide holistic and nuanced understanding of topic sensitivity. However, recognising that in-depth studies are not always feasible to implement, we demonstrate that methods, such as our Sensitivity Index, can easily be adapted for different contexts and deployed to rapidly obtain valuable insights on topic sensitivity, to help inform conservation research and practice.

Eggshell membrane for DNA sexing of the endangered Maleo ( Macrocephalon maleo).

Background: Noninvasive DNA sampling has been applied across many avian genetic studies for a variety of purposes including conservation and management of endangered birds. However, its application in megapodes is still lacking. The previous genetic studies on megapodes used either blood or fresh tissue. Here we present the first demonstration of the use of eggshell membrane for research on endangered Maleo ( Macrocephalon maleo).  Methods: We used 24 post-hatched eggshell membranes collected from two different sites, Tambun and Tanjung Binerean, in North Sulawesi, 12 samples in each. Two different DNA extraction methods: alkaline lysis method and gSYNC TM DNA Extraction Kit were applied.  To determine the sex of Maleo, we utilized PCR-based DNA sexing using CHD genes, with the primer set 2550F/2718R.  Results: We successfully extracted all samples; the mean sample concentration was 267.5 ng/µl (range 47-510.5 ng/µl) and samples were of high purity (A260/280 ratio 1.85±0.03). All samples were used to successfully identified sexes, 9 females and 15 males.  Conclusions: Our research clearly illustrates that eggshell membranes can be used for DNA sexing and open the possibility to build noninvasive DNA collections over large spatial scales for population study of endangered birds.