RESUMO
Erythro-aggregometer is a Couette viscometer which was developed to measure aggregation parameters of red blood cells. The system is based on the analysis of the light intensity backscattered by a blood suspension. It allows to approach aggregation phenomenon in terms of kinetics, structural and rheological parameters. The measurement system designed for use with a microcomputer is suitable for both research and clinical investigations.
Assuntos
Agregação Eritrocítica , Viscosidade Sanguínea , Diabetes Mellitus Tipo 1/sangue , Equipamentos e Provisões , Hematócrito , Humanos , Cinética , Luz , Microcomputadores , Mieloma Múltiplo/sangue , Espalhamento de Radiação , TemperaturaRESUMO
A therapeutic immunoadsorption system on immobilized and anti-apolipoprotein B as a plasma cholesterol lowering procedure was optimized. Several antibodies were compared and highest adsorption capacity was obtained with goat polyclonal antibodies. Optimum quantities of antibodies to be immobilized on the gel and quantities of apo-B to be applied to columns were determined. The amount of antibodies released from immunoadsorbents can be minimized by treatment with a 0.005% glutaraldehyde solution with an acceptable reduction rate of adsorption capacity. Each phase, adsorption and desorption respectively, were well-defined and synchronized so two columns could be used in parallel in an automated procedure. In these conditions, the immunoadsorption system can efficiently, specifically and safely remove cholesterol and has to be subjected to clinical trials.
Assuntos
Anticorpos/imunologia , Apolipoproteínas B/isolamento & purificação , Animais , Anticorpos Monoclonais , Apolipoproteínas B/metabolismo , Colesterol/metabolismo , Glutaral/farmacologia , Cabras , Humanos , Hipercolesterolemia/imunologia , Imunoglobulina G/análise , Técnicas de Imunoadsorção , Ratos , Sensibilidade e EspecificidadeRESUMO
An immunoadsorption system for lowering plasma cholesterol was optimized. Several polyclonal and monoclonal antibodies were compared and the best results were obtained with goat polyclonal antibodies. The optimum quantity of antibodies to be immobilized on the gel was 5 mg/ml. Taking into account two variables, i.e., 1) that the regeneration must be as complete as possible and, 2) that immunoadsorbents must be used several times without a loss of adsorption capacity, desorption was achieved with 0.3 M glycine adjusted to pH 2.8. Antibody release from the immunoadsorbent was determined and can be minimized by glutaraldehyde treatment of the immunoadsorbent. Each phase, adsorption and desorption, respectively, was well-defined and synchronized, so that two columns could be used in parallel in an automated procedure. The kinetics of plasma protein removal demonstrated the efficiency and the specificity of the procedure.