Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Mais filtros

Base de dados
Tipo de documento
País de afiliação
Intervalo de ano de publicação
1.
Eur Biophys J ; 51(1): 29-40, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35031815

RESUMO

Antimicrobial peptides are molecules synthesized by living organisms as the first line of defense against bacteria, fungi, parasites, or viruses. Since their biological activity is based on destabilization of the microbial membranes, a study of direct interaction forces between antimicrobial peptides and biomimetic membranes is very important for understanding the molecular mechanisms of their action. Herein, we use atomic force spectroscopy to probe the interaction between atomic force microscopy (AFM) tips functionalized with magainin 1 and supported lipid bilayers (SLBs) mimicking electrically uncharged membranes of normal eukaryotic cells and negatively charged membranes of bacterial cells. The investigations performed on negatively charged SLBs showed that the magainin 1 functionalized AFM tips are quickly adsorbed into the SLBs when they approach, while they adhere strongly to the lipid membrane when retracted. On contrary, same investigations performed on neutral SLBs showed mechanical resistance of the lipid membrane to the tip breakthrough and negligible adhesion force at detachment.


Assuntos
Biomimética , Bicamadas Lipídicas , Magaininas , Microscopia de Força Atômica , Análise Espectral
2.
Langmuir ; 33(21): 5188-5196, 2017 05 30.
Artigo em Inglês | MEDLINE | ID: mdl-28485609

RESUMO

Partial wetting on the nanoscale may result in the formation of sessile liquid nanodroplets on flat substrates. In this case, the molecular forces generate a strong interaction between nanodroplet interfaces. This interaction is expressed in the mean-field approximation by the disjoining pressure and determines an important deviation from the spherical cap shape of the nanodroplets. This deviation is observed on the atomic force microscopy images of sessile nanodroplets of oleic acid on glass. The disjoining pressure was manipulated by hydroxylation of the glass surface. This surface modification generated a strong negative disjoining pressure due to structural forces arising from the orientation of oleic acid molecules with their polar heads toward the substrate. As a result, the shape of oleic acid nanodroplets showed large deviations from the spherical cap shape, with the liquid-vapor interface tilting angle with respect to the plane substrate having a maximum (herein considered to be the contact angle) a certain distance from the substrate, followed by its decrease to zero at the droplet edge. The integration of the augmented Young-Laplace equation, where the dependence of the negative structural disjoining pressure on the interface separation distance was assumed to be an exponential decay, yielded height profiles of droplets in good agreement with the experiment.

3.
Nanotechnology ; 28(25): 255302, 2017 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-28471756

RESUMO

Titanium oxide/silicon oxide (TiO2/SiO2) 2D patterns were obtained by magnetron sputtering depositions of Ti on close-packed and size-reduced colloidal masks on Si and quartz substrates, followed by mask lift-off and ending with thermal oxidation. The physical processes involved in growing 2D Ti patterns and their oxidation are analyzed. For the magnetron sputtering deposition, two regimes are considered: the low-pressure regime when the flux of sputtered atoms is anisotropic, and the high-pressure regime, when the flux of sputtered atoms is isotropic due to frequent collisions. Moreover, magnetron sputtering operation modes, such as dc sputtering and high power impulse sputtering, are compared. The changes in pattern size and morphology determined by the oxidation of the Ti patterns and Si substrate are analyzed. The hydrophilicity induced by UV-light irradiation and the visible-light photocatalytic activity towards the degradation of the methylene blue of the fabricated TiO2/SiO2 patterns were considerably higher when compared to the performances of uniform TiO2 films.

4.
Biomed Microdevices ; 15(3): 495-507, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23404262

RESUMO

Two methods for protein patterning on antifouling surfaces have been applied to analyze the density and bioactivity of the proteins after deposition. Microcontact printing has been used as a technique to transfer fibronectin through conformal contact, while piezoelectric deposition has been employed as a non-contact technique for producing arrays of fibronectin (FN). Plasma deposited polyethylene oxide-like (PEO-like) films have been used as non-fouling background to achieve the bioadhesive/biorepellent surface contrast. Both patterning methods allow the direct fabrication of protein arrays on a non-fouling substrate, and the subsequent formation of a pattern of stem cells by cell attachment on the arrayed substrates. Microcontact printing produced fully packed homogeneous fibronectin patterns, much denser than microspotted patterns. Both printing and spotting technologies generated functional protein arrays, their bioactivity being primarily modulated by the density of the deposited protein layer. Optimization of the FN parameters used for deposition has lead to the achievement of high-quality microarrays with large population of neural stem cells immobilized in the patterns in serum-free conditions, where cells exhibit a more homogeneous starting population and factors influencing fate decisions can be more easily tracked. The immunorecognition of fibronectin targeted antibodies, as well as the cell density, increase with the protein density up to a saturation point. Over 100 ng/cm² of fibronectin on the surface leads to a decrease in the number of attached cells and a raise of cell spreading.


Assuntos
Fibronectinas/química , Microtecnologia/métodos , Gases em Plasma/química , Polietilenoglicóis/química , Impressão/métodos , Células-Tronco/citologia , Adesivos/química , Fibronectinas/metabolismo , Humanos , Polimerização , Propriedades de Superfície
5.
Langmuir ; 29(52): 16098-104, 2013 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-24320829

RESUMO

This work reports results of an atomic force microscopy (AFM) study of adhesion force between hydroxylated AFM tips and supported lipid bilayers (SLBs) of phosphatidylcholine in phosphate buffer saline solution at neutral pH. Silicon nitride AFM probes were hydroxylated by treatment in water vapor plasma and used in force spectroscopy measurements of adhesion force on SLBs with control of contact loading force and residence time. The measurements showed a stochastic behavior of adhesion force that was attributed to stochastic formation of hydrogen bonds between the hydroxyl groups on the AFM tip and oxygen atoms from the phosphate groups of the phosphatidylcholine molecules. Analysis of a large number of force curves revealed a very low probability of hydrogen bond formation, a probability that increased with the increase of contact loading force and residence time. The variance and mean values of adhesion force showed a linear dependence on each other, which indicated that hydrogen bond formation obeyed the Poisson distribution of probability. This allowed for the quantitative determination of the rupture force per hydrogen bond of about 40 pN and showed the absence of other nonspecific interaction forces.


Assuntos
Bicamadas Lipídicas/química , Silicatos de Alumínio/química , Hidroxilação , Microscopia de Força Atômica , Modelos Biológicos , Propriedades de Superfície
6.
Langmuir ; 28(5): 2558-66, 2012 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-22229845

RESUMO

Transport mechanisms involved in capillary condensation of water menisci in nanoscopic gaps between hydrophilic surfaces are investigated theoretically and experimentally by atomic force microscopy (AFM) measurements of capillary force. The measurements showed an instantaneous formation of a water meniscus by coalescence of the water layers adsorbed on the AFM tip and sample surfaces, followed by a time evolution of meniscus toward a stationary state corresponding to thermodynamic equilibrium. This dynamics of the water meniscus is indicated by time evolution of the meniscus force, which increases with the contact time toward its equilibrium value. Two water transport mechanisms competing in this meniscus dynamics are considered: (1) Knudsen diffusion and condensation of water molecules in the nanoscopic gap and (2) adsorption of water molecules on the surface region around the contact and flow of the surface water toward the meniscus. For the case of very hydrophilic surfaces, the dominant role of surface water transportation on the meniscus dynamics is supported by the results of the AFM measurements of capillary force of water menisci formed at sliding tip-sample contacts. These measurements revealed that fast movement of the contact impedes on the formation of menisci at thermodynamic equilibrium because the flow of the surface water is too slow to reach the moving meniscus.

7.
Ultramicroscopy ; 109(3): 222-9, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19121899

RESUMO

The present paper shows that atomic force microscopy (AFM) imaging of friction force and phase lag in ambient air can be used to characterize the chemical contrast induced by electron beam (EB) irradiation on polyethylene glycol oxide (PEO) surface. Time-of-flight secondary emission mass spectroscopy measurements showed that the EB irradiation generates chemical contrast on PEO surface by decreasing the ether bond density. The AFM measurements showed smaller phase lag and lower friction and adhesive forces on the EB irradiated PEO surface, as compared to the non-irradiated PEO surface. While the chemical contrast in friction force had a linear dependence on the EB irradiation dose, the dependence of the chemical contrast in the phase lag was strongly non-linear. As the friction and adhesive forces depended on the AFM probe hydrophilicity and air humidity, the contrast in friction and adhesive forces is ascribed to different capillary condensation of ambient water vapour at the AFM tip contact with the EB irradiated and non-irradiated PEO surfaces, respectively.

9.
Nanotechnology ; 19(12): 125306, 2008 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-21817727

RESUMO

In this study we present a method to produce nanostructured surfaces containing bio-adhesive features inside a non bio-adhesive matrix. The strategy is based on the combination of low pressure plasma polymerization and electron beam lithography processes and allows the fabrication of the structured materials in just two steps without using any solvents. In a first step, a thin protein-and-cell-repelling coating (∼10 nm) is obtained by plasma polymerization of Di-glyme. Then, in a second step, the bio-adhesive properties of the layer are tuned by monitoring the concentration of ether bonds of the film by irradiating it locally by different irradiation doses with an electron beam. Time-of-flight secondary ion mass spectroscopy and atomic force microscopy analysis have been used to characterize the produced surfaces. Experiments with a model protein (bovine serum albumin) on the patterned surfaces show preferential adhesion to the irradiated regions, indicating the potential of this simple technique for the development of highly compacted sensitive bio-sensing devices.

10.
Biomaterials ; 29(36): 4766-74, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18819707

RESUMO

We present a method for patterning neural stem cells based on pre-patterning polypeptides on a cell-repellent surface (poly(ethylene) oxide-like, PEO-like, plasma-deposited films). The method ensures cell attachment and stability for several weeks, as well as it allows cell migration and differentiation. Various patterns of approximately 1 nm thick cell adhesive poly-L-lysine (PLL) have been created on a cell-repellent PEO-like matrix by microcontact printing using different array configurations and printing conditions. The cell-repellent property of PEO-like film determined the confinement of the cells on the printed patterns. Optimization of the printing method showed that the most homogeneous patterns over large areas were obtained using PLL diluted in carbonate buffer (100mM) at pH 8.4. Neural stem cells cultured on the PLL patterns in low serum and in differentiating medium over 20 days exhibited a good confinement to the polypeptide domains. The number of cells attached increased linearly with the micro-stamped PLL area. The cells were able to extend random axon-like projections to the outside of the patterns and presented high amount of ramifications when cultured in differentiating medium. Migration and axon-like outgrowth have been successfully guided by means of an interconnected squares configuration. The surfaces are suitable for controlling the patterning of stem cells and provide a platform for the assessment of the way how different cell arrangements and culture conditions influence cell interactions and cell developmental processes.


Assuntos
Neurônios/citologia , Células-Tronco/citologia , Adesividade , Contagem de Células , Movimento Celular , Proliferação de Células , Células Cultivadas , Fibronectinas/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Microscopia de Fluorescência , Polietilenoglicóis/metabolismo , Polilisina/metabolismo , Ressonância de Plasmônio de Superfície , Propriedades de Superfície , Cordão Umbilical/citologia
11.
Langmuir ; 22(3): 1093-8, 2006 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-16430270

RESUMO

Water bridges formed through capillary condensation at nanoscale contacts first stretch and then break during contact rupture. Atomic force microscopy (AFM) pull-off experiments performed in air with hydrophilic tips and samples show that stretched nanoscopic water bridges are in mechanical equilibrium with the external pull-off force acting at the contact but not in thermodynamic equilibrium with the water vapor in air. The experimental findings are explained by a theoretical model that considers constant water volume and decrease of water meniscus curvature during meniscus stretching. The model predicts that the water bridge breakup distance will be roughly equal to the cubic root of the water bridge volume. A thermodynamic instability was noticed for large water bridges formed at the contact of a blunt AFM tip (curvature radius of 400 nm) with a flat sample. In this case, experiments showed rise and stabilization of the volume of the water at the contact in about 1 s. For sharp AFM tips (curvature radius below 50 nm), the experiments indicated that formation of stable water bridges occurs in a much shorter time (below 5 ms).

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA