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1.
Trends Biochem Sci ; 46(12): 1017-1029, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34538727

RESUMO

Class A G protein-coupled receptors have evolved to recognize ligands ranging from small-molecule odorants to proteins. Although they are among the most diverse membrane receptors in eukaryotic organisms, they possess a highly conserved core within their seven-transmembrane helix framework. The conservation of the transmembrane core has led to the idea of a common mechanism by which ligand binding is coupled to the outward rotation of helix H6, the hallmark of an active receptor. Nevertheless, there is still no consensus on the mechanism of coupling or on the roles of specific residues within the core. Recent insights from crystallography and NMR spectroscopy provide a way to decompose the core into its essential structural and functional elements that shed new light on this important region.


Assuntos
Receptores Acoplados a Proteínas G , Ligantes , Espectroscopia de Ressonância Magnética , Receptores Acoplados a Proteínas G/metabolismo
2.
J Struct Biol ; 216(2): 108092, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38615725

RESUMO

Cerebral amyloid angiopathy (CAA) is associated with the accumulation of fibrillar Aß peptides upon and within the cerebral vasculature, which leads to loss of vascular integrity and contributes to disease progression in Alzheimer's disease (AD). We investigate the structure of human-derived Aß40 fibrils obtained from patients diagnosed with sporadic or familial Dutch-type (E22Q) CAA. Using cryo-EM, two primary structures are identified containing elements that have not been observed in in vitro Aß40 fibril structures. One population has an ordered N-terminal fold comprised of two ß-strands stabilized by electrostatic interactions involving D1, E22, D23 and K28. This charged cluster is disrupted in the second population, which exhibits a disordered N-terminus and is favored in fibrils derived from the familial Dutch-type CAA patient. These results illustrate differences between human-derived CAA and AD fibrils, and how familial CAA mutations can guide fibril formation.


Assuntos
Peptídeos beta-Amiloides , Angiopatia Amiloide Cerebral , Eletricidade Estática , Humanos , Peptídeos beta-Amiloides/metabolismo , Peptídeos beta-Amiloides/genética , Peptídeos beta-Amiloides/química , Angiopatia Amiloide Cerebral/patologia , Angiopatia Amiloide Cerebral/genética , Angiopatia Amiloide Cerebral/metabolismo , Microscopia Crioeletrônica/métodos , Amiloide/metabolismo , Amiloide/química , Amiloide/genética , Fragmentos de Peptídeos/genética , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/metabolismo , Mutação , Doença de Alzheimer/genética , Doença de Alzheimer/patologia , Doença de Alzheimer/metabolismo
3.
Biochemistry ; 61(12): 1181-1198, 2022 06 21.
Artigo em Inglês | MEDLINE | ID: mdl-35666749

RESUMO

Two distinct diseases are associated with the deposition of fibrillar amyloid-ß (Aß) peptides in the human brain in an age-dependent fashion. Alzheimer's disease is primarily associated with parenchymal plaque deposition of Aß42, while cerebral amyloid angiopathy (CAA) is associated with amyloid formation of predominantly Aß40 in the cerebral vasculature. In addition, familial mutations at positions 22 and 23 of the Aß sequence can enhance vascular deposition in the two major subtypes of CAA. The E22Q (Dutch) mutation is associated with CAA type 2, while the D23N (Iowa) mutation is associated with CAA type 1. Here we investigate differences in the formation and structure of fibrils of these mutant Aß peptides in vitro to gain insights into their biochemical and physiological differences in the brain. Using Fourier transform infrared and nuclear magnetic resonance spectroscopy, we measure the relative propensities of Aß40-Dutch and Aß40-Iowa to form antiparallel structure and compare these propensities to those of the wild-type Aß40 and Aß42 isoforms. We find that both Aß40-Dutch and Aß40-Iowa have strong propensities to form antiparallel ß-hairpins in the first step of the fibrillization process. However, there is a marked difference in the ability of these peptides to form elongated antiparallel structures. Importantly, we find marked differences in the stability of the protofibril or fibril states formed by the four Aß peptides. We discuss these differences with respect to the mechanisms of Aß fibril formation in CAA.


Assuntos
Doença de Alzheimer , Angiopatia Amiloide Cerebral , Amiloide , Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/genética , Angiopatia Amiloide Cerebral/genética , Angiopatia Amiloide Cerebral/patologia , Humanos , Iowa , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/genética , Placa Amiloide/patologia
4.
J Biol Chem ; 297(5): 101259, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34599967

RESUMO

The accumulation of fibrillar amyloid-ß (Aß) peptides alongside or within the cerebral vasculature is the hallmark of cerebral amyloid angiopathy (CAA). This condition commonly co-occurs with Alzheimer's disease (AD) and leads to cerebral microbleeds, intracranial hemorrhages, and stroke. CAA also occurs sporadically in an age-dependent fashion and can be accelerated by the presence of familial Aß mutant peptides. Recent studies using Fourier transform infrared (FTIR) spectroscopy of vascular Aß fibrils derived from rodents containing the double E22Q/D23N mutations indicated the presence of a novel antiparallel ß-sheet structure. To address whether this structure is associated solely with the familial mutations or is a common feature of CAA, we propagated Aß fibrils from human brain vascular tissue of patients diagnosed with nonfamilial CAA. Aß fibrils were isolated from cerebral blood vessels using laser capture microdissection in which specific amyloid deposits were removed from thin slices of the brain tissue. Transmission electron microscopy revealed that these deposits were organized into a tight meshwork of fibrils, which FTIR measurements showed could serve as seeds to propagate the growth of Aß40 fibrils for structural studies. Solid-state NMR measurements of the fibrils propagated from vascular amyloid showed they contained a mixture of parallel, in-register, and antiparallel ß-sheet structures. The presence of fibrils with antiparallel structure derived from vascular amyloid is distinct from the typical parallel, in-register ß-sheet structure that appears in fibrils derived from parenchymal amyloid in AD. These observations reveal that different microenvironments influence the structures of Aß fibrils in the human brain.


Assuntos
Doença de Alzheimer , Peptídeos beta-Amiloides , Encéfalo/metabolismo , Mutação de Sentido Incorreto , Fragmentos de Peptídeos , Idoso , Doença de Alzheimer/genética , Doença de Alzheimer/metabolismo , Substituição de Aminoácidos , Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/genética , Peptídeos beta-Amiloides/metabolismo , Humanos , Masculino , Ressonância Magnética Nuclear Biomolecular , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/genética , Fragmentos de Peptídeos/metabolismo
5.
Blood ; 135(12): 948-953, 2020 03 19.
Artigo em Inglês | MEDLINE | ID: mdl-31978223

RESUMO

Mutations in the MPL gene encoding the human thrombopoietin receptor (TpoR) drive sporadic and familial essential thrombocythemias (ETs). We identified 2 ET patients harboring double mutations in cis in MPL, namely, L498W-H499C and H499Y-S505N. Using biochemical and signaling assays along with partial saturation mutagenesis, we showed that L498W is an activating mutation potentiated by H499C and that H499C and H499Y enhance the activity of the canonical S505N mutation. L498W and H499C can activate a truncated TpoR mutant, which lacks the extracellular domain, indicating these mutations act on the transmembrane (TM) cytosolic domain. Using a protein complementation assay, we showed that L498W and H499C strongly drive dimerization of TpoR. Activation by tryptophan substitution is exquisitely specific for position 498. Using structure-guided mutagenesis, we identified upstream amino acid W491 as a key residue required for activation by L498W or canonical activating mutations such as S505N and W515K, as well as by eltrombopag. Structural data point to a common dimerization and activation path for TpoR via its TM domain that is shared between the small-molecule agonist eltrombopag and canonical and novel activating TpoR mutations that all depend on W491, a potentially accessible extracellular residue that could become a target for therapeutic intervention.


Assuntos
Benzoatos/farmacologia , Predisposição Genética para Doença , Hidrazinas/farmacologia , Mutação , Pirazóis/farmacologia , Receptores de Trombopoetina/agonistas , Receptores de Trombopoetina/genética , Trombocitemia Essencial/genética , Alelos , Substituição de Aminoácidos , Linhagem Celular , Estudos de Associação Genética , Humanos , Fenótipo , Transdução de Sinais/efeitos dos fármacos , Trombocitemia Essencial/diagnóstico , Trombocitemia Essencial/metabolismo
6.
J Biol Chem ; 295(27): 8914-8927, 2020 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-32376688

RESUMO

Cerebral amyloid angiopathy (CAA) is a vascular disorder that primarily involves deposition of the 40-residue-long ß-amyloid peptide (Aß40) in and along small blood vessels of the brain. CAA is often associated with Alzheimer's disease (AD), which is characterized by amyloid plaques in the brain parenchyma enriched in the Aß42 peptide. Several recent studies have suggested a structural origin that underlies the differences between the vascular amyloid deposits in CAA and the parenchymal plaques in AD. We previously have found that amyloid fibrils in vascular amyloid contain antiparallel ß-sheet, whereas previous studies by other researchers have reported parallel ß-sheet in fibrils from parenchymal amyloid. Using X-ray fluorescence microscopy, here we found that copper strongly co-localizes with vascular amyloid in human sporadic CAA and familial Iowa-type CAA brains compared with control brain blood vessels lacking amyloid deposits. We show that binding of Cu(II) ions to antiparallel fibrils can block the conversion of these fibrils to the more stable parallel, in-register conformation and enhances their ability to serve as templates for seeded growth. These results provide an explanation for how thermodynamically less stable antiparallel fibrils may form amyloid in or on cerebral vessels by using Cu(II) as a structural cofactor.


Assuntos
Peptídeos beta-Amiloides/metabolismo , Angiopatia Amiloide Cerebral/metabolismo , Cobre/metabolismo , Fragmentos de Peptídeos/metabolismo , Doença de Alzheimer/metabolismo , Amiloide/metabolismo , Peptídeos beta-Amiloides/fisiologia , Encéfalo/metabolismo , Angiopatia Amiloide Cerebral/fisiopatologia , Humanos , Espectroscopia de Ressonância Magnética/métodos , Microscopia de Força Atômica/métodos , Conformação Molecular , Fragmentos de Peptídeos/fisiologia , Placa Amiloide/metabolismo , Conformação Proteica em Folha beta
7.
Int J Mol Sci ; 22(3)2021 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-33513738

RESUMO

The amyloid-ß (Aß) peptides are associated with two prominent diseases in the brain, Alzheimer's disease (AD) and cerebral amyloid angiopathy (CAA). Aß42 is the dominant component of cored parenchymal plaques associated with AD, while Aß40 is the predominant component of vascular amyloid associated with CAA. There are familial CAA mutations at positions Glu22 and Asp23 that lead to aggressive Aß aggregation, drive vascular amyloid deposition and result in degradation of vascular membranes. In this study, we compared the transition of the monomeric Aß40-WT peptide into soluble oligomers and fibrils with the corresponding transitions of the Aß40-Dutch (E22Q), Aß40-Iowa (D23N) and Aß40-Dutch, Iowa (E22Q, D23N) mutants. FTIR measurements show that in a fashion similar to Aß40-WT, the familial CAA mutants form transient intermediates with anti-parallel ß-structure. This structure appears before the formation of cross-ß-sheet fibrils as determined by thioflavin T fluorescence and circular dichroism spectroscopy and occurs when AFM images reveal the presence of soluble oligomers and protofibrils. Although the anti-parallel ß-hairpin is a common intermediate on the pathway to Aß fibrils for the four peptides studied, the rate of conversion to cross-ß-sheet fibril structure differs for each.


Assuntos
Doença de Alzheimer/metabolismo , Peptídeos beta-Amiloides/química , Amiloide/química , Angiopatia Amiloide Cerebral/genética , Amiloide/metabolismo , Peptídeos beta-Amiloides/genética , Peptídeos beta-Amiloides/metabolismo , Benzotiazóis , Angiopatia Amiloide Cerebral/metabolismo , Dicroísmo Circular , Fluorescência , Microscopia de Força Atômica , Mutação , Placa Amiloide/genética , Placa Amiloide/metabolismo , Conformação Proteica em Folha beta/genética , Espectroscopia de Infravermelho com Transformada de Fourier
8.
J Biol Chem ; 294(15): 5854-5866, 2019 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-30755484

RESUMO

Extracellular deposition of ß-amyloid (Aß) peptides in the brain is a hallmark of Alzheimer's disease (AD). Upon ß-secretase-mediated cleavage of the ß C-terminal fragment (ß-CTF) from the Aß precursor protein, the γ-secretase complex produces the Aß peptides associated with AD. The familial T43I mutation within the transmembrane domain of the ß-CTF (also referred to as C99) increases the ratio between the Aß42 and Aß40 peptides largely due to a decrease in Aß40 formation. Aß42 is the principal component of amyloid deposits within the brain parenchyma, and an increase in the Aß42/Aß40 ratio is correlated with early-onset AD. Using NMR and FTIR spectroscopy, here we addressed how the T43I substitution influences the structure of C55, the minimal sequence containing the entire extracellular and transmembrane (TM) domains of C99 needed for γ-secretase processing. 13C NMR chemical shifts indicated that the T43I substitution increases helical structure within the TM domain of C55. These structural changes were associated with a shift of the C55 dimer to the monomer and an increase in the tilt of the TM helix relative to the membrane normal in the T43I mutant compared with that of WT C55. The A21G (Flemish) mutation was previously found to increase secreted Aß40 levels; here, we combined this mutation in the extracellular domain of C99 with T43I and observed that the T43I/A21G double mutant decreases Aß40 formation. We discuss how the observed structural changes in the T43I mutant may decrease Aß40 formation and increase the Aß42/Aß40 ratio.


Assuntos
Doença de Alzheimer , Secretases da Proteína Precursora do Amiloide/química , Peptídeos beta-Amiloides/química , Mutação de Sentido Incorreto , Fragmentos de Peptídeos/química , Peptídeos/química , Substituição de Aminoácidos , Secretases da Proteína Precursora do Amiloide/metabolismo , Peptídeos beta-Amiloides/genética , Peptídeos beta-Amiloides/metabolismo , Humanos , Ressonância Magnética Nuclear Biomolecular , Fragmentos de Peptídeos/genética , Fragmentos de Peptídeos/metabolismo , Peptídeos/genética , Peptídeos/metabolismo , Domínios Proteicos
9.
Am J Pathol ; 188(12): 2877-2889, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30446159

RESUMO

Accumulation of fibrillar amyloid ß protein in blood vessels of the brain, a condition known as cerebral amyloid angiopathy (CAA), is a common pathology of elderly individuals, a prominent comorbidity of Alzheimer disease, and a driver of vascular cognitive impairment and dementia. Although several transgenic mouse strains have been generated that develop varying levels of CAA, consistent models of associated cerebral microhemorrhage and vasculopathy observed clinically have been lacking. Reliable preclinical animal models of CAA and microhemorrhage are needed to investigate the molecular pathogenesis of this condition. Herein, we describe the generation and characterization of a novel transgenic rat (rTg-DI) that produces low levels of human familial CAA Dutch/Iowa E22Q/D23N mutant amyloid ß protein in brain and faithfully recapitulates many of the pathologic aspects of human small-vessel CAA. rTg-DI rats exhibit early-onset and progressive accumulation of cerebral microvascular fibrillar amyloid accompanied by early-onset and sustained behavioral deficits. Comparable to CAA in humans, the cerebral microvascular amyloid in rTg-DI rats causes capillary structural alterations, promotes prominent perivascular neuroinflammation, and produces consistent, robust microhemorrhages and small-vessel occlusions that are readily detected by magnetic resonance imaging. The rTg-DI rats provide a new model to investigate the pathogenesis of small-vessel CAA and microhemorrhages, to develop effective biomarkers for this condition and to test therapeutic interventions.


Assuntos
Peptídeos beta-Amiloides/metabolismo , Precursor de Proteína beta-Amiloide/genética , Encéfalo/patologia , Angiopatia Amiloide Cerebral/patologia , Mutação , Placa Amiloide/complicações , Peptídeos beta-Amiloides/genética , Animais , Comportamento Animal , Encéfalo/irrigação sanguínea , Encéfalo/metabolismo , Angiopatia Amiloide Cerebral/etiologia , Angiopatia Amiloide Cerebral/metabolismo , Humanos , Ratos , Ratos Transgênicos
10.
Biophys J ; 112(11): 2315-2326, 2017 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-28591604

RESUMO

G protein-coupled receptors (GPCRs) have evolved a seven-transmembrane helix framework that is responsive to a wide range of extracellular signals. An analysis of the interior packing of family A GPCR crystal structures reveals two clusters of highly packed residues that facilitate tight transmembrane helix association. These clusters are centered on amino acid positions 2.47 and 4.53, which are highly conserved as alanine and serine, respectively. Ala2.47 mediates the interaction between helices H1 and H2, while Ser4.53 mediates the interaction between helices H3 and H4. The helical interfaces outside of these clusters are lined with residues that are more loosely packed, a structural feature that facilitates motion of helices H5, H6, and H7, which is required for receptor activation. Mutation of the conserved small side chain at position 4.53 within packing cluster 2 is shown to disrupt the structure of the visual receptor rhodopsin, whereas sites in packing cluster 1 (e.g., positions 1.46 and 2.47) are more tolerant to mutation but affect the overall stability of the protein. These findings reveal a common structural scaffold of GPCRs that is important for receptor folding and activation.


Assuntos
Receptores Acoplados a Proteínas G/metabolismo , Sequência de Aminoácidos , Animais , Células COS , Ligação de Hidrogênio , Modelos Moleculares , Movimento (Física) , Mutação , Conformação Proteica , Dobramento de Proteína , Receptores Acoplados a Proteínas G/química , Receptores Acoplados a Proteínas G/genética , Rodopsina/química , Rodopsina/genética , Rodopsina/metabolismo
11.
J Biol Chem ; 291(6): 2974-87, 2016 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-26627830

RESUMO

Ligand binding to the extracellular domain of the thrombopoietin receptor (TpoR) imparts a specific orientation on the transmembrane (TM) and intracellular domains of the receptors that is required for physiologic activation via receptor dimerization. To map the inactive and active dimeric orientations of the TM helices, we performed asparagine (Asn)-scanning mutagenesis of the TM domains of the murine and human TpoR. Substitution of Asn at only one position (S505N) activated the human receptor, whereas Asn substitutions at several positions activated the murine receptor. Second site mutational studies indicate that His(499) near the N terminus of the TM domain is responsible for protecting the human receptor from activation by Asn mutations. Structural studies reveal that the sequence preceding His(499) is helical in the murine receptor but non-helical in peptides corresponding to the TM domain of the inactive human receptor. The activating S505N mutation and the small molecule agonist eltrombopag both induce helix in this region of the TM domain and are associated with dimerization and activation of the human receptor. Thus, His(499) regulates the activation of human TpoR and provides additional protection against activating mutations, such as oncogenic Asn mutations in the TM domain.


Assuntos
Proteínas Proto-Oncogênicas/metabolismo , Receptores de Trombopoetina/metabolismo , Animais , Asparagina/genética , Asparagina/metabolismo , Linhagem Celular , Histidina/genética , Histidina/metabolismo , Humanos , Camundongos , Mutação de Sentido Incorreto , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Proteínas Proto-Oncogênicas/genética , Receptores de Trombopoetina/genética
12.
J Biol Chem ; 290(11): 7169-84, 2015 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-25614624

RESUMO

Understanding the molecular mechanisms controlling the physiological and pathological activity of γ-secretase represents a challenging task in Alzheimer disease research. The assembly and proteolytic activity of this enzyme require the correct interaction of the 19 transmembrane domains (TMDs) present in its four subunits, including presenilin (PS1 or PS2), the γ-secretase catalytic core. GXXXG and GXXXG-like motifs are critical for TMDs interactions as well as for protein folding and assembly. The GXXXG motifs on γ-secretase subunits (e.g. APH-1) or on γ-secretase substrates (e.g. APP) are known to be involved in γ-secretase assembly and in Aß peptide production, respectively. We identified on PS1 and PS2 TMD8 two highly conserved AXXXAXXXG motifs. The presence of a mutation causing an inherited form of Alzheimer disease (familial Alzheimer disease) in the PS1 motif suggested their involvement in the physiopathological configuration of the γ-secretase complex. In this study, we targeted the role of these motifs on TMD8 of PSs, focusing on their role in PS assembly and catalytic activity. Each motif was mutated, and the impact on complex assembly, activity, and substrate docking was monitored. Different amino acid substitutions on the same motif resulted in opposite effects on γ-secretase activity, without affecting the assembly or significantly impairing the maturation of the complex. Our data suggest that AXXXAXXXG motifs in PS TMD8 are key determinants for the conformation of the mature γ-secretase complex, participating in the switch between the physiological and pathological functional conformations of the γ-secretase.


Assuntos
Secretases da Proteína Precursora do Amiloide/metabolismo , Presenilina-1/metabolismo , Presenilina-2/metabolismo , Sequência de Aminoácidos , Secretases da Proteína Precursora do Amiloide/química , Peptídeos beta-Amiloides/metabolismo , Animais , Células CHO , Linhagem Celular , Sequência Conservada , Cricetulus , Células HEK293 , Humanos , Camundongos , Dados de Sequência Molecular , Mutação , Presenilina-1/química , Presenilina-2/química , Estrutura Terciária de Proteína
13.
PLoS Genet ; 9(8): e1003700, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23966878

RESUMO

The spore wall of Saccharomyces cerevisiae is a multilaminar extracellular structure that is formed de novo in the course of sporulation. The outer layers of the spore wall provide spores with resistance to a wide variety of environmental stresses. The major components of the outer spore wall are the polysaccharide chitosan and a polymer formed from the di-amino acid dityrosine. Though the synthesis and export pathways for dityrosine have been described, genes directly involved in dityrosine polymerization and incorporation into the spore wall have not been identified. A synthetic gene array approach to identify new genes involved in outer spore wall synthesis revealed an interconnected network influencing dityrosine assembly. This network is highly redundant both for genes of different activities that compensate for the loss of each other and for related genes of overlapping activity. Several of the genes in this network have paralogs in the yeast genome and deletion of entire paralog sets is sufficient to severely reduce dityrosine fluorescence. Solid-state NMR analysis of partially purified outer spore walls identifies a novel component in spore walls from wild type that is absent in some of the paralog set mutants. Localization of gene products identified in the screen reveals an unexpected role for lipid droplets in outer spore wall formation.


Assuntos
Parede Celular/genética , Redes Reguladoras de Genes , Metabolismo dos Lipídeos/genética , Esporos Fúngicos/genética , Parede Celular/metabolismo , Quitosana/metabolismo , Regulação Fúngica da Expressão Gênica , Espectroscopia de Ressonância Magnética , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/crescimento & desenvolvimento , Esporos Fúngicos/crescimento & desenvolvimento , Esporos Fúngicos/metabolismo , Tirosina/análogos & derivados , Tirosina/genética , Tirosina/metabolismo
14.
Proc Natl Acad Sci U S A ; 110(5): 1646-51, 2013 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-23319611

RESUMO

The transmembrane (TM) and juxtamembrane (JM) regions of the ErbB family receptor tyrosine kinases connect the extracellular ligand-binding domain to the intracellular kinase domain. Evidence for the role of these regions in the mechanism of receptor dimerization and activation is provided by TM-JM peptides corresponding to the Neu (or rat ErbB2) receptor. Solid-state NMR and fluorescence spectroscopy show that there are tight interactions of the JM sequence with negatively charged lipids, including phosphatidylinositol 4,5-bisphosphate, in TM-JM peptides corresponding to the wild-type receptor sequence. We observe a release of the JM sequence from the negatively charged membrane surface using peptides containing an activating V664E mutation within the TM domain or in peptides engineered to form TM helix dimers with Val664 in the interface. These results provide the basis of a mechanism for coupling ligand binding to kinase activation in the full-length receptor.


Assuntos
Peptídeos/química , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Receptor ErbB-2/química , Sequência de Aminoácidos , Substituição de Aminoácidos , Animais , Calmodulina/química , Calmodulina/metabolismo , Cinética , Bicamadas Lipídicas/química , Bicamadas Lipídicas/metabolismo , Espectroscopia de Ressonância Magnética , Proteínas de Membrana/química , Proteínas de Membrana/metabolismo , Dados de Sequência Molecular , Mutação , Peptídeos/genética , Peptídeos/metabolismo , Ligação Proteica , Multimerização Proteica , Ratos , Receptor ErbB-2/genética , Receptor ErbB-2/metabolismo , Espectrometria de Fluorescência
15.
Proc Natl Acad Sci U S A ; 110(7): 2540-5, 2013 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-23359689

RESUMO

Dimerization of single-pass membrane receptors is essential for activation. In the human thrombopoietin receptor (TpoR), a unique amphipathic RWQFP motif separates the transmembrane (TM) and intracellular domains. Using a combination of mutagenesis, spectroscopy, and biochemical assays, we show that W515 of this motif impairs dimerization of the upstream TpoR TM helix. TpoR is unusual in that a specific residue is required for this inhibitory function, which prevents receptor self-activation. Mutations as diverse as W515K and W515L cause oncogenic activation of TpoR and lead to human myeloproliferative neoplasms. Two lines of evidence support a general mechanism in which W515 at the intracellular juxtamembrane boundary inhibits dimerization of the TpoR TM helix by increasing the helix tilt angle relative to the membrane bilayer normal, which prevents the formation of stabilizing TM dimer contacts. First, measurements using polarized infrared spectroscopy show that the isolated TM domain of the active W515K mutant has a helix tilt angle closer to the bilayer normal than that of the wild-type receptor. Second, we identify second-site R514W and Q516W mutations that reverse dimerization and tilt angle changes induced by the W515K and W515L mutations. The second-site mutations prevent constitutive activation of TpoR W515K/L, while preserving ligand-induced signaling. The ability of tryptophan to influence the angle and dimerization of the TM helix in wild-type TpoR and in the second-site revertants is likely associated with its strong preference to be buried in the headgroup region of membrane bilayers.


Assuntos
Modelos Moleculares , Conformação Proteica , Receptores de Trombopoetina/química , Receptores de Trombopoetina/metabolismo , Triptofano/metabolismo , Motivos de Aminoácidos/genética , Animais , Sequência de Bases , Western Blotting , Linhagem Celular , Clonagem Molecular , Dimerização , Citometria de Fluxo , Teste de Complementação Genética , Humanos , Luciferases , Espectroscopia de Ressonância Magnética , Camundongos , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Receptores de Trombopoetina/genética , Análise de Sequência de DNA , Espectrofotometria Infravermelho , Ultracentrifugação
16.
Biochemistry ; 54(27): 4197-207, 2015 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-26069943

RESUMO

Soluble oligomers and protofibrils of the Aß42 peptide are neurotoxic intermediates in the conversion of monomeric Aß42 into the amyloid fibrils associated with Alzheimer's disease. Nuclear magnetic resonance and Fourier transform infrared spectroscopy, along with single-touch atomic force microscopy, are used to establish the structural transitions involved in fibril formation. We show that under conditions favorable for the nucleated conformation conversion, the Aß42 peptide aggregates into largely unstructured low-molecular weight (MW) oligomers that are able to stack to form high-MW oligomers and to laterally associate to form protofibrils. ß-Sheet secondary structure develops during the irreversible lateral association of the oligomers. The first step in this conversion is the formation of an antiparallel ß-hairpin stabilized by intramonomer hydrogen bonding. The antiparallel ß-hairpins then associate into a cross ß-sheet structure with parallel and in-register ß-strands having intermonomer hydrogen bonding.


Assuntos
Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/ultraestrutura , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/ultraestrutura , Doença de Alzheimer/metabolismo , Peptídeos beta-Amiloides/metabolismo , Dicroísmo Circular , Humanos , Microscopia de Força Atômica , Ressonância Magnética Nuclear Biomolecular , Fragmentos de Peptídeos/metabolismo , Agregados Proteicos , Agregação Patológica de Proteínas/metabolismo , Estrutura Secundária de Proteína , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura
17.
J Biol Chem ; 289(25): 17895-908, 2014 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-24828504

RESUMO

The fibrillar assembly and deposition of amyloid ß (Aß) protein, a key pathology of Alzheimer disease, can occur in the form of parenchymal amyloid plaques and cerebral amyloid angiopathy (CAA). Familial forms of CAA exist in the absence of appreciable parenchymal amyloid pathology. The molecular interplay between parenchymal amyloid plaques and CAA is unclear. Here we investigated how early-onset parenchymal amyloid plaques impact the development of microvascular amyloid in transgenic mice. Tg-5xFAD mice, which produce non-mutated human Aß and develop early-onset parenchymal amyloid plaques, were bred to Tg-SwDI mice, which produce familial CAA mutant human Aß and develop cerebral microvascular amyloid. The bigenic mice presented with an elevated accumulation of Aß and fibrillar amyloid in the brain compared with either single transgenic line. Tg-SwDI/Tg-5xFAD mice were devoid of microvascular amyloid, the prominent pathology of Tg-SwDI mice, but exhibited larger parenchymal amyloid plaques compared with Tg-5xFAD mice. The larger parenchymal amyloid deposits were associated with a higher loss of cortical neurons and elevated activated microglia in the bigenic Tg-SwDI/Tg-5xFAD mice. The periphery of parenchymal amyloid plaques was largely composed of CAA mutant Aß. Non-mutated Aß fibril seeds promoted CAA mutant Aß fibril formation in vitro. Further, intrahippocampal administration of biotin-labeled CAA mutant Aß peptide accumulated on and adjacent to pre-existing parenchymal amyloid plaques in Tg-5xFAD mice. These findings indicate that early-onset parenchymal amyloid plaques can serve as a scaffold to capture CAA mutant Aß peptides and prevent their accumulation in cerebral microvessels.


Assuntos
Peptídeos beta-Amiloides/metabolismo , Angiopatia Amiloide Cerebral/metabolismo , Angiopatia Amiloide Cerebral/fisiopatologia , Córtex Cerebral/irrigação sanguínea , Córtex Cerebral/metabolismo , Circulação Cerebrovascular , Placa Amiloide/metabolismo , Peptídeos beta-Amiloides/genética , Animais , Angiopatia Amiloide Cerebral/genética , Angiopatia Amiloide Cerebral/patologia , Córtex Cerebral/patologia , Humanos , Camundongos , Camundongos Transgênicos , Mutação , Placa Amiloide/genética , Placa Amiloide/patologia
18.
Biochim Biophys Acta ; 1837(5): 683-93, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24183693

RESUMO

Rhodopsin is a classical two-state G protein-coupled receptor (GPCR). In the dark, its 11-cis retinal chromophore serves as an inverse agonist to lock the receptor in an inactive state. Retinal-protein and protein-protein interactions have evolved to reduce the basal activity of the receptor in order to achieve low dark noise in the visual system. In contrast, absorption of light triggers rapid isomerization of the retinal, which drives the conversion of the receptor to a fully active conformation. Several specific protein-protein interactions have evolved that maintain the lifetime of the active state in order to increase the sensitivity of this receptor for dim-light vision in vertebrates. In this article, we review the molecular interactions that stabilize rhodopsin in the dark-state and describe the use of solid-state NMR spectroscopy for probing the structural changes that occur upon light-activation. Amino acid conservation provides a guide for those interactions that are common in the class A GPCRs as well as those that are unique to the visual system. This article is part of a Special Issue entitled: Retinal Proteins - You can teach an old dog new tricks.


Assuntos
Sequência Conservada , Modelos Moleculares , Retinaldeído/química , Rodopsina/química , Sequência de Aminoácidos , Animais , Cristalografia por Raios X , Humanos , Ligação de Hidrogênio , Isomerismo , Luz , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Retinaldeído/metabolismo , Rodopsina/metabolismo
19.
EMBO J ; 30(21): 4398-413, 2011 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-21892137

RESUMO

Ligand binding to the thrombopoietin receptor is thought to stabilize an active receptor dimer that regulates megakaryocyte differentiation and platelet formation, as well as haematopoietic stem cell renewal. By fusing a dimeric coiled coil in all seven possible orientations to the thrombopoietin receptor transmembrane (TM)-cytoplasmic domains, we show that specific biological effects and in vivo phenotypes are imparted by distinct dimeric orientations, which can be visualized by cysteine mutagenesis and crosslinking. Using functional assays and computational searches, we identify one orientation that represents the inactive dimeric state and another similar to a physiologically activated receptor. Several other dimeric orientations are identified that induce proliferation and in vivo myeloproliferative and myelodysplastic disorders, indicating the receptor can signal from several dimeric interfaces. The set of dimeric thrombopoietin receptors with different TM orientations may offer new insights into the activation of distinct signalling pathways by a single receptor and suggests that subtle differences in cytokine receptor dimerization provide a new layer of signalling regulation that is relevant for disease.


Assuntos
Multimerização Proteica/fisiologia , Receptores de Trombopoetina/química , Receptores de Trombopoetina/metabolismo , Sequência de Aminoácidos , Animais , Células Cultivadas , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Modelos Biológicos , Modelos Moleculares , Dados de Sequência Molecular , Conformação Proteica , Domínios e Motivos de Interação entre Proteínas/genética , Domínios e Motivos de Interação entre Proteínas/fisiologia , Mapas de Interação de Proteínas , Multimerização Proteica/genética , Receptores de Trombopoetina/genética , Receptores de Trombopoetina/fisiologia , Transdução de Sinais/fisiologia , Estereoisomerismo
20.
Biochemistry ; 53(30): 5000-7, 2014 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-25010350

RESUMO

Activation of the protein tyrosine kinase receptors requires the coupling of ligand binding to a change in both the proximity and orientation of the single transmembrane (TM) helices of receptor monomers to allow transphosphorylation of the receptor kinase domain. We make use of peptides corresponding to the TM and juxtamembrane (JM) regions of the fibroblast growth factor receptor 3 to assess how mutations in the TM region (G380R and A391E), which lead to receptor activation, influence the orientation of the TM domain and interactions of the intracellular JM sequence with the membrane surface. On the basis of fluorescence and Fourier transform infrared spectroscopy, we find that both activating mutations change the TM helix tilt angle relative to the membrane normal and release the JM region from the membrane. These results suggest a general mechanism regarding how the TM-JM region functionally bridges the extracellular and intracellular regions for these receptors.


Assuntos
Membrana Celular/química , Receptor Tipo 3 de Fator de Crescimento de Fibroblastos/química , Sequência de Aminoácidos , Membrana Celular/genética , Membrana Celular/metabolismo , Humanos , Dados de Sequência Molecular , Fosforilação/genética , Ligação Proteica/genética , Multimerização Proteica/fisiologia , Estrutura Secundária de Proteína/genética , Receptor Tipo 3 de Fator de Crescimento de Fibroblastos/genética , Receptor Tipo 3 de Fator de Crescimento de Fibroblastos/metabolismo
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