RESUMO
The German Society of Pneumology initiated 2021 the AWMF S1 guideline Long COVID/Post-COVID. In a broad interdisciplinary approach, this S1 guideline was designed based on the current state of knowledge.The clinical recommendations describe current Long COVID/Post-COVID symptoms, diagnostic approaches, and therapies.In addition to the general and consensus introduction, a subject-specific approach was taken to summarize the current state of knowledge.The guideline has an explicit practical claim and will be developed and adapted by the author team based on the current increase in knowledge.
Assuntos
COVID-19 , Síndrome de COVID-19 Pós-Aguda , HumanosRESUMO
The mechanical properties of cells are known to be a label-free, inherent marker of biological function in health and disease. Wide-spread utilization has so far been impeded by the lack of a convenient measurement technique with sufficient throughput. To address this unmet need, we have recently introduced real-time deformability cytometry (RT-DC) for continuous mechanical single-cell classification of heterogeneous cell populations at rates of several hundred cells per second. Cells are driven through the constriction zone of a microfluidic chip leading to cell deformations due to hydrodynamic stresses only. Our custom-built image processing software performs image acquisition, image analysis and data storage on the fly. The ensuing deformations can be quantified and an analytical model enables the derivation of cell material properties. Performing RT-DC we highlight its potential to identify rare objects in heterogeneous suspensions and to track drug-induced changes in cells. In summary, RT-DC enables marker-free, quantitative phenotyping of heterogeneous cell populations with a throughput comparable to standard flow cytometry.
Assuntos
Citofotometria/instrumentação , Citofotometria/métodos , Desenho de Equipamento , Citometria de Fluxo/métodos , Células HL-60 , Ensaios de Triagem em Larga Escala/instrumentação , Ensaios de Triagem em Larga Escala/métodos , Humanos , Hidrodinâmica , Processamento de Imagem Assistida por Computador , Dispositivos Lab-On-A-ChipRESUMO
With an increasing incidence of the malignant melanoma we also have to expect an increasing number of metastases of the parotid lymph nodes. Up to now there are no definite guidelines concerning the surgical treatment of these metastases. What is important is the question whether the facial nerve should be resected or retained. Between 1987 and 1991 in the ENT-clinic of the University Hamburg-Eppendorf eight patients were treated for metastases of a malignant melanoma in the parotid gland. The clinical course demonstrates that there is no indication for a radical surgical treatment of these patients. It neither leads to a delayed appearance of distant metastases, nor does it improve the life expectancy.