Biochar symbiosis in anaerobic digestion to enhance biogas production: A comprehensive review.

In recent years, anaerobic digestion (AD) has gained popularity as a practical method for generating clean energy and efficiently managing organic waste. However, the effectiveness of the reactor is compromised by the accumulation of ammonia, acids, and nutrients, leading to inhibition and instability. Because of its adaptability, biochar (BC) has sparked a substantial interest in biogas production and can be created by charring biomass and waste materials. Adding BC to the AD process could yield the following benefits: mitigating toxic inhibition, reducing the duration of the methanogenic lag phase, immobilising functional bacteria, and enhancing the rate of electron transfer between methanogenic and acetogenic microorganisms. Nonetheless, there remains to be more comprehensive knowledge regarding the multifaceted function of BC and its intricate mechanisms in the generation of biogas in AD. The research summarises scattered information from the literature on BC production from various feedstocks and factors affecting its characteristics. Additionally, a comprehensive analysis of the utilisation of BC as an additive within AD is presented here, emphasising how BC characteristics impact AD processes and how they effectively engage key challenges.

Rice mill wastewater management in the era of circular economy.

Several nations around the world use rice as their primary food staple because of its tremendous nutritional value. India's expanding population has sparked a proliferation of rice mills as a result of the country's growing rice demand. However, small and medium-scale industries lack adequate facilities for processing effluents and other waste generated. Paddy is typically processed by parboiling, which involves soaking it in water, boiling it with steam, and then drying and milling. Around 1-1.5 L of water is necessary to partially cook 1 kg of unhusked rice, with approximately half of this water being discharged as effluent. Disposal of rice mill effluent (RME) in water bodies or on the land causes severe damage to soil and water. An inclusive examination of diverse approaches for the treatment and stabilization of partially cooked rice milling effluents is provided. Moreover, the document provides a concise overview of contemporary and environmentally friendly technologies for treating RME. Adsorption, electrocoagulation, chemical coagulation, and bioremediation using microbes, plants, and microalgae are all included in these methods. This manuscript discusses the concept of a circular economy, which is focused on enhancing environmental sustainability through the recycling and repurposing of generated waste into raw materials for the creation of new products. In addition, this review aims to focus on the impact of RME on soils and water species and the status of sustainable management at the point of circular economy with RME bioenergy production (bioelectricity, biomethane, and bio-hydrogen).

Advancements in laundry wastewater treatment for reuse: a review.

Laundry related activities produce huge quantity of wastewater that is very rich in lint, dyes, surfactants, and detergents. The large amount of laundry wastewater (LWW) is generated by extensive human activities. LWW needs to be treated in order to tackle the challenging problem of water pollution and to accomplish water sustainability. To achieve this success, LWW can be reused for several purposes such as irrigation, construction activities, vehicle washing etc. However, there are several challenges in the reclamation of LWW like effective handling of the wastewater and meeting the regulatory criteria. Based on the literature review it seems that a single treatment process is not sufficient to treat LWW up to acceptable reuse standards. To that end, different treatment chains have been proposed: i) hybrid processes combining membrane filtration with adsorption; ii) advanced oxidation process using ultraviolet (UV) and hydrogen peroxide (H2O2); iii) coagulation-flocculation combined with sand filtration; iv) combination of ozonation process, adsorption, and ultrafiltration (UF). This review paper discusses the selection of suitable treatment technology depends on several factors: i) a well-designed equipment, ii) cost-effectiveness of treatment method and iii) desired characteristics of the treated water. The review paper also presents solutions for treatment and reclamation of LWW.

Environmental applications of microbial extracellular polymeric substance (EPS): A review.

During the last decade, water demand and wastewater generation has increased due to urbanization around the globe which had led to an increase in the utilization of chemicals/synthetic polymers for treating the wastewaters. These synthetic polymers used during the coagulation/flocculation process are non-renewable, non-biodegradable, and have a potential neurotoxic and carcinogenic effect. From the literature it is clear that extracellular polymer substance (EPS) is a potential bioflocculant, moreover it is renewable, biodegradable, eco-friendly, non-toxic as well as economically valued product. The various identification techniques and extraction methods of EPS are elaborated. Further application of EPS as absorbent in removing the dye from the industrial effluent is presented. Moreover EPS as a potential adsorbent for heavy metal removal from the various effluent is discussed. In addition, EPS is also utilized for soil remediation and soil erosion control. Mainly, EPS as bioflocculant in treating raw water, wastewater treatment, leachate and sludge management are summarized in this review.

Energy balance assessment on chicken manure for biogas production in Rabat-Salé-Zemmour-Zaïr of Morocco.

Chicken manure management has grabbed significant attention in Morocco due to the increasing demand on chicken and eggs. Bioconversion of chicken manure to biogas could reduce the chicken manure amount as well as generate clean energy. To evaluate the feasibility of converting chicken manure to biogas in terms of energy gain, the energy balance of the chicken manure for biogas production in the region of Rabat-Salé-Zemmour-Zaïr of Morocco has been investigated. The result showed that there was an energy gain of 1350 MJ for per tonne of dry chicken manure was converted to biogas. The energy gain increased to 3996 MJ/tonne of dry chicken manure when the wheat straw was added to co-digest with chicken manure. With consideration of converting the obtained biogas for electricity generation, there was extra electricity for sale after subtracting the electricity consumed inside the poultry industry. Comparing with co-firing, pyrolysis, and gasification, chicken manure anaerobic digestion was superior in terms of energy gain.

Ultra-sonication for controlling the formation of disinfection by-products in the ClO2 pre-oxidation of water containing high concentrations of algae.

Eutrophication has become great concern in recent years due to the fact that rivers, lakes, and reservoirs are the main drinking water source. Studies have been performed to enhance the removal of algae with ClO2 pre-oxidation, but there was high potential in the formation of chlorite and chlorate. In this study, ultra-sonication was employed to assist algae removal and control disinfection by-products formation in ClO2 pre-oxidation processes. It was found that solo ultra-sonication for 10 min (algae removal 86.11 ± 2.16%) could achieve similar algae removal efficiency as that with solo ClO2 (0.5 mg/L) pre-oxidation for 10 min (algae removal 87.10 ± 3.50%). In addition, no formations of chlorite and chlorate were detected in solo ultra-sonication process. Five-minutes ultra-sonication followed by 5-min 0.5 mg/L ClO2 treatment (total treatment time 10 min; algae removal 93.55 ± 3.22%) provided a better performance on algae removal compared to the solo ClO2 (0.5 mg/L) pre-oxidation for 10 min. Moreover, chlorite was undetectable. It suggests that the utilization of ultra-sonication in ClO2 pretreatment for algae removal has highly prevented the formations of chlorite and chlorate.

The potential utilization of slag generated from iron- and steelmaking industries: a review.

Along with iron and steel production, large amount of slag is generated. Proper management on the iron- and steelmaking slag is highly demanded due to the high cost of direct disposal of the slag to landfill, which is the most adopted management approach. In this article, the potential application of iron- and steelmaking slag has been reviewed, which included the slag utilization in construction as cement and sand, in water, soil, and gas treatment, as well as in value material recovery. In addition, the challenge and required effort to be made in iron- and steelmaking slag management have been discussed.

Techno-economic evaluation of simultaneous production of extra-cellular polymeric substance (EPS) and lipids by Cloacibacterium normanense NK6 using crude glycerol and sludge as substrate.

This study used the technical, economic analysis tool, SuperPro designer in evaluating a novel technology for simultaneous production of extracellular polymeric substance (EPS) and biodiesel using crude glycerol and secondary sludge. As renewable energy sources are depleting, the process utilizes municipal sewage sludge for production of EPS and biodiesel along with crude glycerol, which is a waste byproduct of biodiesel industry providing an alternate way for disposal of municipal sludge and crude glycerol. Newly isolated Cloacibacterium normanense NK6 is used as micro-organism in the study as it is capable of producing high EPS concentration, using activated sludge and crude glycerol as the sole carbon source. The technology has many environmental and economic advantages like the simultaneous production of two major products: EPS and lipids. Sensitivity analysis of the process revealed that biomass lipid content is a most significant factor where unit cost production of biodiesel was highly sensitive to lipid content during bioreaction. B7 biodiesel unit production cost can be lowered from $1 to $0.6 if the lipid content of the biomass is improved by various process parameter modifications.

Biopolymer Production Kinetics of Mixed Culture Using Wastewater Sludge as a Raw Material and the Effect of Different Cations on Biopolymer Applications in Water and Wastewater Treatment.

Thirteen extracellular polymeric substances (EPS) producing bacterial strains were cultivated (as mixed culture) in the sterilized sludge (suspended solids of 25 g/L) and the batch fermentation was carried out. Mixed culture revealed a high specific growth rate of 0.35/hr. The EPS production rate was higher up to 24 hours, which gradually decreased with further incubation. The kinetic estimates demonstrated growth-associated EPS production. Broth EPS revealed higher flocculation activity when combined with different cations (Ca(2+), Mg(2+), Fe(3+), and Al(3+)) in river water (≥90%), municipal wastewater (≥90%), and brewery wastewater (≥80%), respectively. A low dose (5 to 40 mg/L) of trivalent cations was required to achieve higher flocculation compared to the divalent cations (50 to 250 mg/L). Flocculation performance of EPS was comparable to Magnafloc-155 (chemical polymer) and, hence, it could be used as a flocculant.

Anaerobic digestion of ultrasonicated sludge at different solids concentrations - Computation of mass-energy balance and greenhouse gas emissions.

Two cases of anaerobic digestion (AD) of sludge, namely (i) with pre-treatment and (ii) without pre-treatment, were assessed using mass-energy balance and the corresponding greenhouse gas (GHG) emissions. For a digestion period of 30 days, volatile solids degradation of the control sludge and the ultrasonicated secondary sludge was 51.4% and 60.1%, respectively. Mass balance revealed that the quantity of digestate required for dewatering, transport and land application was the lowest (20.2 × 10(6) g dry sludge/day) for ultrasonicated secondary sludge at 31.4 g TS/L. Furthermore, for ultrasonicated secondary sludge at 31.4 g TS/L, the maximum net energy (energy output - energy input) of total dry solids (TDS) was 7.89 × 10(-6) kWh/g and the energy ratio (output/input) was 1.0. GHG emissions were also reduced with an increase in the sludge solids concentration (i.e., 40.0 g TS/L < 30.0 g TS/L < 20.0 g TS/L). Ultrasonication pre-treatment proved to be efficient and beneficial for enhancing anaerobic digestion efficiency of the secondary sludge when compared to the primary and mixed sludge.

Recovery of residual soluble protein by two-step precipitation process with concomitant COD reduction from the yeast-cultivated cheese whey.

The present study was conducted to recover the residual soluble protein after cultivation of yeast (K. marxianus) in cheese whey. Cheese whey continuous fermentation with cell recycle system was carried out at 40 °C and pH 3.5. The yeast biomass was separated from the fermented broth by centrifugation and residual soluble protein from fermented whey supernatant was precipitated by heat treatment (at 100 °C, pH 4.5 and 10 min incubation). The maximum soluble protein recovery up to 53 % was achieved at pH 4.5 with 54 % residual COD removal. However, gravity sedimentable precipitates were obtained at pH 3.5 with 47 % protein recovery. Therefore, the reactor (scale up) study was conducted at pH 3.5 with agitation, which resulted in 68 % of residual soluble protein recovery and simultaneously residual COD removal of 62 %. Further precipitation/coagulation of soluble protein was also evaluated using carboxymethylcellulose (CMC) and then two precipitation (thermal followed by CMC precipitation) processes were combined to increase the protein precipitation, which finally reached up to 81 % of total soluble protein recovery from the supernatant. This optimized process could be applied to recover the residual protein left after fermentation of cheese whey without centrifugation.

Simultaneous single-cell protein production and COD removal with characterization of residual protein and intermediate metabolites during whey fermentation by K. marxianus.

Cheese whey fermentation with Kluyveromyces marxianus was carried out at 40 °C and pH 3.5 to examine simultaneous single-cell protein production and chemical oxygen demand (COD) removal, determine the fate of soluble whey protein and characterize intermediate metabolites. After 36 h of batch fermentation, the biomass concentration increased from 2.0 to 6.0 g/L with 55 % COD reduction (including protein), whereas soluble whey protein concentration decreased from 5.6 to 4.1 g/L. It was confirmed through electrophoresis (SDS-PAGE) that the fermented whey protein was different from native whey protein. HPLC and GC-MS analysis revealed a change in composition of organic compounds post-fermentation. High inoculum concentration in batch fermentation resulted in an increase in biomass concentration from 10.3 to 15.9 g/L with 80 % COD reduction (including protein) within 36 h with residual protein concentration of 4.5 g/L. In third batch fermentation, the biomass concentration increased from 7.3 to 12.4 g/L with 71 % of COD removal and residual protein concentration of 4.3 g/L after 22 h. After 22 h, the batch process was shifted to a continuous process with cell recycle, and the steady state was achieved after another 60 h with biomass yield of 0.19 g biomass/g lactose and productivity of 0.26 g/L h. COD removal efficiency was 78-79 % with residual protein concentration of 3.8-4.2 g/L. The aerobic continuous fermentation process with cell recycle could be applied to single-cell protein production with substantial COD removal at low pH and high temperature from cheese whey.

Extracellular polymeric substances of bacteria and their potential environmental applications.

Biopolymers are considered a potential alternative to conventional chemical polymers because of their ease of biodegradability, high efficiency, non-toxicity and non-secondary pollution. Recently, extracellular polymeric substances (EPS, biopolymers produced by the microorganisms) have been recognised by many researchers as a potential flocculent for their applications in various water, wastewater and sludge treatment processes. In this context, literature information on EPS is widely dispersed and is very scarce. Thus, this review marginalizes various studies conducted so far about EPS nature-production-recovery, properties, environmental applications and moreover, critically examines future research needs and advanced application prospective of the EPS. One of the most important aspect of chemical composition and structural details of different moieties of EPS in terms of carbohydrates, proteins, extracellular DNA, lipid and surfactants and humic substances are described. These chemical characteristics of EPS in relation to formation and properties of microbial aggregates as well as degradation of EPS in the matrix (biomass, flocs etc) are analyzed. The important engineering properties (based on structural characteristics) such as adsorption, biodegradability, hydrophilicity/hydrophobicity of EPS matrix are also discussed in details. Different aspects of EPS production process such as bacterial strain maintenance; inoculum and factors affecting EPS production were presented. The important factors affecting EPS production include growth phase, carbon and nitrogen sources and their ratio, role of other nutrients (phosphorus, micronutrients/trace elements, and vitamins), impact of pH, temperature, metals, aerobic versus anaerobic conditions and pure and mixed culture. The production of EPS in high concentration with high productivity is essential due to economic reasons. Therefore, the knowledge about all the aspects of EPS production (listed above) is highly essential to formulate a logical and scientific basis for the research and industrial activities. One of the very important issues in the production/application/biodegradation of EPS is how the EPS is extracted from the matrix or a culture broth. Moreover, EPS matrix available in different forms (crude, loosely bound, tightly bound, slime, capsular and purified) can be used as a bioflocculant material. Several chemical and physical methods for the extraction of EPS (crude form or purified form) from different sources have been analyzed and reported. There is ample information available in the literature about various EPS extraction methods. Flocculability, dewaterability and biosorption ability are the very attractive engineering properties of the EPS matrix. Recent information on important aspects of these properties qualitatively as well as quantitatively has been described. Recent information on the mechanism of flocculation mediated by EPS is presented. Potential role of EPS in sludge dewatering and biosorption phenomenon has been discussed in details. Different factors influencing the EPS ability to flocculate and dewaterability of different suspensions have been included. The factors considered for the discussion are cations, different forms of EPS, concentration of EPS, protein and carbohydrate content of EPS, molecular weight of EPS, pH of the suspension, temperature etc. These factors were selected for the study based upon their role in the flocculation and dewatering mechanism as well the most recent available literature findings on these factors. For example, only recently it has been demonstrated that there is an optimum EPS concentration for sludge flocculation/dewatering. High or low concentration of EPS can lead to destabilization of flocs. Role of EPS in environmental applications such as water treatment, wastewater flocculation and settling, colour removal from wastewater, sludge dewatering, metal removal and recovery, removal of toxic organic compounds, landfill leachate treatment, soil remediation and reclamation has been presented based on the most recent available information. However, data available on environmental application of EPS are very limited. Investigations are required for exploring the potential of field applications of EPS. Finally, the limitations in the knowledge gap are outlined and the research needs as well as future perspectives are highlighted.

Predictive modelling of methane yield in biochar-amended cheese whey and septage co-digestion: Exploring synergistic effects using Gompertz and neural networks.

This study performed bench scale studies on anaerobic co-digestion of cheese whey and septage mixed with biochar (BC) as additive at various dosages (0.5 g, 1 g, 2 g and 4 g) and total solids (TS) concentrations (5%, 7.5%, 10%,12.5% and 15%). The experimental results revealed 29.58% increase in methane yield (486 ± 11.32 mL/gVS) with 27% reduction in lag phase time at 10% TS concentration and 50 g/L of BC loading. The mechanistic investigations revealed that BC improved process stability by virtue of its robust buffering capacity and mitigated ammonia inhibition. Statistical analysis indicates BC dosage had a more pronounced effect (P < 0.0001) compared to the impact of TS concentrations. Additionally, the results were modelled using Gompertz model (GM) and artificial neural network (ANN) algorithm, which revealed the outperformance of ANN over GM with MSE 17.96, R2 value 0.9942 and error 0.27%. These findings validated the practicality of utilizing a high dosage of BC in semi-solid anaerobic digestion conditions.

Odor control in lagoons.

Lagoons are widely used in rural area for wastewater treatment; however, the odor problem has hampered its application. The root of odor emission from lagoons varies from one to another. The key of controlling the odor is to find out the cause and accordingly provide strategies. Various physical, chemical, and biological methods have been reported and applied for odor control. Physical technologies such as masking, capturing and sorption are often employed to mitigate the pressure from compliant while not to cut off the problem. Chemical technologies which act rapidly and efficiently in odor control, utilize chemicals to damage the odorant production root or convert odorant to odorless substances. Biological methods such as aeration, biocover and biofiltration control the odor by enhancing aerobic condition or developing methanogens in lagoon, and biologically decomposing the odorants. Comparing to physical and chemical methods, biological methods are more feasible.

Di 2-ethylhexylphtalate in the aquatic and terrestrial environment: a critical review.

Phthalates are being increasingly used as softeners-plasticizers to improve the plasticity and the flexibility of materials. Amongst the different plasticizers used, more attention is paid to di (2-ethylhexylphtalate) (DEHP), one of the most representative compounds as it exhibits predominant effects on environment and human health. Meanwhile, several questions related to its sources; toxicity, distribution and fate still remain unanswered. Most of the evidence until date suggests that DEHP is an omnipresent compound found in different ecological compartments and its higher hydrophobicity and low volatility have resulted in significant adsorption to solids matrix. In fact, there are important issues to be addressed with regard to the toxicity of this compound in both animals and humans, its behavior in different ecological systems, and the transformation products generated during different biological or advanced chemical treatments. This article presents detailed review of existing treatment schemes, research gaps and future trends related to DEHP.

Production of extracellular polymeric substances (EPS) by Serratia sp.1 using wastewater sludge as raw material and flocculation activity of the EPS produced.

Growth profile and extracellular polymeric substances (EPS) production of Serratia sp.1 was studied in shake flask fermentation for 72 h using wastewater sludge as raw material. Maximum cell concentration of 6.7 × 10(9) cfu/mL was obtained at 48 h fermentation time. EPS dry weight, flocculation activity and dewaterability of different EPS (tightly bound or TB-EPS, loosely bound or LB-EPS and broth-EPS or B-EPS) were also measured. The highest concentration of LB-EPS (2.45 g/L) and TB-EPS (0.99 g/L) were attained at 48 h of fermentation. Maximum flocculation activity and dewaterability (ΔCST) of TB-EPS (76.4%, 14.5s and 76.5%, 15.5s), LB-EPS (67.8%, 8.1s and 64.7%, 7.6s) and broth EPS (61%, 6.1s and 70.4%, 6.8s) were obtained at 36 and 48 h of growth. Higher flocculation activity and dewaterability were achieved with TB-EPS than with the two other EPS. Characterization of TB-EPS and LB-EPS was done in terms of their protein and carbohydrate content. Protein content was much higher in TB-EPS where as carbohydrate content was only slightly higher in TB-EPS than LB-EPS. Morphology of the Serratia strain after fermentation in sludge and TSB was observed under a scanning electron microscope and the cell size was found to be bigger in the sludge medium than the TSB medium.

Saccharomyces unisporus: Biotechnological Potential and Present Status.

The yeast species of the Saccharomyces genus have a long history of traditional applications and beneficial effects. Among these presence of the Saccharomyces unisporus has been documented in various dairy products and has become a subject of interest and great importance. S. unisporus has shown a significant role in the ripening of cheese and production of fermented milk products such as kefir and koumiss. The absence of pseudohyphae during the life cycle of S. unisporus is an indication of nonpathogenicity. Significance has been laid on the presence of S. unisporus in food-grade products and a close proximity of S. unisporus to S. florentinus and both of these species are accepted by the International Dairy Federation and the European Food and Feed Cultures Association for food and feed applications. Since over the years, S. unisporus has already become a part of various dairy products, S. unisporus can be considered as a potential candidate for generally regarded as safe status. S. unisporus has the capacity to convert ketoisophorone to levodione, which is an important pharmaceutical precursor. S. unisporus are considered as the potential producers of farnesol which eventually controls filamentation of pathogenic microorganisms. Apart from that, S. unisporus produces certain omega unsaturated fatty acids which combat diseases. Henceforth, the areas which S. unisporus can be possibly exploited for its useful intermediates are the enzymes and fatty acids it produces. In this context, this review attempts to describe and discuss the ubiquity of S. unisporus in food products, cellular composition, regulatory pathways, and its synthesis of fatty acids and enzymes.

Nanoplastics removal from spiked laundry wastewater using electro-peroxidation process.

Microplastics and nanoplastics (NPs) in laundry wastewater (LWW) are major sources of plastic particles in wastewater treatment plants. Unlike microplastics, almost no information exists in the literature on the degradation of NPs in LWW. In this work, the degradation of NPs in commercial LWW by the electro-peroxidation process is investigated. The obtained results demonstrated that already existing ions in LWW such as Cl- contribute to faster degradation of NPs and a complete removal could be obtained as fast as 40 min. In addition, three-dimensional excitation and emission matrix fluorescence analysis was performed, which revealed humic acid-like, aromatic proteins-like, and fulvic acid-like compounds could be oxidized after 20, 40, and 60 min of treatment respectively. The effects of operating parameters on the process performance were then examined by response surface methodology (RSM) models. The results showed that initial TOC concentration was the most important parameter influencing negatively the percentage of NP degradation. Afterward, optimization of the process revealed that the energy consumption could be minimized at 31.2 mA/cm2, 0.025 mol/L [Na2SO4], and 52 min treatment time for 52.2 mg/L initial TOC. Finally, analysis of treated LWW showed no toxicity on Daphnia magna. This study showed that the electro-peroxidation process can completely degrade NPs in LWW without any remaining toxic compounds.

Trends and challenges in the valorization of kitchen waste to polyhydroxyalkanoates.

Kitchen waste (KW) is frequently available for free or with a negative cost due to its huge production. It contains a large proportion of organic substances, especially fermentable sugars, which can be used for bioplastic (polyhydroxyalkanoates or PHA) synthesis. Nevertheless, due to the difficulties in processing, various pre-treatments of KW are being investigated to enhance the concentration of simple sugars released during its hydrolysis. The effective use of KW will help in minimizing the issues of its inappropriate disposal. However, the review on KW to bioplastic synthesis is rarely reported in the literature. Hence, this particular review provides a comprehensive summary of the updated research developments in KW valorization and its potency as a feedstock for PHAs synthesis. Additionally, the impacts of KW, its availability, the necessary pre-treatments for the biopolymerization process, as well as the prospects and challenges for industrially generating sustainable PHAs, are critically discussed.