Effect of water temperature on the morbidity of Tetracapsuloides bryosalmonae (Myxozoa) to brown trout (Salmo trutta) under laboratory conditions.

Proliferative kidney disease (PKD) is a disease found in salmonid fish that is widespread in Europe and North America. The dependency of the clinical signs on the water temperature is extensively reported in rainbow trout, but detailed information on brown trout is lacking. In this study, juvenile brown trout were exposed to the spores of Tetracapsuloides bryosalmonae and then kept at different ambient water temperatures (16°C, 19°C and 22°C) for 10 weeks along with recording of morbidity throughout the experiment. At 6, 8 and 10 weeks post-exposure, fish from each temperature group were sampled and underwent pathoanatomical examination to survey disease progression. At 16°C, brown trout showed a significantly higher survival probability compared to those kept in 19°C and 22°C water. Additionally, the parasitic burden (MSQ) was higher and the clinical signs were more pronounced in the brown trout kept at 19°C and 22°C compared with the ones kept at 16°C. This study highlights the correlation of PKD outbreaks and water temperature increases related to global climate change, which will impact the future distribution of brown trout in natural waters.

Response of European grayling, Thymallus thymallus, to multiple stressors in hydropeaking rivers.

Rivers of the large Alpine valleys constitute iconic ecosystems that are highly threatened by multiple anthropogenic stressors. This stressor mix, however, makes it difficult to develop and refine conservation and restoration strategies. It is, therefore, urgent to acquire more detailed knowledge on the consequences and interactions of prevalent stressors on fish populations, in particular, on indicator species such as the European grayling Thymallus thymallus. Here, we conducted a multi-river, multi-stressor investigation to analyze the population status of grayling. Using explorative decision-tree approaches, we disentangled the main and interaction effects of four prevalent stressor groups: flow modification (i.e., hydropeaking), channelization, fragmentation, and water quality alteration. Moreover, using a modified variant of the bootstrapping method, pooled bootstrapping, we determined the optimal number of characteristics that adequately describe fish population status. In our dataset, hydropeaking had the strongest single effect on grayling populations. Grayling biomass at hydrological control sites was around eight times higher than at sites affected by hydropeaking. The primary parameters for predicting population status were downramping rate and peak amplitude, with critical ranges of 0.2-0.4 cm min-1 and 10-25 cm. In hydropeaking rivers, river morphology and connectivity were the preceding subordinated parameters. Repeating the procedure with pooled bootstrapping datasets strengthened the hypothesis that the identified parameters are most relevant in predicting grayling population status. Hence, hydropeaking mitigation based on ecological thresholds is key to protect and restore already threatened grayling populations. In hydropeaking rivers, high river network connectivity and heterogenous habitat features can dampen the adverse effects of pulsed-flow releases by offering shelter and habitats for all life cycle stages of fish. The presented approach of explorative tree analysis followed by post-hoc tests of identified effects, as well as the pooled bootstrapping method, offers a simple framework for researchers and managers to analyze multi-factorial datasets and draw solid management conclusions.

Predicting presence and absence of trout (Salmo trutta) in Iran.

Species distribution modelling, as a central issue in freshwater ecology, is an important tool for conservation and management of aquatic ecosystems. The brown trout (Salmo trutta) is a sensitive species which reacts to habitat changes induced by human impacts. Therefore, the identification of suitable habitats is essential. This study explores the potential distribution of brown trout by a species distribution modelling approach for Iran. Furthermore, modelling results are compared to the distribution described in the literature. Areas outside the currently known distribution which may offer potential habitats for brown trout are identified. The species distribution modelling was based on five different modelling techniques: Generalised Linear Model, Generalised Additive Model, Generalised Boosting Model, Classification Tree Analysis and Random Forests, which are finally summarised in an ensemble forecasting approach. We considered four environmental descriptors at the local scale (slope, bankfull width, wetted width, and elevation) and three climatic parameters (mean air temperature, range of air temperature and annual precipitation) which were extracted on three different spatial extents (1/5/10 km). The performance of all models was excellent (≥0.8) according to the TSS (True Skill Statistic) criterion. Slope, mean and range of air temperature were the most important variables in predicting brown trout occurrence. Presented results deepen the knowledge about distribution patterns of brown trout in Iran. Moreover, this study gives a basic background for the future development of assessment methods for riverine ecosystems in Iran.

Morphodynamic effects on the habitat of juvenile cyprinids (Chondrostoma nasus) in a restored Austrian lowland river.

At the Sulm River, an Austrian lowland river, an ecologically orientated flood protection project was carried out from 1998-2000. Habitat modeling over a subsequent 3-year monitoring program (2001-2003) helped assess the effects of river bed embankment and of initiating a new meander by constructing a side channel and allowing self-developing side erosion. Hydrodynamic and physical habitat models were combined with fish-ecological methods. The results show a strong influence of riverbed dynamics on the habitat quality and quantity for the juvenile age classes (0+, 1+, 2+) of nase (Chondrostoma nasus), a key fish species of the Sulm River. The morphological conditions modified by floods changed significantly and decreased the amount of weighted usable areas. The primary factor was river bed aggradation, especially along the inner bend of the meander. This was a consequence of the reduced sediment transport capacity due to channel widening in the modeling area. The higher flow velocities and shallower depths, combined with the steeper bank angle, reduced the Weighted Useable Areas (WUAs) of habitats for juvenile nase. The modeling results were evaluated by combining results of mesohabitat-fishing surveys and habitat quality assessments. Both, the modeling and the fishing results demonstrated a reduced suitability of the habitats after the morphological modifications, but the situation was still improved compared to the pre-restoration conditions at the Sulm River.