Antithrombotic effect of ligustrazine hydrochloride injection on the model of induced arteriovenous shunt thrombosis.

BACKGROUND: The objective of this study is to optimize the effective dose of heparin and ligustrazine hydrochloride injection (LHI) for drug combination. MATERIALS AND METHODS: The animal clinical study of LHI was performed by the rat's model of induced arteriovenous shunt thrombosis. Experimental animals were grouped into several groups and separately treated with both LHI (20, 40, 80 mg/kg, i.p.) and heparin (60, 55, and 50 U/kg; 5000 U/ml; Sigma, i.v). The study had used thrombus weight, protein concentration in thrombus homogenate, inhibition rate of thrombosis, and plasma anti-thrombin activity as indications. RESULTS: The group combination (50, 80) got the result of 100% antithrombotic activity with 0 ± 0 mg of thrombus weight, 14 ± 3 µg/ml of protein concentration in thrombus homogenate and 1.5 ± 0.04 U/ml of plasma anti-thrombin activity. Its anti-thrombotic effect was much better than individual groups treated with LHI in a dose of 0 mg/kg and group of combination (0, 80) (P < 0.05) while antithrombotic effect of 55 and 60 U/kg heparin alone was only 37-58%. Therefore, the group of combination (50, 80) was optimal for 100% antithrombotic activity. CONCLUSION: Optimal combined doses of LHI and heparin preventing blood coagulation were determined and the results were available. It may give some hint for the further clinical application on human.

[Effect of Auricularia auricula polysaccharide on PBMCs proliferation and cytokine expression in vitro].

OBJECTIVE: To investigate the proliferation and cytokine expression of Auricularia auricula polysaccharide (AAP) on peripheral blood monnuclear cells (PBMCs) in vitro. METHODS: AAP was obtained by water extraction and alcohol precipitation, and purified by DEAE-cellulose chromatogram. PBMCs were treated with AAP at different levels. The Cell Counting Kit-8 (CCK-8) was used to determine the proliferation of PBMCs. The concentration of TNF-alpha and TFN-gamma in supernatant were investigated by ELISA. RESULTS: The three fractions of AAP could stimulate PBMCs proliferation at the range of 100--800 microg/ml, and AAP2 enhanced significantly the level of TNF-alpha and IFN-gamma in supernatant of PBMCs (P < 0.05, P < 0.01). CONCLUSION: AAP is an immunomodulator and can enhance the immunomodulatory activity through TNF-alpha and TFN-gamma.