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Proteomics ; 5(13): 3262-77, 2005 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16052621

RESUMO

There is a substantial list of pre-analytical variables that can alter the analysis of blood-derived samples. We have undertaken studies on some of these issues including choice of sample type, stability during storage, use of protease inhibitors, and clinical standardization. As there is a wide range of sample variables and a broad spectrum of analytical techniques in the HUPO PPP effort, it is not possible to define a single list of pre-analytical standards for samples or their processing. We present here a compendium of observations, drawing on actual results and sound clinical theories and practices. Based on our data, we find that (1) platelet-depleted plasma is preferable to serum for certain peptidomic studies; (2) samples should be aliquoted and stored preferably in liquid nitrogen; (3) the addition of protease inhibitors is recommended, but should be incorporated early and used judiciously, as some form non specific protein adducts and others interfere with peptide studies. Further, (4) the diligent tracking of pre-analytical variables and (5) the use of reference materials for quality control and quality assurance, are recommended. These findings help provide guidance on sample handling issues, with the overall suggestion being to be conscious of all possible pre-analytical variables as a prerequisite of any proteomic study.


Assuntos
Proteínas Sanguíneas/química , Coleta de Amostras Sanguíneas/métodos , Proteômica/métodos , Proteômica/normas , Manejo de Espécimes/métodos , Manejo de Espécimes/normas , Plaquetas/química , Preservação de Sangue , Biologia Computacional , Eletroforese em Gel Bidimensional , Eletroforese em Gel de Poliacrilamida , Feminino , Humanos , Masculino , Análise de Sequência com Séries de Oligonucleotídeos , Peptídeos/química , Inibidores de Proteases/farmacologia , Análise Serial de Proteínas , Controle de Qualidade , Padrões de Referência , Valores de Referência , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Temperatura , Fatores de Tempo , Tripsina/farmacologia
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