Genome Analysis of Pseudoloma neurophilia: A Microsporidian Parasite of Zebrafish (Danio rerio).

Microsporidia are highly successful parasites that infect virtually all known animal lineages, including the model Danio rerio (zebrafish). The widespread use of this aquatic model for biomedical research has resulted in an unexpected increase in infections from the microsporidium Pseudoloma neurophilia, which can lead to significant physical, behavioral, and immunological modifications, resulting in nonprotocol variation during experimental procedures. Here, we seek to obtain insights into the biology of P. neurophilia by investigating its genome content, which was obtained from only 29 nanograms of DNA using the MiSeq technology and paired-end Illumina sequencing. We found that the genome of P. neurophilia is phylogenetically and genetically related to other fish-microsporidians, but features unique to this intracellular parasite are also found. The small 5.25-Mb genome assembly includes 1,139 unique open-reading frames and an unusually high number of transposable elements for such a small genome. Investigations of intragenomic diversity also provided strong indications that the mononucleate nucleus of this species is diploid. Overall, our study provides insights into the dynamics of microsporidian genomes and a solid sequence reference to be used in future studies of host-parasite interactions using the zebrafish D. rerio and P. neurophilia as a model.

Is it dangerous? The role of an emotional visual search strategy and threat-relevant training in the detection of guns and knives.

Counter-terrorism strategies rely on the assumption that it is possible to increase threat detection by providing explicit verbal instructions to orient people's attention to dangerous objects and hostile behaviours in their environment. Nevertheless, whether verbal cues can be used to enhance threat detection performance under laboratory conditions is currently unclear. In Experiment 1, student participants were required to detect a picture of a dangerous or neutral object embedded within a visual search display on the basis of an emotional strategy 'is it dangerous?' or a semantic strategy 'is it an object?'. The results showed a threat superiority effect that was enhanced by the emotional visual search strategy. In Experiment 2, whilst trainee police officers displayed a greater threat superiority effect than student controls, both groups benefitted from performing the task under the emotional than semantic visual search strategy. Manipulating situational threat levels (high vs. low) in the experimental instructions had no effect on visual search performance. The current findings provide new support for the language-as-context hypothesis. They are also consistent with a dual-processing account of threat detection involving a verbally mediated route in working memory and the deployment of a visual template developed as a function of training.

Can threat detection be enhanced using processing strategies by police trainees and officers?

The ability to detect threatening stimuli is an important skill for police officers. No research has yet examined whether implementing different information processing strategies can improve threat detection in police officers and police trainees. The first aim of our study was to compare the effect of strategies accentuating the processing of the emotional or the semantic dimension of stimuli on attention towards threatening and neutral information. The second aim was to consider the impact of PTSD symptoms on threat detection, as a function of processing strategies, in police officers and trainees. In a cueing paradigm, participants had to respond to a target that was presented following a threatening or neutral cue. Participants then answered a question, known beforehand, concerning the cue. The question was used to induce a more emotional or semantic processing strategy. Results showed that when the processing strategy was emotional, police trainees and officers were faster to detect the target when it followed a threatening cue, compared to a neutral cue, independently of its spatial location. This was not the case when the processing strategy was semantic. This study shows that induced processing strategies can influence attentional mechanisms related to threat detection in police trainees and police officers.

State anxiety modulates the impact of peripherally presented affective stimuli on foveal processing.

BACKGROUND: The priority processing of peripherally presented affective stimuli was recently shown in healthy individuals to divert attentional resources dedicated to foveal processing. Here we investigated the influence of sub-clinical levels of anxiety and depression on this bias. METHODS: Eighty-four participants were submitted to psychological tests that evaluate anxiety and depression levels. Then, they had to make speeded responses to the direction of left- or right-oriented arrows that were presented foveally at fixation. Each arrow was preceded by a peripherally presented pair of pictures, one neutral and one emotional, unpleasant or pleasant. Thus, the direction of the foveal arrow was either congruent or not with the peripheral location of the previously presented emotional picture. Data analysis focused on the differences of reaction times between congruent and incongruent conditions, which assess the spatial response bias in the task. RESULTS: A main effect of state-anxiety was observed suggesting that the higher the level of state-anxiety, the greater the congruence effect. LIMITATIONS: Since the obtained result relates to subclinical anxiety levels, its generalization to anxiety disorders remains tentative. CONCLUSIONS: State-anxiety appears to modulate the propensity to be influenced by emotionally salient information occurring in peripheral vision, independently of its relevance to the ongoing behavior. The long-term persistence of a high level of alertness for emotional cues in visual periphery could contribute to the causation and the maintenance of anxiety disorders.