Extraction, Structural Analysis, and Biofunctional Properties of Exopolysaccharide from Lactiplantibacillus pentosus B8 Isolated from Sichuan Pickle

Two novel exopolysaccharides, named LPB8-0 and LPB8-1, were isolated and purified from Lactiplantibacillus pentosus B8. Moreover, their structure and bioactivities were evaluated through chemical and spectral means. The study results demonstrated that LPB8-0 was primarily composed of mannose and glucose and had an average molecular weight of 1.12 × 104 Da, while LPB8-1 was composed of mannose, glucose, and galactose and had an average molecular weight of 1.78 × 105 Da. Their carbohydrate contents were 96.2% ± 1.0% and 99.1% ± 0.5%, respectively. The backbone of LPB8-1 was composed of (1→2)-linked α-D-Manp and (1→6)-linked α-D-Manp. LPB8-0 and LPB8-1 had semicrystalline structures with good thermal stability (308.3 and 311.7 °C, respectively). SEM results displayed that both LPB8-0 and LPB8-1 had irregular thin-slice shapes and spherical body structures. Additionally, an emulsifying ability assay confirmed that LPB8-0 and LPB8-1 had good emulsifying activity against several edible oils, and this activity was retained under acidic, neutral, and high temperature conditions. Furthermore, an antioxidant assay confirmed that LPB8-1 had stronger scavenging activity than LPB8-0. Overall, these results provide a theoretical basis for the potential application of these two novel exopolysaccharides as natural antioxidants and emulsifiers in the food and pharmaceutical industries.

Biochemical and genetic basis of cadmium biosorption by Enterobacter ludwigii LY6, isolated from industrial contaminated soil.

In this study, a cadmium-tolerant bacterium, Enterobacter ludwigii LY6, was isolated from cadmium-contaminated soil in Shifang, Sichuan province, China. The cadmium chloride removal rate of the strain LY6 with a treatment of 100 mg/L cadmium chloride reached 56.0%. Scanning electron microscopy showed that exopolysaccharides (EPS) might be the main means of cadmium adsorption by the strain. X-ray powder diffraction (XRD) and energy-dispersive X-ray spectroscopy (EDS) analyses indicated that cadmium sulfide nanoparticles formed on the surface of bacteria cultured in a medium containing 100 mg/L cadmium chloride. In addition, the expression of several genes increased with the increase of the cadmium concentration in the medium, including the multiple antibiotic resistance proteins marA and marR, and the cold shock protein CspA. GO functions, such as the redox activity, respiratory chain and transport functions, and KEGG pathways involved in "bacterial chemotaxis" and "terpenoid backbone biosynthesis" were found to be closely related to bacterial cadmium tolerance and biosorption. This is the first report that E. ludwigii can reduce sulfate to form cadmium sulfide nanoparticles under high concentration cadmium exposure. The genes related to cadmium tolerance identified in this study lay a foundation for the genetic breeding of cadmium-tolerant strains.