RESUMO
In this study, luciferase reporter assay was used to establish the relationship between miR-210 and ISCU. This research was performed on both cell lines (A2058, G361, and 293T) and tissue samples. We found that miR-210 was upregulated in hypoxia and was elevated in melanoma in comparison with adjacent normal tissues. Expression of ISCU protein was decreased in melanoma tissues, and ISCU gene is a direct target of miR-210. ISCU knockdown with miR-210 enhanced ROS production. The results of our study showed that miR-210/ISCU/ROS axis can serve as a novel therapeutic target in melanoma.
Assuntos
Proteínas Ferro-Enxofre , Melanoma , MicroRNAs , Apoptose/genética , Linhagem Celular Tumoral , Proliferação de Células/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Hipóxia/genética , Proteínas Ferro-Enxofre/genética , Melanoma/genética , Melanoma/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Compostos de SulfonilureiaRESUMO
The aim of this study was to investigate whether there were sex differences in the electromyograms (EMG) of the masticatory muscles and in the mandibular movement of young adults when chewing food. Twenty subjects with normal dentitions were selected. The 11 test foods selected were all easily available in Japan. The results showed firstly that although there were no significant differences of chewing frequency between males and females, females chew food more slowly with less masticatory force and narrower mouth opening width than males; and secondly it appears that the chewing function in females is lower than in males.