Genome-wide kinase-MAM interactome screening reveals the role of CK2A1 in MAM Ca2+ dynamics linked to DEE66.

The endoplasmic reticulum (ER) and mitochondria form a unique subcellular compartment called mitochondria-associated ER membranes (MAMs). Disruption of MAMs impairs Ca2+ homeostasis, triggering pleiotropic effects in the neuronal system. Genome-wide kinase-MAM interactome screening identifies casein kinase 2 alpha 1 (CK2A1) as a regulator of composition and Ca2+ transport of MAMs. CK2A1-mediated phosphorylation of PACS2 at Ser207/208/213 facilitates MAM localization of the CK2A1-PACS2-PKD2 complex, regulating PKD2-dependent mitochondrial Ca2+ influx. We further reveal that mutations of PACS2 (E209K and E211K) associated with developmental and epileptic encephalopathy-66 (DEE66) impair MAM integrity through the disturbance of PACS2 phosphorylation at Ser207/208/213. This, in turn, causes the reduction of mitochondrial Ca2+ uptake and the dramatic increase of the cytosolic Ca2+ level, thereby, inducing neurotransmitter release at the axon boutons of glutamatergic neurons. In conclusion, our findings suggest a molecular mechanism that MAM alterations induced by pathological PACS2 mutations modulate Ca2+-dependent neurotransmitter release.

A study on smartphone dependence and depression in Korean high school students.

Using the 10th Korean Youth Risk Behavior Web-based Survey in 2020, we attempted to determine the relationship between smartphone addiction and depression in 25,987 high school students. The demographic characteristics of the subjects were reviewed frequently, and the correlation between smartphone dependence and depression were determined. The results show that it is difficult to track the time spent on smartphones to determine the level of depression (R = 0.143, P < .01), it is difficult concentrating on other tasks due to smartphones (R = 0.140, P < .01), and it is difficult to remove images/data received on smartphones from the head (R = 0.141, P < .01). Further, the impulse to use smartphones is strong (R = 0.157, P < .01), health concerns (r = .124, P < .01) and family conflicts exist due to the use of smartphones (r = .149, P < .01), problems with social relations exist due to the use of smartphones (R = 112, P < .01), and difficulties exist in performing tasks due to the use of smartphones (R = 0.153, P < .01). According to the results of the study, it was determined that there is a relationship between smartphone usage and depression among Korean high school students. The findings from this study can be referenced to help guide the development of smartphone usage parameters for Korean teenagers in depression management programs.

[Determining factors of intention to actual use of charged long-term care services for the aged].

OBJECTIVES: To help develop strategies to cope with the changes arising from the rapid aging process by predicting the determining factors of intention to actual use of the charged long-term care services for elderly as perceived by the middle aged who play the major role of supports. METHODS: Subjects were the parents (men 177, women 507) in their 40s of the students selected from a university of Busan city. A questionnaire survey was conducted for 4 weeks in October 2003 about the knowledge for long-term care service, the intention of actual use, and the preferences about the type of service suppliers. Data analysis was performed with frequency, chi-square test, and t-test using SPSS program (ver 10.0K), along with data mining using decision tree of Enterprise Miner V8.2 by SAS. RESULTS: About half of the subjects (53.7%) had the actual experiences of elderly supports. Intentions to use the charged services were relatively high in home visiting nursing care service (40.1%) and long-term care facilities service (40.4%), and were influenced by previous knowledge about the services. The intentions were stronger in women, those with higher education, and those with greater income levels. Actual elderly supports were mc (80%) done by women, and the perceived burdens for supports were bigger in women and those of lower s economic level. Desired charges were about 10,000 for the bath service, 20,000 won for the rests services day, and about 500,000 won for the long-term care facil service per month. From the result of decision analysis, the job professionalism was the most impol determining factor of intention to actual use of the serv with validation as 63-71%. Health and welfare mixed facilities were preferred, and the most impor consideration was the level of professionalism. CONCLUSIONS: Intention to actual use of the chad services was largely determined by the aspects of time cost. Polices to increase the number of service supp and to decrease the burdens perceived by ac supporters were strongly recommended.