RESUMO
This study proposes an air circulation system that can forcibly circulate the lowest cold air to the top of indoor smart farms, and it has a width, length, and height of 6, 12, and 2.5 m, respectively, to reduce the effect of temperature differences between the upper and lower parts on the growth rate of plants in winter. This study also aimed to reduce the temperature deviation generated between the upper and lower parts of the target indoor space by optimizing the shape of the manufactured outlet of the air circulation system. A table of L9 orthogonal arrays, which is a design of experiment methodology, was used, and it presented three levels of the following design variables: blade angle, blade number, output height, and flow radius. Flow analysis was performed for the experiments on the nine models to minimize the high time and cost requirements. Based on the derived analysis results, an optimized prototype was manufactured by applying the Taguchi method, and experiments were conducted by installing 54 temperature points in an indoor space to identify the temperature difference between the upper and lower parts over time for the performance experiment. Under natural convection, the minimum temperature deviation was 2.2 °C and the temperature difference between the upper and lower parts did not decrease. For a model without an outlet shape, such as a vertical fan, the minimum temperature deviation was 0.8 °C and at least 530 s were required to reach a difference of less than 2 °C. When air was circulated in the air circulation system with the proposed outlet shape, the minimum temperature deviation was 0.6 °C and the time required to reach a difference of less than 2 °C was 440 s. Using the proposed air circulation system, cooling and heating costs are expected to be reduced in summer and winter because the arrival time and temperature difference between the upper and lower parts can be reduced using the outlet shape compared with the case without the outlet shape.
RESUMO
Accumulation of expanded polyglutamine proteins is considered to be a major pathogenic biomarker of Huntington disease. We isolated SCAMP5 as a novel regulator of cellular accumulation of expanded polyglutamine track protein using cell-based aggregation assays. Ectopic expression of SCAMP5 augments the formation of ubiquitin-positive and detergent-resistant aggregates of mutant huntingtin (mtHTT). Expression of SCAMP5 is markedly increased in the striatum of Huntington disease patients and is induced in cultured striatal neurons by endoplasmic reticulum (ER) stress or by mtHTT. The increase of SCAMP5 impairs endocytosis, which in turn enhances mtHTT aggregation. On the contrary, down-regulation of SCAMP5 alleviates ER stress-induced mtHTT aggregation and endocytosis inhibition. Moreover, stereotactic injection into the striatum and intraperitoneal injection of tunicamycin significantly increase mtHTT aggregation in the striatum of R6/2 mice and in the cortex of N171-82Q mice, respectively. Taken together, these results suggest that exposure to ER stress increases SCAMP5 in the striatum, which positively regulates mtHTT aggregation via the endocytosis pathway.