RESUMO
Herein, a highly stable aggregation-induced emission (AIE) fluorescent nanodot assembled by an amphiphilic quinoxalinone derivative-peptide conjugate, namely Quino-1-Fmoc-RACR (also termed as Q1-PEP), which exhibits large Stokes shift and an endoplasmic reticulum (ER)-targeting capacity for bioimaging is reported. It is found that the resulting nanodot can effectively enter the ER with high fluorescent emission. As the ER is mainly involved in the transport of synthesized proteins in vesicles to the Golgi or lysosomes, the Q1-PEP nanodot with ER-targeting capacity can be used to monitor vesicular transport inside the cells. Compared to conventional fluorescent dyes with small Stokes shifts, the self-assembled fluorescent nanodot shows superior resistance to photobleaching and aggregation-induced fluorescence quenching, and elimination of the spectra overlap with autofluorescence of biosubstrate owning to their AIE-active and red fluorescence emission characteristics. All these optical properties make the fluorescent nanodot suitable for noninvasive and long-term imaging both in vitro and in vivo.
Assuntos
Retículo Endoplasmático/metabolismo , Corantes Fluorescentes/química , Nanopartículas/química , Imagem Óptica/métodos , Vesículas Transportadoras/metabolismo , Animais , Autofagia , Humanos , Células MCF-7 , Camundongos Nus , Nanopartículas/ultraestrutura , Peptídeos/químicaRESUMO
Aggregation-induced emission (AIE) has been harnessed in many systems through the principle of restriction of intramolecular rotations (RIR) based on mechanistic understanding from archetypal AIE molecules such as tetraphenylethene (TPE). However, as the family of AIE-active molecules grows, the RIR model cannot fully explain some AIE phenomena. Here, we report a broadening of the AIE mechanism through analysis of 10,10',11,11'-tetrahydro-5,5'-bidibenzo[a,d][7]annulenylidene (THBDBA), and 5,5'-bidibenzo[a,d][7]annulenylidene (BDBA). Analyses of the computational QM/MM model reveal that the novel mechanism behind the AIE of THBDBA and BDBA is the restriction of intramolecular vibration (RIV). A more generalized mechanistic understanding of AIE results by combining RIR and RIV into the principle of restriction of intramolecular motions (RIM).
RESUMO
A silicon-containing hyperbranched polymer (hb-P1/2) with σ*-π* conjugation was prepared in a good yield and high molecular weight by rhodium-catalyzed alkyne polyhydrosilylation of 1,2-bis(4-ethynylphenyl)-1,2-diphenylethene (1) with tris(4-dimethylsilylphenyl)amine (2). The polymer was thermally stable, losing merely 5% of its weight when heated to ≈445 °C. Whereas hb-P1/2 was weakly luminescent when molecularly dissolved, it became highly emissive when supramolecularly aggregated, showing an aggregation-induced emission (AIE) phenomenon. A superamplification effect was observed when the AIE nanoaggregates were used as fluorescent chemosensor for explosive detection: the quenching efficiency was greatly increased in a nonlinear fashion with increasing quencher concentration.
RESUMO
Molecules of a thiol-functionalized phenylacetylene derivative were assembled on the CdS nanorod surface and copolymerized with phenylacetylene, affording an inorganic semiconductor-conjugated polymer hybrid with excellent solubility and high photoconductivity.
Assuntos
Química Click/métodos , Luminescência , Nanopartículas/química , Dióxido de Silício/química , Coloides , Etanol/química , Etilenos/química , Células HeLa , Humanos , Nanopartículas/ultraestrutura , Tamanho da Partícula , Soluções , Espectrometria de Fluorescência , Espectrofotometria Infravermelho , Eletricidade EstáticaRESUMO
A highly photoresponsive perovskite hybrid containing an electroactive organic component (H1) was fabricated. A disubstituted polyacetylene (PA) with a hidden amino functionality (P3) was synthesized, hydrolysis and quaternization of which afforded the desired PA ammonium salt (P5). Mixing P5 with lead bromide readily yielded H1, which was stable, soluble, and film-forming. The inorganic framework induced the polymer chains to align in an ordered fashion, which helped to populate the chain segments with long conjugation lengths. The hybrid emitted a blue light (457 nm) in a high quantum yield (62%), thanks to the enhanced electronic conjugation, the weakened interaction between the layer-segregated chains, and the efficient energy transfer from the inorganic sheets to the organic layers. P3 exhibited a half-discharge time as short as approximately 0.7 s, representing the first example of an efficient photoconductive disubstituted PA. While stable to normal light illumination, H1 was rapidly bleached upon exposure to high-power UV irradiation, enabling facile generation of two-dimensional luminescent photopatterns. After the UV irradiation, the emissions of P9 and P9/H12 were enhanced and weakened, respectively, proving that the inorganic perovskite framework works as a photocatalyst for accelerating the bleaching process of the conjugated PA chains.
RESUMO
Triphenylamine-functionalized tetraphenylethene shows aggregation-induced emission feature with unity solid-state fluorescence efficiency. Its amorphous film can function in a p-type FET device with field effect mobility up to 2.6 × 10(-3) cm(2)/Vs.