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1.
Proc Natl Acad Sci U S A ; 120(34): e2302910120, 2023 08 22.
Artigo em Inglês | MEDLINE | ID: mdl-37579143

RESUMO

Gene editing in the brain has been challenging because of the restricted transport imposed by the blood-brain barrier (BBB). Current approaches mainly rely on local injection to bypass the BBB. However, such administration is highly invasive and not amenable to treating certain delicate regions of the brain. We demonstrate a safe and effective gene editing technique by using focused ultrasound (FUS) to transiently open the BBB for the transport of intravenously delivered CRISPR/Cas9 machinery to the brain.


Assuntos
Encéfalo , Edição de Genes , Encéfalo/diagnóstico por imagem , Barreira Hematoencefálica , Transporte Biológico , Microbolhas
2.
Anal Chem ; 96(19): 7634-7642, 2024 05 14.
Artigo em Inglês | MEDLINE | ID: mdl-38691624

RESUMO

Chemical derivatization is a widely employed strategy in metabolomics to enhance metabolite coverage by improving chromatographic behavior and increasing the ionization rates in mass spectroscopy (MS). However, derivatization might complicate MS data, posing challenges for data mining due to the lack of a corresponding benchmark database. To address this issue, we developed a triple-dimensional combinatorial derivatization strategy for nontargeted metabolomics. This strategy utilizes three structurally similar derivatization reagents and is supported by MS-TDF software for accelerated data processing. Notably, simultaneous derivatization of specific metabolite functional groups in biological samples produced compounds with stable but distinct chromatographic retention times and mass numbers, facilitating discrimination by MS-TDF, an in-house MS data processing software. In this study, carbonyl analogues in human plasma were derivatized using a combination of three hydrazide-based derivatization reagents: 2-hydrazinopyridine, 2-hydrazino-5-methylpyridine, and 2-hydrazino-5-cyanopyridine (6-hydrazinonicotinonitrile). This approach was applied to identify potential carbonyl biomarkers in lung cancer. Analysis and validation of human plasma samples demonstrated that our strategy improved the recognition accuracy of metabolites and reduced the risk of false positives, providing a useful method for nontargeted metabolomics studies. The MATLAB code for MS-TDF is available on GitHub at https://github.com/CaixiaYuan/MS-TDF.


Assuntos
Metabolômica , Software , Humanos , Metabolômica/métodos , Neoplasias Pulmonares/metabolismo , Piridinas/química
3.
FASEB J ; 37(7): e22967, 2023 07.
Artigo em Inglês | MEDLINE | ID: mdl-37269155

RESUMO

Neutrophils participate in the pathogenesis of ulcerative colitis (UC) through regulating the intestinal homeostasis. Several inflammatory diseases are reported to be regulated by proline-rich tyrosine kinase 2B (PTK2B). However, the role of PTK2B in regulating the function of neutrophils and the pathogenesis of UC remains unknown. In this study, the mRNA and protein levels of PTK2B in the colonic tissues from UC patients were measured by using quantitative real-time polymerase chain reaction (qRT-PCR), western blotting, and immunohistochemistry. TAE226, a PTK2B inhibitor, was used to inhibit the activity of PTK2B in neutrophils, and then, the pro-inflammatory factors were analyzed by using qRT-PCR and ELISA. To determine the role of PTK2B in intestinal inflammation, a dextran sulfate sodium (DSS)-induced colitis model was established in PTK2B gene knockout (PTK2B KO) and wild-type (WT) mice. We found that compared with healthy donor controls, the expression level of PTK2B was significantly elevated in inflamed mucosa from UC patients. In addition, expression of PTK2B was positively correlated with the severity of disease. Pharmacological inhibition of PTK2B could markedly reduce the generation of reactive oxygen species (ROS), myeloperoxidase (MPO), and antimicrobial peptides (S100a8 and S100a9) in neutrophils. The vitro study showed that tumor necrosis factor (TNF)-α is involved in promoting the expression of PTK2B in neutrophils. As expected, UC patients treated with infliximab, an anti-TNF-α agent, showed significantly reduced level of PTK2B in neutrophils, as well as in the intestinal mucosa. Of note, compared with DSS-treated WT mice, DSS-treated PTK2B KO mice showed more severe colitis symptoms. Mechanistically, PTK2B could enhance neutrophil migration by regulating CXCR2 and GRK2 expression via the p38 MAPK pathway. Additionally, mice treated with TAE226 exhibited the same effects. In conclusion, PTK2B is involved in the pathogenesis of UC by promoting the migration of neutrophils and inhibiting mucosal inflammation, highlighting PTK2B as a new potential therapeutic target to treat UC.


Assuntos
Colite Ulcerativa , Quinase 2 de Adesão Focal , Animais , Camundongos , Colite Ulcerativa/metabolismo , Colo/metabolismo , Sulfato de Dextrana/toxicidade , Modelos Animais de Doenças , Quinase 2 de Adesão Focal/genética , Quinase 2 de Adesão Focal/metabolismo , Imunidade , Inflamação/metabolismo , Mucosa Intestinal/metabolismo , Neutrófilos/metabolismo , Inibidores do Fator de Necrose Tumoral/uso terapêutico , Fator de Necrose Tumoral alfa/metabolismo , Humanos
4.
Langmuir ; 40(24): 12465-12474, 2024 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-38855944

RESUMO

Polymerization confined to the pore was first adapted for the nanoscale structure adjustment of adsorption resin. The self-cross-linked polymer (P-1) formed in the pore of hyper-cross-linked resin (HR) by the Friedel-Crafts reaction of p-dichloroxylene (p-DCX), occupying the macropore of the HR resin and bringing about an external micropore. Compared with the raw HR resin, the volume of the micropore of HR@P-1 in 0.4 < D < 1 nm increased but the volume of the macropore has obviously decreased. After the loading of P-1 in the nanopore of HR, HR@P-1 has better gas adsorption performance. At 298 and 100 KPa, the adsorption capacity of CO2 is almost 30% higher than that of HR, reaching 35.7 cm3/g, due to the increase in the smaller micropore volume. Moreover, HR@P-1 has also been found to be the first C2H6-selective adsorption resin. The uptake of C2H6 is up to 56 cm3/g, and the IAST selectivity of C2H6/CH4 reaches 15.3. HR@P-1 can also separate syngas efficiently at ambient temperature and be regenerated by simple vacuum operation.

5.
Org Biomol Chem ; 22(36): 7500-7517, 2024 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-39189805

RESUMO

Axially chiral C2-arylquinoline has been successfully constructed via asymmetric heteroannulation of alkynes catalyzed by chiral phosphoric acid with high yield and high enantioselectivity. Inspired by this intriguing work, theoretical calculations have been carried out, and the detailed reaction mechanism has been elaborated, in which the whole reaction can be divided into steps including hydrogen transfer, C-N bonding, annulation reaction and the final dehydration processes. The initial hydrogen-transfer reaction has two possible pathways, while the subsequent C-N bonding process has eight possible pathways. Then, after the annulation reaction and the final dehydration processes, the major product and byproduct were formed. QTAIM and IGMH analyses were used to illustrate the role of weak intermolecular interactions in the catalytic process, and the distortion/interaction and EDA analyses provided a deeper understanding of the origin of enantioselectivity. The calculated results are consistent with the experimental results. This work would provide valuable insights into asymmetric reactions catalyzed by chiral phosphoric acid.

6.
BMC Infect Dis ; 24(1): 677, 2024 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-38977947

RESUMO

BACKGROUND: Hoylesella marshii can be isolated from human oral cavities affected by dental pulp and periodontal infections, as well as from the dental plaque of healthy individuals, making it a common bacterium within the oral microbiota. However, its role in causing pleural infections in humans is rare. CASE PRESENTATION: A case of purulent pleural effusion occurred shortly after discharge in an elderly patient who had undergone surgery for gastric cancer. The infection was identified as being caused by an obligate anaerobe through laboratory culture, and was further identified as Hoylesella marshii causing pleural infection through 16 S rRNA gene sequence analysis. Susceptibility testing guided precise treatment with cefoperazone-sulbactam and metronidazole. The patient's clinical symptoms improved rapidly, laboratory test indicators gradually returned to normal, and the patient ultimately recovered. CONCLUSION: Hoylesella marshii can cause pleural infections in humans. Clinical microbiology laboratories should pay special attention to the cultivation of obligate anaerobes when routine aerobic cultures do not show bacterial growth but bacteria are visible on smear staining, and when conventional identification methods fail to identify the bacterium, analysis based on the highly conserved 16 S rRNA gene sequence can accurately and specifically identify the bacterium, guiding clinicians in formulating precise anti-infection strategies.


Assuntos
Antibacterianos , RNA Ribossômico 16S , Humanos , RNA Ribossômico 16S/genética , Antibacterianos/uso terapêutico , Masculino , Idoso , Derrame Pleural/microbiologia , Testes de Sensibilidade Microbiana , Metronidazol/uso terapêutico , Doenças Pleurais/microbiologia , Doenças Pleurais/diagnóstico
7.
Phys Chem Chem Phys ; 26(8): 7137-7148, 2024 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-38348666

RESUMO

The ONIOM (ωb97xd/6-31G(d,p):pm6) method was used to study the reaction mechanism of dimethylcyclopentane to toluene by the [GaH]2+ active site of Ga-ZSM-5. The results showed that the rate-determining step in the dimethylcyclopentane aromatization process is the ring expansion process. Compared to those of methylcyclopentane to benzene (D. D. Zhang, H. Y. Liu, L. X. Ling, H. R. Zhang, R. G. Zhang, P. Liu and B. J. Wang, Phys. Chem. Chem. Phys., 2021, 23, 10988-11003.), the free energy barriers of dimethylcyclopentane to toluene are significantly decreased, indicating that toluene is easier to produce than benzene, which confirmed the experimental results that a higher proportion of toluene than benzene is produced in the MTA process.

8.
BMC Cardiovasc Disord ; 24(1): 539, 2024 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-39379795

RESUMO

BACKGROUND: The Oxidative Balance Score (OBS) was created to evaluate an individual's overall antioxidant status. The objective of this study was to examine the association between OBS and abdominal aortic calcification (AAC) among individuals aged ≥ 40 years. METHODS: This population-based cross-sectional study used data from the National Health and Nutrition Examination Survey in 2013-2014 and included adults aged ≥ 40 years. Survey-weighted multivariable logistic and restricted cubic spline models were used to assess the association between OBS and AAC. RESULTS: Among 2520 participants, 744 were diagnosed with AAC (weighted percentage, 28.13%). Survey-weighted multivariable logistic revealed an inverse association between OBS and AAC [0.98 (0.96, 1.00)], and the nonlinear dose-response relationship was observed. Subgroup analysis and interaction tests revealed that this inverse relationship was consistent across different populations (all P for interaction > 0.05). CONCLUSIONS: OBS was inversely associated with the prevalence of AAC among individuals aged ≥ 40 years. Maintaining a higher OBS may be beneficial in reducing the burden of AAC.


Assuntos
Aorta Abdominal , Doenças da Aorta , Inquéritos Nutricionais , Estresse Oxidativo , Calcificação Vascular , Humanos , Estudos Transversais , Masculino , Feminino , Calcificação Vascular/diagnóstico por imagem , Calcificação Vascular/epidemiologia , Pessoa de Meia-Idade , Aorta Abdominal/diagnóstico por imagem , Aorta Abdominal/patologia , Idoso , Doenças da Aorta/epidemiologia , Doenças da Aorta/diagnóstico por imagem , Prevalência , Fatores Etários , Fatores de Risco , Estados Unidos/epidemiologia , Adulto , Medição de Risco , Antioxidantes/metabolismo
9.
Biochem Genet ; 2024 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-38411943

RESUMO

Non-small cell lung cancer (NSCLC) patients are characterized by distant metastasis and poor prognosis. Growing evidence has implied that circular RNAs (circRNAs) are involved in multiple tumor progression, including NSCLC. The objective of the present study was to functionally dissect the role and mechanism of circ_BLNK in NSCLC development and progression. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of circ_BLNK, miR-942-5p, and forkhead box protein O1 (FOXO1) in NSCLC tissues and cells. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, 5-ethynyl-2'-deoxyuridine (EdU) assay and colony formation assay detected cell proliferation; the protein expression levels were tested by western blot assay; cell apoptosis was measured by flow cytometry, and transwell assay detected cell migration and invasion. The molecular targeting relationship was determined by dual-luciferase reporter assay. The effect of circ_BLNK overexpression on tumor growth was detected by in vivo experiments and immunohistochemistry. Circ_BLNK was dramatically decreased in NSCLC, and overexpression of circ_BLNK inhibited proliferation, migration, and invasion of NSCLC cells and promoted cell apoptosis. Circ_BLNK level was negatively correlated with miR-942-5p expression and positively correlated with FOXO1 expression. Moreover, circ_BLNK acted as a sponge for miR-942-5p, which targeted FOXO1. Rescue assays presented that miR-942-5p reversed the anticancer action of circ_BLNK in NSCLC. Besides that, miR-942-5p inhibition suppressed the oncogenic behaviors, which were attenuated by FOXO1 knockdown. Animal experiments exhibited that circ_BLNK upregulation repressed tumor growth in vivo. Our study demonstrated a novel regulatory mechanism that circ_BLNK/miR-942-5p/FOXO1 axis adjusted non-small cell lung cancer development.

10.
Mol Cell Biochem ; 2023 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-37659034

RESUMO

N6-methyladenosine (m6A) modification is a class of epitope modifications that has received significant attention in recent years, particularly in relation to its role in various diseases, including sepsis. Epigenetic research has increasingly focused on m6A modifications, which is influenced by the dynamic regulation of three protein types: ?Writers" (such as METTL3/METTL14/WTAP)-responsible for m6A modification; ?Erasers" (FTO and ALKBH5)-involved in m6A de-modification; and ?Readers" (YTHDC1/2, YTHDF1/2/3)-responsible for m6A recognition. Sepsis, a severe and fatal infectious disease, has garnered attention regarding the crucial effect of m6A modifications on its development. In this review, we attempted to summarize the recent studies on the involvement of m6A and its regulators in sepsis, as well as the significance of m6A modifications and their regulators in the development of novel drugs and clinical treatment. The potential value of m6A modifications and modulators in the diagnosis, treatment, and prognosis of sepsis has also been discussed.

11.
Biotechnol Lett ; 45(5-6): 619-628, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37071384

RESUMO

OBJECTIVES: Eucommia ulmoides gum (EUG) is an important natural biomass rubber material, which is usually extracted from Eucommia ulmoides Oliver (EUO). In the extraction process of EUG, pretreatment is the most important step which can efficiently damage EUG-containing cell wall and improve yield of EUG. RESULTS: The FT-IR, XRD, DSC and TG results showed that the thermal properties and structure of the EUG from the dilute acids hydrolysis residue are similar with that of the EUG directly extracted from EUO leaves (EUGD). EUO leaves hydrolysis with AA had the highest EUG yield (16.1%), which was higher than the EUGD yield (9.5%). In the case of the EUO leaves hydrolysis with 0.33 ~ 0.67 wt% of acetic acid (AA), the total sugar was stable in the range of 26.82-27.67 g/L. Furthermore, the EUO leaves acid hydrolysate (AA as reagent) was used as carbon sources for lipid-producing fermentation by Rhodosporidium toruloides. After 120 h of fermentation, the biomass, lipid content and lipid yield were 12.13 g/L, 30.16% and 3.64 g/L, respectively. The fermentation results indicated organic acids were no toxic for Rhodosporidium toruloides and the AA also could be used as carbon source for fermentation.


Assuntos
Eucommiaceae , Hidrólise , Eucommiaceae/química , Espectroscopia de Infravermelho com Transformada de Fourier , Ácido Acético , Folhas de Planta/química , Carbono/análise
12.
Mikrochim Acta ; 191(1): 1, 2023 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-38040940

RESUMO

A polyvinylpyrrolidone-capped (PVP-capped) strategy is reported to synthesize Ag NPs on silicon wafers via galvanic replacement reaction for SERS detection of adenine, where PVP acts as stabilizing agent in synthesis and efficient enrichment in detection. The morphologies of Ag NPs are optimized with uniform particle size by adjusting synthesis conditions, which hold excellent SERS performances like a high enhancement factor of 1.42 × 106, good uniform, reproducibility, and transferable nature. With the protection of the capped PVP, the Ag NPs keep excellent SERS properties even against harsh conditions of high temperature (100 ℃) and strong acid and base for 24 h. Utilizing the structural feature of PVP with abundant carbonyl groups, the PVP-capped Ag NPs achieve efficient enrichment of adenine through hydrogen bonding and π-interactions, which is analyzed by density functional theory. Quantitative detection of adenine is performed with a wide linear range from 10-4 to 10-8 M and a low limit of detection of 1 nM. Detection of adenine in human urine samples is achieved with a recovery of 99.1-103.4% and an RSD of less than 5%.

13.
J Environ Sci (China) ; 132: 109-121, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37336601

RESUMO

Long-term stereoscopic observations of aerosol, NO2, and HCHO were carried out at the Yangmeikeng (YMK) site in Shenzhen. Aerosol optical depths and NO2 vertical column concentration (NO2 VCD) derived from MAX-DOAS were found to be consistent with other datasets. The total NO2 VCD values of the site remained low, varying from 2 × 1015 to 8 × 1015 mol/cm2, while the HCHO VCD was higher than NO2 VCD, varying from 7 × 1015 to 11 × 1015 mol/cm2. HCHO VCD was higher from September to early November than that was from mid-late November to December and during February 2021, in contrast, NO2 VCD did not change much during the same period. In January, NO2 VCD and HCHO VCD were both fluctuating drastically. High temperature and HCHO level in the YMK site is not only driving the ozone production up but also may be driving up the ozone concentration as well, and the O3 production regime in the YMK site tends to be NOx-limited. At various altitudes, backward trajectory clustering analysis and Potential Source Contribution Function (PSCF) were utilized to identify possible NO2 and HCHO source locations. The results suggested that the Huizhou-Shanwei border and the Daya Bay Sea area were the key potential source locations in the lower (200 m) and middle (500 m) atmosphere (WPSCF > 0.6). The WPSCF value was high at the 1000 m altitude which was closer to the YMK site than the near ground, indicating that the pollution transport capability in the upper atmosphere was limited.


Assuntos
Poluentes Atmosféricos , Ozônio , Ozônio/análise , Poluentes Atmosféricos/análise , Monitoramento Ambiental/métodos , Dióxido de Nitrogênio/análise , Poluição Ambiental/análise
14.
Arch Virol ; 167(8): 1713-1715, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35606464

RESUMO

Here, we report the genomic characterization of two siphophages, named hairong and ZY21, that infect the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae. The genome sequences of hairong (112,842 bp) and ZY21 (112,006 bp) were determined. Global sequence comparison showed that hairong, ZY21, and two phages of the genus Nickievirus (nickie and psageB1) are similar but are not closely related to any other known phage, and they comprise a unique phylogenetic cluster. Moreover, hairong represents a new genus related to Nickievirus. Comparative genomic analysis revealed some common features shared by the four nickie-like phages.


Assuntos
Actinidia , Bacteriófagos , Siphoviridae , Genômica , Filogenia , Doenças das Plantas , Pseudomonas syringae/genética
15.
Int J Med Sci ; 19(11): 1680-1694, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36237989

RESUMO

Gastric cancer is a highly malignant tumor. Gastric cancer stem cells (GCSCs) are the main causes of drug resistance, metastasis, recurrence, and poor prognosis. As a secondary metabolite of lichen, Atranorin has a variety of biological effects, such as antibacterial, anti-inflammatory, analgesic, and wound healing; however, its killing effect on GCSCs has not been reported. In this study, we constructed Atranorin complexes comprising superparamagnetic iron oxide nanoparticles (SPION) (Atranorin@SPION). In vitro and in vivo experiments confirmed that Atranorin@SPION could significantly inhibit the proliferation, invasion, angiogenesis, and tumorigenicity of CD44+/ CD24+ GCSCs, and induce oxidative stress injury, Fe2+ accumulation, and ferroptosis. Quantitative real-time reverse transcription PCR and western blotting results showed that Atranorin@SPION not only reduced the expression levels of GCSC stem cell markers and cell proliferation and division markers, but also significantly inhibited the expression levels of key molecules in the cystine/glutamate transporter (Xc-)/glutathione peroxidase 4 (GPX4) and Tet methylcytosine dioxygenase (TET) family proteins. The results of high performance liquid chromatography-mass spectrometry and Dot blotting showed that Atranorin@SPION significantly inhibited the mRNA 5­hydroxymethylcytidine modification of GCSCs. Meanwhile, the results of RNA immunoprecipitation-PCR also indicated that Atranorin@SPIONs significantly reduced the 5-hydroxymethylcytidine modification level of GPX4 and SLC7A11 mRNA 3' untranslated region in GCSCs, resulting in a decrease in their stability, shortening their half-lives and reducing translation activity. Therefore, this study revealed that Atranorin@SPIONs induced ferroptosis of GCSCs by weakening the expression of the Xc-/GPX4 axis and the 5-hydroxymethylcytidine modification of mRNAs in the pathway, thereby achieving their therapeutic effect on gastric cancer.


Assuntos
Dioxigenases , Ferroptose , Neoplasias Gástricas , Regiões 3' não Traduzidas , Sistema X-AG de Transporte de Aminoácidos/genética , Sistema X-AG de Transporte de Aminoácidos/metabolismo , Sistema X-AG de Transporte de Aminoácidos/farmacologia , Analgésicos/uso terapêutico , Antibacterianos/uso terapêutico , Anti-Inflamatórios/farmacologia , Linhagem Celular Tumoral , Cistina/genética , Cistina/metabolismo , Cistina/farmacologia , Citidina/análogos & derivados , Dioxigenases/genética , Dioxigenases/metabolismo , Dioxigenases/farmacologia , Ferroptose/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Hidroxibenzoatos , Nanopartículas Magnéticas de Óxido de Ferro , Células-Tronco Neoplásicas/patologia , Fosfolipídeo Hidroperóxido Glutationa Peroxidase , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia
16.
Biochem Biophys Res Commun ; 557: 228-235, 2021 06 11.
Artigo em Inglês | MEDLINE | ID: mdl-33887588

RESUMO

LncRNA-cCSC1 is highly expressed in colorectal cancer (CRC). The study was designed to evaluate the function and mechanism of lncRNA-cCSC1 in cell proliferation of CRC. RT-PCR was used to measure the expression levels of lncRNA-cCSC1 in CRC cell lines. CCK-8, colony formation, EdU staining, flow cytometry and Western blot were performed to examine the effect of interference with lncRNA-cCSC1 expression on cell proliferation. miR-124-3p and the target genes of miR-124-3p were investigated using bioinformatics analysis and verified by dual-luciferase reporter, RT-PCR and Western blot. Rescue experiments were carried out to confirm the role of miR-124-3p in cell proliferation of CRC. Our results showed that cell proliferation of CRC was promoted by lncRNA-cCSC1 upregulation and inhibited by lncRNA-cCSC1 downregulation. In addition, miR-124-3p is predicted to be the target of lncRNA-cCSC1 and is negatively correlated with lncRNA-cCSC1. Moreover, the addition of miR-124-3p mimics or inhibitor reversed the effects induced by lncRNA-cCSC1 overexpression or silencing on cell proliferation of CRC. Additionally, lncRNA-cCSC1 regulated the expression level of CD44, a target gene of miR-124-3p. Finally, we studied the effects of the lncRNA-cCSC1/miR-124-3p axis on CD44. These results indicate that lncRNA-cCSC1 promotes cell proliferation of CRC through sponging miR-124-3p and upregulating CD44.


Assuntos
Adenocarcinoma/metabolismo , Proliferação de Células/genética , Neoplasias Colorretais/metabolismo , Receptores de Hialuronatos/metabolismo , MicroRNAs/metabolismo , RNA Longo não Codificante/metabolismo , Adenocarcinoma/genética , Adenocarcinoma/patologia , Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia , Bases de Dados Genéticas , Regulação para Baixo , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Receptores de Hialuronatos/genética , MicroRNAs/genética , RNA Longo não Codificante/genética , Regulação para Cima
17.
Phys Chem Chem Phys ; 23(18): 10988-11003, 2021 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-33929467

RESUMO

Naphthenes are key intermediates in the formation of aromatic compounds during the methanol to aromatics (MTA) reaction, and the dehydrogenation process is more important than the hydrogen transfer process. Theoretical studies were performed to investigate the methylcyclopentane, which represents a naphthene, to benzene MTA process catalyzed by ZSM-5 before and after introducing Ga, showing that Ga-ZSM-5 was more favorable for carrying out the reaction than two H-type ZSM-5 (H-Z1 and H-Z2) models. H-Z1 and H-Z2 are favorable for the transfer of H during ring expansion reactions and the reformation of Brønsted acids, but the dehydrogenation reactions involving H-Z1 and H-Z2 require high free-energy barriers to be overcome. Although introducing Ga to ZSM-5 is not conducive to the transfer of H after dehydrogenation, it can reduce the extremely high dehydrogenation free-energy barrier compared with H-Z1 and H-Z2; this is mainly because Ga at dehydrogenation active centers, [GaH]2+, can accept electrons and donate them to the H atoms of [GaH]2+, giving H negative charge and making it easy to combine with positive B-acid H atoms that come from methylcyclopentane, cyclohexene, and cyclohexadiene to produce H2. Also, analysis of the transition state structures of all DH processes shows that Ga-ZSM-5 is more favorable for promoting the combination of H to produce H2 than H-Z1 and H-Z2.

18.
Support Care Cancer ; 29(10): 5967-5972, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33765206

RESUMO

PURPOSE: To explore whether anxiety and depression are prognostic indexes for overall survival in patients with nasopharyngeal carcinoma (NPC) who underwent intensity-modulated radiotherapy (IMRT). METHODS: Clinical data were collected for NPC patients who underwent IMRT. Anxiety and depression were investigated before radiotherapy by using the Hospital Anxiety and Depression Scale (HADS). The survival rate was calculated by the Kaplan-Meier method, and survival curves were compared among patients with different levels of anxiety and depression. The Cox risk regression model was used to screen the factors affecting survival. RESULTS: A total of 390 initially treated NPC patients were included in the study. Among them, 166 patients suffered from anxiety, and 95 patients suffered from depression before radiotherapy. The 5-year overall survival rates for patients with and without anxiety before radiotherapy were 71.6% and 81.8% (χ2 = 5.31, P = 0.021), respectively. The 5-year overall survival rates for patients with and without depression before radiotherapy were 74.3% and 78.1% (χ2 = 0.05, P = 0.82), respectively. Cox regression analysis indicated clinical stages (HR = 3.982, 95% CI: 2.365~6.705), anxiety (HR = 1.832, 95% CI: 1.140~2.944), and gender (HR = 0.555, 95% CI: 0.313~0.984) as independent prognostic factors. CONCLUSION: Anxiety before radiotherapy is associated with poor prognosis in NPC patients.


Assuntos
Neoplasias Nasofaríngeas , Radioterapia de Intensidade Modulada , Humanos , Carcinoma Nasofaríngeo/radioterapia , Neoplasias Nasofaríngeas/patologia , Neoplasias Nasofaríngeas/radioterapia , Estadiamento de Neoplasias , Prognóstico , Estudos Retrospectivos
19.
Int J Med Sci ; 18(4): 921-928, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33456349

RESUMO

Background: Although COVID-19 pneumonia is spreading internationally, knowledge regarding the factors associated with the illness severity of patients remains limited. We aimed to identify the factors associated with the disease severity of patients with COVID-19 pneumonia induced by a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: We prospectively enrolled a single-center case series of adult patients with COVID-19 admitted to the Infectious Disease Hospital of Jining, Jining City, Shandong Province, China, from January 24 to March 1, 2020. Demographics, clinical characteristics, and laboratory findings were compared to investigate the risk factors related with the disease severity of COVID-19 pneumonia patients. Results: We included a total of 78 patients with COVID-19 pneumonia, of whom 6 had the severe type. As compared to a moderately ill cohort, our analysis showed that shortness of breath, fatigue, longer days from illness onset to diagnosis confirmed, neutrophil percentages > 70%, neutrophil counts > 6.3 × 109/L, lymphocyte percentages < 20%, lymphocyte counts < 1.0 × 109/L, platelet < 100 × 109/L, C-reactive protein (CRP) > 10 mg/L, neutrophil to platelet ratio (NPR) > 2.3, neutrophil to lymphocyte ratio (NLR) > 3.9, aspartate aminotransferase (AST) > 40 U/L, albumin < 40 g/L, lactate dehydrogenase (LDH) > 245 U/L, and glucose > 6.1 mmol/L were predictors of disease severity in COVID-19 pneumonia. In the sex-, age-, and comorbid illness-matched case-control study, neutrophil percentages > 70%, neutrophil counts > 6.3 × 109/L, lymphocyte percentages < 20%, NPR > 2.3, NLR > 3.9, albumin < 40 g/L, and LDH > 245 U/L remained associated with the early detection and identification of severe patients. Conclusion: We demonstrated that neutrophil percentages > 70%, neutrophil counts > 6.3 × 109/L, lymphocyte percentages < 20%, NPR > 2.3, NLR > 3.9, albumin < 40 g/L, and LDH > 245 U/L might predict the severity of illness in patients with COVID-19 pneumonia.


Assuntos
COVID-19/sangue , COVID-19/epidemiologia , Adulto , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , SARS-CoV-2 , Índice de Gravidade de Doença
20.
Mar Drugs ; 19(4)2021 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-33806230

RESUMO

Macrolides are a significant family of natural products with diverse structures and bioactivities. Considerable effort has been made in recent decades to isolate additional macrolides and characterize their chemical and bioactive properties. The majority of macrolides are obtained from marine organisms, including sponges, marine microorganisms and zooplankton, cnidarians, mollusks, red algae, bryozoans, and tunicates. Sponges, fungi and dinoflagellates are the main producers of macrolides. Marine macrolides possess a wide range of bioactive properties including cytotoxic, antibacterial, antifungal, antimitotic, antiviral, and other activities. Cytotoxicity is their most significant property, highlighting that marine macrolides still encompass many potential antitumor drug leads. This extensive review details the chemical and biological diversity of 505 macrolides derived from marine organisms which have been reported from 1990 to 2020.


Assuntos
Anti-Infecciosos/farmacologia , Antineoplásicos/farmacologia , Organismos Aquáticos/metabolismo , Macrolídeos/farmacologia , Animais , Anti-Infecciosos/isolamento & purificação , Antineoplásicos/isolamento & purificação , Humanos , Macrolídeos/isolamento & purificação , Estrutura Molecular , Relação Estrutura-Atividade
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