A long non-coding RNA lnc210 promotes adipogenic differentiation of buffalo intramuscular adipocytes.

Intramuscular fat (IMF) content is one of the most significant factors influencing beef quality in terms of tenderness, flavor, and juiciness. Thus, internal factors affecting IMF deposition have received considerable attention for decades. In this study, we demonstrated a long non-coding RNA, lnc210, promoted adipogenic differentiation of buffalo intramuscular adipocytes. lnc210 was rich in adipose tissue and showed increased expression with the adipogenic differentiation of buffalo intramuscular adipocytes. lnc210 was mainly expressed in the nucleus of adipocytes. Full-length lnc210 was obtained by rapid amplification of cDNA ends technology. lnc210 overexpression promoted lipid accumulation by upregulating the mRNA expression of peroxisome proliferator-activated receptor gamma (PPARG) and CCAAT enhancer binding protein alpha (C/EBPα) in buffalo intramuscular adipocytes. These results provide a basis for an in-depth analysis of the role of lnc210 in accelerating IMF deposition in buffaloes.

Psychometric Validation of the Edmonton Symptom Assessment System in Chinese Patients.

CONTEXT: The Edmonton Symptom Assessment System is a simple and widely used questionnaire to assess patient symptoms. OBJECTIVES: We aimed to develop and verify a Chinese version of the Edmonton Symptom Assessment System (C-ESAS) for use with Chinese patients. METHODS: Two hundred ten patients were surveyed in the Department of Gastroenterology of Xinqiao hospital in southwest China. Patients completed the C-ESAS and the Chinese version of the M. D. Anderson Symptom Inventory to assess internal consistency, test-retest reliability, and concurrent validity of the C-ESAS. The questionnaires were completed again two hours later. RESULTS: The C-ESAS demonstrated acceptable internal consistency (Cronbach α = 0.72). This indicated that no question had an inappropriate effect on the score. Pearson correlation coefficients for the C-ESAS symptom scores between baseline and after two hours ranged from 0.47 (95% CI, 0.35-0.89) to 0.92 (95% CI, 0.57-0.94), indicating strong test-retest reliability. The corresponding Chinese version of the M. D. Anderson Symptom Inventory symptom scores ranged from 0.66 (95% CI, 0.58-0.95) to 0.96 (95% CI, 0.73-0.97), indicating good concurrent validity. The average time to complete the C-ESAS differed according to patient education level. CONCLUSION: The C-ESAS is a good tool for measuring multidimensional symptoms in Chinese patients.