A new isolate of Streptomyces lateritius (Z1-26) with antibacterial activity against fish pathogens and immune enhancement effects on crucian carp (Carassius auratus).

The Streptomyces lateritius Z1-26 was isolated from soil samples which showed broad-spectrum antibacterial activity against a broad range of fish pathogens. The In Vivo Imaging System (IVIS) monitored that strain Z1-26 could survive and colonize in the gills and abdomen of crucian carp. The effects of dietary supplementation with strain Z1-26 were evaluated with respect to the growth performance, antioxidant capacity, and immune response of crucian carp. The results showed that the Z1-26-fed fish had a significantly higher growth rate than the fish fed the control diet. The immune and antioxidant parameters revealed that the non-specific immune indicators (AKP, SOD, and LZM) of the serum, the expression of immune-related genes (IgM, C3, and LZM), and antioxidant-related genes (Nrf2 and Keap1) of the immune organs were significantly increased, whereas the expression of pro-inflammatory factors (IL-1ß, IL-8, and TNF-α) of the immune organs was significantly down-regulated in crucian carp fed strain Z1-26 compared with fish fed a control diet. Moreover, fish fed with Z1-26 supplemented diets showed a significantly improved survival rate after Aeromonas hydrophila infection. In addition, the whole genome analysis showed that strain Z1-26 possesses 28 gene clusters, including 6 polyketide synthetase (PKS), 4 non-ribosomal peptide-synthetase (NRPS), 1 bacteriocin, and 1 lantipeptide. In summary, these results indicated that strain Z1-26 could improve the growth performance and disease resistance in crucian carp, and has the potential to be developed as a candidate probiotics for the control of bacterial diseases in aquaculture.

Effects of M. oleifera leaf extract on the growth, physiological response and related immune gene expression of crucian carp fingerlings under Aeromonas hydrophila infection.

We evaluated the effect of dietary supplementation with Moringa oleifera leaf extract on the resistance to Aeromonas hydrophila infection in crucian carp. The fish were randomly divided into five groups: the basal diet, the basal diet supplied with 0.25% (0.25 M), 0.5% (0.5 M), 0.75% (0.75 M) and 1.0% M. oleifera leaf extract (1.0 M) for 8 weeks. The growth, antioxidant capabilities, related immune genes as well as resistance to A. hydrophila infection were determined. The results showed that compared with the control group, the weight gain, specific growth rate in the group of 0.5% M. oleifera leaf extract, serum superoxide dismutase (SOD), albumin (ALB) and glutathione peroxidase (GSH-Px), the gene expression of hepatopancreas BTB and CNC homolog 1 (Bach1), NF-E2-related factor 2 (Nrf2), peroxidases (PRX) and NADPH oxidase (NOX) in the group of 0.5%-1.0% M. oleifera leaf extract increased, while feed conversion ratio, serum cortisol, red blood cell (RBC), alanine aminotransferase (ALT), malonaldehyde (MDA) decreased in the group of 0.5%-1.0% M. oleifera leaf extract before the stress. After the infection, the group of 0.5% or 0.75% M. oleifera leaf extract also could improve the serum ALB, hepatopancreas Kelch-like-ECH-associated protein 1 (Keap1), Bach1, Nrf2, TOR, PRX and NOX and reduce cortisol compared with the control group. In summary, this study suggested that 0.5% M. oleifera leaf extract inclusion increased the growth performance, even had positive effects on physiological and immune function, and enhanced resistance against pathogenic infections in crucian carp. The optimum level of M. oleifera leaf extract for crucian carp was estimated to be 0.35%-0.48% based on polynomial comparison with FCR and SGR.

Effects of high fat in the diet on growth, antioxidant, immunity and fat deposition of Macrobrachium rosenbergii post-larvae.

Lipids are essential nutrients for organisms, and high-fat feeds for shrimp may cause oxidative stress. This study evaluated the effects of feeding high fat in the diet on the growth, antioxidant, immunity, and liver fat accumulation of Macrobrachium rosenbergii post-larvae. Five groups with an initial body weight of 0.0084 ± 0.001 g were fed five isonitrogenous and isoenergetic diets (47.01% crude protein and 18.40 kJ/g gross energy) containing 8%, 10%, 12%, 14% and 16% (named L8, L10, L12, L14 and L16) lipid for 8 weeks, respectively. The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of L8 group were significantly higher than those of L10, L12, L14 and L16 group (P < 0.05), and the feed coefficient (FCR) of L8 group was significantly lower than that of other groups (P < 0.05). With the increase of dietary fat level, the content of MDA and the activity of SOD increased significantly, and the activities of T-AOC and CAT decreased significantly (P < 0.05). H&E staining clearly revealed the occurrence of hepatocyte swelling, hepatocyte vacuolization and nucleus displacement to the peripheral cell vacuolization in the L16 group, and hepatic lipid accumulation was further observed in the L14 and L16 group by Oil red O staining. In addition, high-fat diet significantly upregulated the expression of Dorsal, Relish and IκBα mRNA, and also upregulated the expression of fat synthesis-related genes FAS, ACC, DGAT and fat transport-related gene FABP (P < 0.05), and significantly downregulated the expression of fat metabolism-related genes AMPK and CPT-1 (P < 0.05) compared to that of the L8 group. In conclusion, this study showed that feeding a high-fat diet could induce oxidative stress, inhibit growth performance, alter antioxidant capacity, cause hepatic fat deposition and affect the immune system of M. rosenbergii post-larvae.