Einstein-Stokes relation for small bubbles at the nanoscale.

As the physicochemical properties of ultrafine bubble systems are governed by their size, it is crucial to determine the size and distribution of such bubble systems. At present, the size or size distribution of nanometer-sized bubbles in suspension is often measured by either dynamic light scattering or the nanoparticle tracking analysis. Both techniques determine the bubble size via the Einstein-Stokes equation based on the theory of the Brownian motion. However, it is not yet clear to which extent the Einstein-Stokes equation is applicable for such ultrafine bubbles. In this work, using atomic molecular dynamics simulation, we evaluate the applicability of the Einstein-Stokes equation for gas nanobubbles with a diameter less than 10 nm, and for a comparative analysis, both vacuum nanobubbles and copper nanoparticles are also considered. The simulation results demonstrate that the diffusion coefficient for rigid nanoparticles in water is found to be highly consistent with the Einstein-Stokes equation, with slight deviation only found for nanoparticle with a radius less than 1 nm. For nanobubbles, including both methane and vacuum nanobubbles, however, large deviation from the Einstein-Stokes equation is found for the bubble radius larger than 3 nm. The deviation is attributed to the deformability of large nanobubbles that leads to a cushioning effect for collision-induced bubble diffusion.

LncRNA MIR155HG functions as a ceRNA of miR-223-3p to promote cell pyroptosis in human degenerative NP cells.

Nucleus pulposus (NP) cell pyroptosis plays a critical role in the pathogenesis of intervertebral disk degeneration (IDD). MIR155 host gene (MIR155HG) is a long non-coding RNA with pro-inflammatory activity. However, very little is known about its role in NP cell pyroptosis. This study aimed to observe the impact of MIR155HG on cell pyroptosis and to explore the underlying mechanism in human degenerative NP cells. Our results demonstrated that MIR155HG expression was significantly increased in human degenerative NP tissue samples and showed a positive correlation with Pfirrmann score. Overexpression of MIR155HG through a lentiviral vector decreased miR-223-3p levels, up-regulated NLRP3 expression and induced cell pyroptosis in human degenerative NP cells. A ceRNA action mode was identified among MIR155HG, miR-223-3p, and NLRP3. The stimulatory effect of MIR155HG on human degenerative NP cell pyroptosis was significantly reversed by pretreatment with miR-223-3p mimic or NLRP3 siRNA. In summary, these data suggest that MIR155HG sponges miR-223-3p to promote NLRP3 expression, leading to induction of cell pyroptosis in human degenerative NP cells. Targeting MIR155HG could be a novel and promising strategy to slow down the progression of IDD.

Ratiometric fluorometric determination of the anthrax biomarker 2,6-dipicolinic acid by using europium(III)-doped carbon dots in a test stripe.

Europium(III)-doped carbon dots (Eu-CDs) were prepared from citric acid and europium nitrate via a one-pot pyrolytic method. The Eu-CDs emit intense blue fluorescence (with excitation/emission peaks at 365/465 nm), are water soluble and biocompatible. On addition of 2,6-dipicolinic acid (DPA; an anthrax biomarker), ligand-to-ion energy transfer occurs from DPA to Eu(III) which has a red emission peaking at 615 nm. This results in an increase of the intensity of the red fluorescence. DPA can be detected by the ratio of fluorescence intensities at 616 and 475 nm. The method has an analytical range that extends from 5 to 700 nmol·L-1, with a 5 nmol·L-1 detection limit. The Eu-CDs also were incorporated into a test paper for visual detection of DPA with a portable UV lamp and a smartphone. In this case, the detection limit is 1 µmol·L-1. The Eu-CDs internalize well into HeLa cells, and this paves the way to bioimaging. Graphical abstract Schematic of a method for visual detection of 2,6-dipicolinic acid (DPA, an anthrax biomarker) by using a test stripe impregnated with europium(III)-doped carbon dots (Eu-CDs).

Degradation Mechanism of Micro-Nanobubble Technology for Organic Pollutants in Aqueous Solutions.

Micro-nanobubbles (MNBs) technology has emerged as an effective means of sewage treatment, while the molecular mechanism for its pollutant degradation is still unknown. In this paper, the reactive molecular dynamics simulation technique is used to study the degradation mechanism of pollutants caused by shock-induced nanobubble collapse. We first demonstrate that the propagating shock wave can induce nanobubble collapse, and the collapsing nanobubble has the ability to focus mechanical energy via the converging motion of liquid in the interior of the bubble, leading to the formation of a high-speed jet with a much higher energy density. We also unveil the mechanical nature of long-chain pollutant degradation and the mechanism of free radical generation. Due to the impacting jet, the high-gradient flow has the ability to stretch the long-chain molecule and cause mechanical scission of the molecule in a homolytic manner. Finally, our simulation results reveal that adding ozone molecules to the collapsing bubble would introduce an additional dehydrogenation mechanism.

Hydrogen Peroxide-Assisted Ultrasonic Synthesis of BCNO QDs for Anthrax Biomarker Detection.

A facile 3% hydrogen peroxide-assisted ultrasonic synthetic strategy is demonstrated to successfully synthesize fluorescent boron carbon oxynitride quantum dots (BCNO QDs). The obtained BCNO QDs exhibit intense blue fluorescence and favorable biocompatibility and water solubility. The quantum yield of the BCNO QDs is 19.9%. Owing to the absorbance energy-transfer emission effect, an efficient ratiometric fluorescence biosensor is developed for anthrax biomarker detection based on the BCNO QD-ethylenediaminetetraacetic acid disodium salt-Eu3+ complex. Under optimal conditions, the detection limit of the anthrax biomarker is 0.5 nM. Furthermore, the sensitivity of the system was evaluated by Bacillus subtilis spores and with the detection limit as low as 1.95 × 106 spores. On combining a smartphone with the home-made BCNO QD test paper, the lowest recorded visual detection limit of 1.0 µM anthrax biomarker was achieved using a portable UV lamp. The fast response speed, excellent sensitivity, and selectivity of the approach show potential applications in clinical analysis.