RESUMO
BACKGROUND: Accurate prediction of preterm birth (PTB) is still difficult, mostly because of the multifactorial etiology of PTB. Previous studies have been mostly focused on the prediction of PTB in symptomatic women or those presenting with threatened preterm labor. We aimed to study whether complete blood count (CBC) parameters at 20-30 weeks of pregnancy can predict asymptomatic PTB. METHODS: In this retrospective case-control study, the preterm and term delivery groups were matched by propensity score-matched (PSM) analysis. Baseline data and the CBC parameters examined at 20-30 weeks of gestation were recorded. RESULTS: The combined marker of neutrophil-to-lymphocyte ratio (NLR), hemoglobin (HGB), and platelet distribution width (PDW) accurately predicts PTB at a cutoff value of 0.25, with sensitivity and specificity of 88.6% and 40.5% and negative and positive predictive value of 97.9% and 10.2%, respectively. CONCLUSION: The combined marker of CBC parameters can supplement other markers to predict PTB about 10 weeks in advance. This combined marker had a very high negative predictive value for PTB. Therefore, in subjects with normal combined marker value, further screening tests for PTB may be eliminated unless clinical suspicion is high.
Assuntos
Contagem de Células Sanguíneas , Trabalho de Parto Prematuro , Nascimento Prematuro , Adulto , Biomarcadores/sangue , Estudos de Casos e Controles , Feminino , Humanos , Trabalho de Parto Prematuro/diagnóstico , Trabalho de Parto Prematuro/epidemiologia , Valor Preditivo dos Testes , Gravidez , Nascimento Prematuro/diagnóstico , Nascimento Prematuro/epidemiologia , Pontuação de Propensão , Estudos RetrospectivosRESUMO
PURPOSE: Ovarian cancer is a common gynecological cancer. Herein, we focused on the function and probable mechanisms of LINC00858 in ovarian cancer. METHODS: Real-time quantitative polymerase chain reaction (RT-qPCR) was employed for detecting the expression of LINC00858, miR-134-5p and RAD18 E3 ubiquitin protein ligase (RAD18). Cell proliferation, migration, invasion, epithelial-mesenchymal transition (EMT) and apoptosis were detected by cell counting kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), transwell, terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) and western bolt experiments, as appropriate. Interplays between LINC00858, miR-134-5p and RAD18 were detected by RNA immunoprecipitation (RIP), RNA pull down and luciferase reporter assays. RESULTS: LINC00858 were up-regulated in ovarian cancer tissues and cells, and its expression was elevated in advanced samples compared to early ones. Knocking down LINC00858 inhibited cell proliferation, motility and EMT, but accelerated cell apoptosis in ovarian cancer. Moreover, could be sponged by LINC00858 sponged miR-134-5p to enhance RAD18 expression in ovarian cancer. Also, silenced RAD18 could also restrain oncogenic behaviors of ovarian cancer cells. Rescue experiments showed that overexpressing RAD18 reversed the effects caused by knocking down LINC00858 on cellular processes. CONCLUSION: LINC00858 sequestered miR-134-5p to elevate RAD18 expression, resulting in aggravated development of ovarian cancer. This might provide promising targets for treating patients with ovarian cancer.
Assuntos
Carcinogênese/genética , Carcinoma Epitelial do Ovário/genética , MicroRNAs/metabolismo , Neoplasias Ovarianas/genética , RNA Longo não Codificante/genética , Apoptose , Movimento Celular/genética , Proliferação de Células/genética , Proteínas de Ligação a DNA , Transição Epitelial-Mesenquimal/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , MicroRNAs/genética , RNA Longo não Codificante/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais , Ubiquitina-Proteína Ligases/genética , Ubiquitina-Proteína Ligases/metabolismo , Regulação para CimaRESUMO
BACKGROUND: When a new measurement method is introduced into a clinical laboratory, a comparison study is often needed to ensure that the measurement by the existing method is reproducible by the new method with clinical acceptability. The comparison study of ARCHITECT i2000SR analyzer and i-CHROMATM reader analyzer in measuring plasma human chorionic gonadotropin beta subunit (ß-HCG) has not been reported. METHODS: Blood samples for ß-HCG were collected from pregnant women seen at the outpatient clinic, and they were divided into two groups, those below 20 mU/mL or above, due to its wide concentration range in pregnant women. A comparison study was performed according to EP09-A3 guidelines of the National Clinical and Laboratory Standards Institute (NCCLS). ß-HCG's levels measured from the analyzers being compared were inspected on Bland-Altman plot and outliers were identified by Extreme Studentized Deviate (ESD). Correlation analysis was performed using Passing-Bablok model. RESULTS: Passing-Bablok regression analysis showed that slope B 95% CIs (confidence intervals) of the two groups fall outside of 1, indicating there was a proportional difference between the two methods. Both groups had a ratio of less than 95% percent of the values in the ± 1.96 RSD (residual standard deviation) interval, indicating that there might be inconsistencies between the two methods with respect to random differences. According to Bland-Altman analysis, 95% Limit of Agreement (LOA) between the two methods exceeded the clinically acceptable limits. The deviation between these two detecting platforms was beyond clinically acceptable ranges when samples fell within the concentrations of 15,000 - 30,000 and 1.2 - 20 mU/mL. CONCLUSIONS: Measurements of ß-HCG by ARCHITECT i2000SR and i-CHROMATM Reader are consistent and reproducible only at a certain concentration range. Further research is needed to reduce the biases between these two analyzers.