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Siphon is an effective method to transfer liquid from a higher to a lower level, which has many applications in hygienic design, clinical apparatus, and hydraulic engineering. Traditional operation requires energy to overcome gravity and establish flow in a closed system. Achieving sustainable high flux siphon drainage without energy input remains a challenge due to viscous dissipation. Here, an unexpected open siphon behavior on the South American pitcher plant Heliamphora minor consisting of trichomes covered pitcher and a wedge-shaped sheath is examined. Exploiting the concept of Digital Twin, a new biomimetic research method by transforming the biological sample to a virtual 3D model is proposed and unveiled that maintained connection of wicking on sub-millimeter long trichomes due to asymmetric pressure distribution and ascending in wedge sheath under unbalanced pressure forms continuous surface flow. Exploring this mechanism, a biomimetic siphon device achieving continuous high flux exposed to ambient air is constructed. Besides, particles floating on the meniscus in the outside wedge move under a curvature gradient as water ascends, which implies a biological nutrient capture method and new dust collection manner in the drainage system. Applying the underlying principle enhances the siphon efficiency of floor drains and has the potential for other liquid transfer device design improvements.
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INTRODUCTION: Microvascular perfusion is essential for post-exercise skeletal muscle recovery to ensure adequate delivery of nutrients and growth factors. This study assessed the relationship between various indices of muscle fiber capillarization and microvascular perfusion assessed by contrast-enhanced ultrasound (CEUS) at rest and during recovery from a bout of resistance exercise in older adults. METHODS: Sixteen older adults (72 ± 6 y, 5/11 male/female) participated in an experimental test day during which a muscle biopsy was collected from the vastus lateralis and microvascular perfusion was determined by CEUS at rest and at 10 and 40 min following a bout of resistance exercise. Immunohistochemistry was performed on muscle tissue samples to determine various indices of both mixed and fiber-type-specific muscle fiber capillarization. RESULTS: Microvascular blood volume at t = 10 min was higher compared with rest and t = 40 min (27.2 ± 4.7 vs. 3.9 ± 4.0 and 7.0 ± 4.9 AU, respectively, both p < 0.001). Microvascular blood volume at t = 40 min was higher compared with rest (p < 0.001). No associations were observed between different indices of mixed muscle fiber capillarization and microvascular blood volume at rest and following exercise. A moderate (r = 0.59, p < 0.05) and strong (r = 0.81, p < 0.001) correlation was observed between type II muscle fiber capillary-to-fiber ratio and the microvascular blood volume increase from rest to t = 10 and t = 40 min, respectively. In addition, type II muscle fiber capillary contacts and capillary-to-fiber perimeter exchange index were strongly correlated with the microvascular blood volume increase from rest to t = 40 min (r = 0.66, p < 0.01 and r = 0.64, p < 0.01, respectively). CONCLUSION: Resistance exercise strongly increases microvascular blood volume for at least 40 min after exercise cessation in older adults. This resistance exercise-induced increase in microvascular blood volume is strongly associated with type II muscle fiber capillarization in older adults.
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Fibras Musculares Esqueléticas , Músculo Esquelético , Humanos , Masculino , Feminino , Idoso , Músculo Esquelético/patologia , Ultrassonografia , Perfusão , Exercício Físico/fisiologiaRESUMO
The mechanics of capillary force in biological systems have critical roles in the formation of the intra- and inter-cellular structures, which may mediate the organization, morphogenesis, and homeostasis of biomolecular condensates. Current techniques may not allow direct and precise measurements of the capillary forces at the intra- and inter-cellular scales. By preserving liquid droplets at the liquid-liquid interface, we have discovered and studied ideal models, i.e., interfacial liquids and marbles, for understanding general capillary mechanics that existed in liquid-in-liquid systems, e.g., biomolecular condensates. The unexpectedly long coalescence time of the interfacial liquids revealed that the Stokes equation does not hold as the radius of the liquid bridge approaches zero, evidencing the existence of a third inertially limited viscous regime. Moreover, liquid transport from a liquid droplet to a liquid reservoir can be prohibited by coating the droplet surface with hydrophobic or amphiphilic particles, forming interfacial liquid marbles. Unique characteristics, including high stability, transparency, gas permeability, and self-assembly, are observed for the interfacial liquid marbles. Phase transition and separation induced by the formation of nanostructured materials can be directly observed within the interfacial liquid marbles without the need for surfactants and agitation, making them useful tools to research the interfacial mechanics.
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We derive the ab initio equilibrium statistical mechanics of the gas-liquid-solid contact angle on planar periodic, monodisperse, textured surfaces subject to electrowetting. To that end, we extend an earlier theory that predicts the advance or recession of the contact line amount to distinct first-order phase transitions of the filling state in the ensemble of nearby surface cavities. Upon calculating the individual capacitance of a cavity subject to the influence of its near neighbors, we show how hysteresis, which is manifested by different advancing and receding contact angles, is affected by electrowetting. The analysis reveals nine distinct regimes characterizing contact angle behavior, three of which arise only when a voltage is applied to the conductive liquid drop. As the square voltage is progressively increased, the theory elucidates how the drop occasionally undergoes regime transitions triggering jumps in the contact angle, possibly changing its hysteresis, or saturating it at a value weakly dependent on further voltage growth. To illustrate these phenomena and validate the theory, we confront its predictions with four data sets. A benefit of the theory is that it forsakes trial and error when designing textured surfaces with specific contact angle behavior.
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Dielectrophoresis (DEP) particle separation has label-free, well-controllable, and low-damage merits. Sidewall microelectrodes made of liquid metal alloy (LMA) inherits the additional advantage of thick electrodes to generate impactful DEP force. However, existing LMA electrode-based devices lack the ability to integrate large-array electrodes in a compact footprint, severely limiting flow rate and thus throughput. Herein, a facile and versatile method is proposed to integrate high-density thick LMA electrodes in microfluidic devices, taking advantage of the passive control ability of capillary burst valves (CBVs). CBVs with carefully designed burst pressures are co-designed in microfluidic channels, allowing self-assembly of LMA electrode array through simple hand-push injection. The arrayed electrode configuration brings the accumulative DEP deflection effect. Specifically, The fabricated 5000 pairs of sidewall electrodes in a compact chip are demonstrted to achieve ten times higher throughput in DEP deflection. The 5000-electrode-pair device is applied to successfully separate four mixed samples, including human peripheral blood mononuclear cells and A549 cells with the flow rate of 70 µL min-1. It is envisioned that this work can greatly facilitate LMA electrode array fabrication and offer a robust and versatile platform for DEP separation applications.
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The development of composite photocatalysts with high charge transfer efficiency, great visible light absorption, and quick recovery has aroused the interest of many researchers. Herein, based on the hydrothermal assisted vacuum freeze drying method, CdS, Fe3O4, and N-TiO2 were, respectively, fixed in the inner, middle, and outer layers of nitrogen-doped graphene aerogel for preparation of the site-specific magnetic porous Z-scheme CdS/Fe3O4@N-doped graphene aerogel microtube/N-doped TiO2 (CdS/Fe3O4@NGAM/N-TiO2) photocatalyst. For the composite, Fe3O4@NGAM carrier with porous and tubular structure not only helps the recycle and reactants/productions mass transport in the photocatalytic process but also ensures the well-steered transfer of electrons and holes from CdS and N-TiO2 in the Z-type heterojunction system, greatly improving the separation of photogenerated carriers. Besides, Fe3O4 can also work as a Fenton catalyst to activate hydrogen peroxide which is generated in situ by CdS. Thus, the CdS/Fe3O4@NGAM/N-TiO2 composite presents excellent degradation efficiencies towards methyl orange ((MO) 98% removal rate within 50 min), bisphenol A ((BPA) 96% removal rate within 50 min), tetracycline hydrochloride ((TCH) 96% removal rate within 120 min) and strong stabilities after 6 cycles. The free radical removal experiments show that ·O2- and ·OH are the main active substances of catalysis, which further confirms the synergistic effect of photocatalysis and Fenton catalysis.
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Grafite , Grafite/química , Porosidade , Peróxido de Hidrogênio/química , Catálise , Fenômenos MagnéticosRESUMO
Small-reactor-based polymerase chain reaction (PCR) has attracted considerable attention. A significant number of tiny reactors must be prepared in parallel to capture, amplify, and accurately quantify few target genes in clinically relevant large volume, which, however, requires sophisticated microfabrication and longer sample-to-answer time. Here, single plasmonic cavity membrane is reported that not only enriches and captures few nucleic acids by taking advantage of both capillarity and hydrodynamic trapping but also quickly amplifies them for sensitive plasmonic detection. The plasmonic cavity membrane with few nanoliters in a void volume is fabricated by self-assembling gold nanorods with SiO2 tips. Simulations reveal that hydrodynamic stagnation between the SiO2 tips is mainly responsible for the trapping of the nucleic acid in the membrane. Finally, it is shown that the plasmonic cavity membrane is capable of enriching severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genes up to 20 000-fold within 1 min, amplifying within 3 min, and detecting the trace genes as low as a single copy µL-1. It is anticipated that this work not only expands the utility of PCR but also provides an innovative way of the enrichment and detection of trace biomolecules in a variety of point-of-care testing applications.
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Ouro , Hidrodinâmica , Nanotubos , SARS-CoV-2 , Dióxido de Silício , Ouro/química , SARS-CoV-2/isolamento & purificação , SARS-CoV-2/genética , Dióxido de Silício/química , Nanotubos/química , COVID-19/virologia , COVID-19/diagnóstico , Reação em Cadeia da Polimerase/métodos , Humanos , Limite de DetecçãoRESUMO
Colloidal particles adsorb and remain trapped at immiscible fluid interfaces due to strong interfacial adsorption energy with a contact angle defined by the chemistry of the particle and fluid phases. An undulated contact line may appear due to either particle surface roughness or shape anisotropy, which results in a quadrupolar interfacial deformation and strong long-range capillary interaction between neighboring particles. While each effect has been observed separately, here we report the paradoxical impact of surface roughness on spherical and anisotropic ellipsoidal polymer colloids. Using a seeded emulsion polymerization technique, we synthesize spherical and ellipsoidal particles with controlled roughness magnitudes and topography (convex/concave). Via in situ measurement of the interfacial deformation around colloids at an air-water interface, we find that while surface roughness strengthens the quadrupolar deformation in spheres as expected by theory, in stark contrast, it weakens the same in ellipsoids. As roughness increases, particles of both shapes become more hydrophilic, and their apparent contact angle decreases. Using numerical predictions, we show that this partially explains the decreased interfacial deformation and capillary interactions between the ellipsoids. Therefore, particle surface engineering has the potential to decrease the capillary deformation by asymmetric particles via changing their capillary pinning, as well as wetting behavior at fluid interfaces.
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In the present study, nine fabrics have been tested for brackish water treatment with the aim of industrial application under the concept of zero liquid discharge (ZLD). Moisture content was determined, where it was observed that the lignocellulosic fabrics had a moisture content ranging from 2.5 to 8.5%. The wetting contact angle showed that the flax with polylactic acid (LPLA) was the most hydrophobic. The determination of the liquid absorption capacity showed that, of the synthetic fabrics, the one with the highest absorption, both in distilled water and in brackish water, was the polyester (PES) fabric with an absorption of 816% compared to its initial weight. In the natural fabrics, the highest absorption capacity was shown by the wet-laid without treatment (WL-WT) fabric for both distilled water and brackish water, although it required several cycles of operation to maintain this stable absorption. Exposure to brackish water improved the absorption capacity of all samples. Mechanical and thermal characterization showed that the synthetic fabrics were more resistant than the natural fabrics, although they may compete in terms of applicability. The capillarity study showed that the most hydrophilic fabrics completed the test the fastest. Finally, the composting degradation test showed that those fabrics with polylactic acid (PLA) content degraded faster in the first 14 days and thereafter the degradation of the lignocellulosic content showed a slower degradation until 112 days. The Bam fabric did not degrade during the course of the experiment.
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Sistemas de irrigação mal dimensionados aliados a manejos inadequados podem afetar o uso satisfatório da irrigação em viveiros, ocasionando desperdício de água e energia e, consequentemente, o desenvolvimento inferior das espécies. Sendo assim, os objetivos do trabalho foram avaliar a uniformidade de irrigação de um sistema de microaspersão, já instalado em um viveiro de mudas de espécies florestais, bem como avaliar a uniformidade de um sistema de irrigação por capilaridade de mudas florestais cultivadas em tubetes. A avaliação de uniformidade da irrigação por microaspersão foi realizada em um viveiro de mudas de espécies nativas florestais, em Itutinga MG, utilizando emissores modelos MA-20, Rondo e Inverted Rotor Spray. Para determinação da uniformidade foram calculados o Coeficiente de Uniformidade de Christhiasen (CUC) e o coeficiente de uniformidade de distribuição (CUD) de cada emissor, em três repetições. A uniformidade da irrigação por capilaridade foi avaliada em uma estufa localizada na Universidade Federal de Lavras (UFLA), em três espécies cultivadas em tubetes de 300 cm³ (Araucaria angustifolia (Bertol.) O. Kuntze., Syagrus romanzoffiana e Joannesia princeps) e três espécies cultivadas em tubetes de 110 cm³ (Guazuma ulmifolia Lam., Anadenanthera macrocarpa (Benth.) Brenan e Aspidosperma polyneuron), sendo calculados os coeficientes de uniformidade para cada espécie, também em três repetições. Para os sistemas de microaspersão os maiores valores de CUC e CUD foram obtidos para o emissor MA-20. Na irrigação por capilaridade, os maiores valores de CUC e CUD foram obtidos na espécie Mutamba. Em geral, a uniformidade de irrigação em espécies cultivadas em tubetes de 110 cm³ foi maior que em tubetes de 300 cm³.
Irrigation systems poorly dimensioned allied to inadequate management could affect the satisfactory use of irrigation in nurseries, causing waste of water and energy and, consequently, less species development. Thus, the objectives of this study were to evaluate the uniformity of a microsprinkle irrigation system, already installed in a nursery of forest species, as well as evaluating the uniformity of a capillarity irrigation system of forest seedlings grown in plastic tubes. The microsplinkle irrigation uniformity evaluation was performed in a native forest nursery at Itutinga city, MG, using the emitters models MA-20, Rondo e Inverted Rotor Spray. To determine the irrigation uniformity, were calculated the coefficients CUC (Christiansen Uniformity Coefficient) and DUC (Distribution Uniformity Coefficient) of each emitter, with three replications. The capillary irrigation uniformity evaluation was evaluated in a greenhouse located in the Federal University of Lavras (UFLA), in three species cultivated in 300 cm³ recipient tubes (Araucaria angustifolia (Bertol.) O. Kuntze, Syagrus romanzoffiana e Joannesia princeps) and three species cultivated in 110 cm³ tubes (Guazuma ulmifolia Lam., Anadenanthera macrocarpa (Benth.) Brenan and Aspidosperma polyneuron), through the calculation of CUC and DUC for each species, also with three replications. To the microsprinkle systems, the largest values of CUC and DUC were obtained for the MA-20 emitter. In capillarity irrigation, the largest CUC and DUC were obtained at irrigation of the Guazuma ulmifolia Lam. plants. In general, the irrigation uniformity in the species cultivated in 110 cm³ tubes was higher than in tubes of 300 cm³.