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To date, enumerable fungi have been reported to participate in the biodegradation of several notorious plastic materials following their isolation from soil of plastic-dumping sites, marine water, waste of mulch films, landfills, plant parts and gut of wax moth. The general mechanism begins with formation of hydrophobin and biofilm proceding to secretion of specific plastic degarding enzymes (peroxidase, hydrolase, protease and urease), penetration of three dimensional substrates and mineralization of plastic polymers into harmless products. As a result, several synthetic polymers including polyethylene, polystyrene, polypropylene, polyvinyl chloride, polyurethane and/or bio-degradable plastics have been validated to deteriorate within months through the action of a wide variety of fungal strains predominantly Ascomycota (Alternaria, Aspergillus, Cladosporium, Fusarium, Penicillium spp.). Understanding the potential and mode of operation of these organisms is thus of prime importance inspiring us to furnish an up to date view on all the presently known fungal strains claimed to mitigate the plastic waste problem. Future research henceforth needs to be directed towards metagenomic approach to distinguish polymer degrading microbial diversity followed by bio-augmentation to build fascinating future of waste disposal.
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Plásticos , Poliuretanos , Plásticos/metabolismo , Polímeros , Polietileno/metabolismo , Biodegradação Ambiental , Alternaria/metabolismoRESUMO
While plastics like polyethylene terephthalate can already be degraded efficiently by the activity of hydrolases, other synthetic polymers like polyurethanes (PUs) and polyamides (PAs) largely resist biodegradation. In this study, we solved the first crystal structure of the metagenomic urethanase UMG-SP-1, identified highly flexible loop regions to comprise active site residues, and targeted a total of 20 potential hot spots by site-saturation mutagenesis. Engineering campaigns yielded variants with single mutations, exhibiting almost 3- and 8-fold improved activity against highly stable N-aryl urethane and amide bonds, respectively. Furthermore, we demonstrated the release of the corresponding monomers from a thermoplastic polyester-PU and a PA (nylonâ 6) by the activity of a single, metagenome-derived urethanase after short incubation times. Thereby, we expanded the hydrolysis profile of UMG-SP-1 beyond the reported low-molecular weight carbamates. Together, these findings promise advanced strategies for the bio-based degradation and recycling of plastic materials and waste, aiding efforts to establish a circular economy for synthetic polymers.
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Vanillin, as a promising aromatic aldehyde, possesses worthy structural and bioactive properties useful in the design of novel sustainable polymeric materials. Its versatility and structural similarity to terephthalic acid (TPA) can lead to materials with properties similar to conventional poly(ethylene terephthalate) (PET). In this perspective, a symmetrical dimethylated dialkoxydivanillic diester monomer (DEMV) derived from vanillin was synthesized via a direct-coupling method. Then, a series of poly(ether-ester)s were synthesized via melt-polymerization incorporating mixtures of phenyl/phenyloxy diols (with hydroxyl side-chains in the 1,2-, 1,3- and 1,4-positions) and a cyclic diol, 1,4-cyclohexanedimethanol (CHDM). The polymers obtained had high molecular weights (Mw = 5.3-7.9 × 104 g.mol-1) and polydispersity index (D) values of 1.54-2.88. Thermal analysis showed the polymers are semi-crystalline materials with melting temperatures of 204-240 °C, and tunable glass transition temperatures (Tg) of 98-120 °C. Their 5% decomposition temperature (Td,5%) varied from 430-315 °C, which endows the polymers with a broad processing window, owing to their rigid phenyl rings and trans-CHDM groups. These poly(ether-ester)s displayed remarkable impact strength and satisfactory gas barrier properties, due to the insertion of the cyclic alkyl chain moieties. Ultimately, the synergistic influence of the ester and ether bonds provided better control over the behavior and mechanism of in vitro degradation under passive and enzymatic incubation for 90 days. Regarding the morphology, scanning electron microscopy (SEM) imaging confirmed considerable surface degradation in the polymer matrices of both polymer series, with weight losses reaching up to 35% in enzymatic degradation, which demonstrates the significant influence of ether bonds for biodegradation.
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Materiais Biocompatíveis , Éter , Materiais Biocompatíveis/química , Ésteres , Éteres , Polimerização , Polímeros/químicaRESUMO
In this work, the feasibility of replacing petroleum-based poly(ethylene terephthalate) (PET) with fully bio-based copolyesters derived from dimethyl 2,5-thiophenedicarboxylate (DMTD), dimethyl 2,5-dimethoxyterephthalate (DMDMT), and polysaccharide-derived 1,6-hexanediol (HDO) was investigated. A systematic study of structure-property relationship revealed that the properties of these poly(thiophene-aromatic) copolyesters (PHS(20-90)) can be tailored by varying the ratio of diester monomers in the reaction, whereby an increase in DMTD content noticeably shortened the reaction time in the transesterification step due to its higher reactivity as compared with DMDMT. The copolyesters had weight-average molar masses (Mw) between 27,500 and 38,800 g/mol, and dispersity D of 2.0-2.5. The different polarity and stability of heterocyclic DMTD provided an efficient mean to tailor the crystallization ability of the copolyesters, which in turn affected the thermal and mechanical performance. The glass transition temperature (Tg) could be tuned from 70-100 °C, while the tensile strength was in a range of 23-80 MPa. The obtained results confirmed that the co-monomers were successfully inserted into the copolyester chains. As compared with commercial poly(ethylene terephthalate), the copolyesters displayed not only enhanced susceptibility to hydrolysis, but also appreciable biodegradability by lipases, with weight losses of up to 16% by weight after 28 weeks of incubation.
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Ácidos/química , Poliésteres/síntese química , Polissacarídeos/química , Tiofenos/síntese química , Técnicas de Química Sintética , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Poliésteres/química , Espectroscopia de Infravermelho com Transformada de Fourier , Resistência à Tração , Termogravimetria , Tiofenos/químicaRESUMO
The large-scale usage of petro-chemical-based plastics has proved to be a significant source of environmental pollution due to their non-biodegradable nature. Microbes-based enzymes such as esterases, cutinases, and lipases have shown the ability to degrade synthetic plastic. However, the degradation of plastics by enzymes is primarily limited by the unavailability of a robust enzymatic system, i.e., low activity and stability towards plastic degradation. Recently, the machine learning strategy involved structure-based and deep neural networks show desirable potential to generate functional, active stable, and tolerant polyethylene terephthalate (PET) degrading enzyme (FAST-PETase). FAST-PETase showed the highest PET hydrolytic activity among known enzymes or their variants and degraded broad ranges of plastics. The development of a closed-loop circular economy-based system of plastic degradation to monomers by FAST-PETase followed by the re-polymerization of monomers into clean plastics can be a more sustainable approach. As an alternative to synthetic plastics, diverse microbes can produce polyhydroxyalkanoates, and their degradation by microbes has been well-established. This article discusses recent updates in the enzymatic degradation of plastics for sustainable development.
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Escalated production of plastic, their worldwide distribution and persistent nature finally results into their environmental accumulation causing severe threats to the ecological environment and biotic health. Thus, development of suitable measurements for environmental remediation of plastic may be an urgent issue in this plastic age. Some recent reviews have categorized the microbial species able to degrade different plastic polymers and the different factors effecting bio-degradation of plastic are poorly understood. This review comprehensively discusses bio-degradation of traditional and biodegradable plastic polymers both in natural and biological environment (gut microbes and fungi) to understand different factors regulating their degradation, and also shows how degradation of plastic polymers under abiotic factors influence subsequent biological degradation. Different physicochemical modifications like - breaking large polymers into small fragments by pre-treatment, functional groups enrichment, identifying potent microbial species (consortia) and engineering microbial enzymes might be crucial for bio-degradations of plastic. Effects of micro/nanoplastic and other chemical intermediates, formed during the bio-degradation of plastic, on species composition, abundance, growth, metabolism and enzymatic systems of microbes involved in the bio-degradation of plastic should be determined in future research.
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Plásticos , Polímeros , Biodegradação Ambiental , FungosRESUMO
Halloysite nanotube (HNT) is a natural bio-compatible and stable nanomaterial available in abundance at low-cost. In this work, HNT was modified by two strategies to make it suitable for supporting immobilization of chloroperoxidase (CPO). Firstly, Fe3O4 nanoparticles were deposited on HNT, so magnetic separation can be used instead of centrifugation. Then, the magnetic HNT was modified by 3-aminopropyltriethoxysilane (APTES), which can provide amine group on surface of HNT and meanwhile inhibit the agglomeration of magnetic HNT. Then, HNT-Fe3O4 -APTES was linked with branched polyethyleneimine (PEI) to provide more amino for binding with enzyme. The so-prepared CPO@HNT-Fe3O4-APTES-PEI showed enhanced enzyme loading, reusability, improved thermal stability and tolerance to organic solvents than free CPO. For example, after 10 repeated uses, CPO@HNT- Fe3O4-APTES-PEI can maintain 92.20% of its original activity compared with 65.12% of activity of CPO@HNT-APTES-PEI and 45.69% of activity of CPO@HNT. The kinetic parameters indicated the affinity and specificity of immobilized enzyme to substrate was increased. CPO@HNT-Fe3O4-APTES-PEI was very efficient when it was applied in the degradation of pesticides mesotrione in wastewater. The degradation efficiency can reach 90% within 20 min at range of 5-40 µmol·L-1. These results ensure the potential practical application of this bio-materials in wastewater treatment.
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Ascomicetos/enzimologia , Cloreto Peroxidase/química , Argila/química , Enzimas Imobilizadas/química , Óxido Ferroso-Férrico/química , Proteínas Fúngicas/química , Nanotubos/química , Praguicidas/química , Águas Residuárias/químicaRESUMO
The performance of ethanethiol removal in an anoxic lab-scale bio-scrubber was investigated under different operating parameters and conditions for 300 days. The removal efficiency (RE) of ethanethiol was examined as a function of inlet concentration, empty bed residence time (EBRT) and spray density of irrigation. The results showed the best operation conditions and operation characteristics of the bio-scrubber for this study were at an inlet concentration of 150â¯mg/m3, a spray density of 0.23â¯m3/m2 h and an EBRT of 90â¯s. An average RE of 91% and elimination capacity (EC) of 24.74â¯g/m3 h was found for all inlet ethanethiol concentrations. Variations in spray density higher than 0.23â¯m3/m2 h had no effect on ethanethiol RE at different ethanethiol concentrations. The average experimental yield values were closer to the YET/NO3- theoretical value of 0.74 when the main product was elemental sulphur (So). This indicates that So and other forms of sulphur were formed rather than sulphate (SO42-) as the end product. Furthermore, growth kinetics for bio-degradation were evaluated in batch culture experiments using the Monod model, and bio-kinetic parameters of µmax, Ks, Yxs and qmax were obtained as 0.14 1/h, 1.17â¯mg/L, 0.52 gx/gs and 0.26â¯gs/gx h, respectively.
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Compostos de Sulfidrila , Enxofre , Filtração , CinéticaRESUMO
Microbial remediation of nitroaromatic compounds (NACs) is a promising environmentally friendly and cost-effective approach to the removal of these life-threating agents. Escherichia coli (E. coli) has shown remarkable capability for the biotransformation of 2,4,6-trinitro-toluene (TNT). Efforts to develop E. coli as an efficient TNT degrading biocatalyst will benefit from holistic flux-level description of interactions between multiple TNT transforming pathways operating in the strain. To gain such an insight, we extended the genome-scale constraint-based model of E. coli to account for a curated version of major TNT transformation pathways known or evidently hypothesized to be active in E. coli in present of TNT. Using constraint-based analysis (CBA) methods, we then performed several series of in silico experiments to elucidate the contribution of these pathways individually or in combination to the E. coli TNT transformation capacity. Results of our analyses were validated by replicating several experimentally observed TNT degradation phenotypes in E. coli cultures. We further used the extended model to explore the influence of process parameters, including aeration regime, TNT concentration, cell density, and carbon source on TNT degradation efficiency. We also conducted an in silico metabolic engineering study to design a series of E. coli mutants capable of degrading TNT at higher yield compared with the wild-type strain. Our study, therefore, extends the application of CBA to bioremediation of nitroaromatics and demonstrates the usefulness of this approach to inform bioremediation research.
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Engenharia Metabólica/métodos , Trinitrotolueno/química , Trinitrotolueno/metabolismo , Biodegradação Ambiental , Biotransformação , Catálise , Técnicas de Cultura de Células , Escherichia coli , Cinética , Modelos Biológicos , Fenótipo , Biologia de SistemasRESUMO
This review summarizes the state-of-the-art knowledge of the usage of poly(hydroxy alkanoate)s in medical and sanitary applications. Depending on the monomers incorporated into the polymers and copolymers, this class of polymers exhibits a broad range of (thermo-)plastic properties, enabling their processing by, e.g., solution casting or melt extrusion. In this review, strategies for the polymer analogous modification of these materials and their surfaces are highlighted and correlated with the potential applications of the corresponding materials and blends. While the commercial availability of purified PHAs is addressed in brief, special focus is put on the (bio-)degradability of these polymers and ways to influence the degradation mechanism and/or the duration of degradation.
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Over past decades ionic liquids, a promising alternative to traditional organic solvents, have been dramatically expanding in popularity as a new generation of chemicals with potential uses in various areas in industry. In the literature these compounds have often been referred to as environmentally friendly; however, in recent years the perception of their greenness dramatically changed as the scientific community began to proactively assess the risk of their application based on the entire life-cycle. This review gives a brief overview of the current knowledge regarding the potential risks linked to the application of ionic liquids - from preparation to their disposal, with special emphasis on their potential environmental impacts and future directions in designing inherently safer ionic liquids.
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Meio Ambiente , Poluentes Ambientais/toxicidade , Líquidos Iônicos/toxicidade , Animais , Microbiologia Ambiental , Líquidos Iônicos/química , Testes de ToxicidadeRESUMO
MXene, as a new category of two-dimensional nanomaterials, exhibits a promising prospect in biomedical applications due to its ultrathin structure and morphology, as well as a range of remarkable properties such as biological, chemical, electronic, and optical properties. In this work, different concentrations of MXene (M) were added to polyvinyl alcohol (PVA, P)/nano-hydroxyapatite (n-HA, H) mixed solution, and series of PVA/n-HA/MXene (PHM) composite membranes were obtained by combining sol-gel and freeze-drying processes. Morphology, chemical composition, surface, and mechanical properties of the prepared PHM membranes were characterized by various techniques. Subsequently, the swelling and degradation performances of the composite membranes were tested by swelling and degradation tests. In addition, in vitro studies like cell adhesion, cytotoxicity, proliferation, osteogenic differentiation, and antibacterial properties of MC3T3-E1 were also evaluated. The results showed that the addition of MXene could apparently improve the composite membranes' physicochemical properties, bioactivity, and osteogenic differentiation. Specially, PHM membrane had the best comprehensive properties when the concentration of MXene was set as 2.0% w/v. In a word, the addition of MXene has a positive effect on improving the mechanical properties, osteogenic induction, and antibacterial properties of PH composite membranes, and the prepared PHM composite membranes possess potential applications for guided bone regeneration.
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Materiais Biocompatíveis , Regeneração Óssea , Durapatita , Álcool de Polivinil , Durapatita/química , Durapatita/farmacologia , Álcool de Polivinil/química , Regeneração Óssea/efeitos dos fármacos , Camundongos , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Membranas Artificiais , Fenômenos Mecânicos , Proliferação de Células/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Teste de Materiais , Adesão Celular/efeitos dos fármacos , Antibacterianos/farmacologia , Antibacterianos/química , Regeneração Tecidual Guiada , Nanoestruturas/química , Linhagem Celular , Diferenciação Celular/efeitos dos fármacos , Nanocompostos/químicaRESUMO
The widespread and inevitable use of plastic has led to prospective ecological problems through Bisphenol A (BPA), a synthetic chemical in plastic manufacturing. The present study addresses a unique methodology for eliminating BPA using the assistance of Pseudomonas putida. In the present work, biomass was torrefied to generate biochar with highly porous networks that could accommodate the bacterial species for effective colonization and multiplication. The designed biochar-bacterial globules demonstrated the ability to effectively remove BPA (96.88%) at a concentration of up to 2 g/L. The biochar-bacterial globules could effectively adsorb BPA at a low concentration of 20 mg/L. The alteration in pH did not impact the globule's performance, providing additional support for the practical utilization of these globules in polluted water bodies. In addition, the biochar-bacterial globules exhibited superior effectiveness in degradation compared to the standard levels, particularly in saline conditions. The simplicity and effectiveness of the approach make it promising for real-world implementation in addressing ecological problems associated with BPA contamination.
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Compostos Benzidrílicos , Biodegradação Ambiental , Carvão Vegetal , Fenóis , Pseudomonas putida , Poluentes Químicos da Água , Compostos Benzidrílicos/química , Pseudomonas putida/metabolismo , Carvão Vegetal/química , AdsorçãoRESUMO
The global elimination and restriction of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS), respectively, have urged manufacturers to shift production to their substitutes which still pose threat to the environment with their bioaccumulation, toxicity and migration issues. In this context, efficient technologies and systematic mechanistic studies on the degradation of PFOA/PFOS substitutes are highly desirable. In this review, we summarize the progress in degrading PFOA/PFOS substitutes, including four kinds of mainstream methods. The pros and cons of the present technologies are analyzed, which renders the discussion of future prospects on rational optimizations. Additional discussion is made on the differences in the degradation of various kinds of substitutes, which is compared to the PFOA/PFOS and derives designing principles for more degradable F-containing compounds.
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Ácidos Alcanossulfônicos , Fluorocarbonos , CaprilatosRESUMO
Plastics are widely employed in modern civilization because of their durability, mold ability, and light weight. In the recent decade, micro/nanoplastics research has steadily increased, highlighting its relevance. However, contaminating micro/nanoplastics in marine environments, terrestrial ecosystems, and biological organisms is considered a severe threat to the environmental system. Geographical distribution, migration patterns, etymologies of formation, and ecological ramifications of absorption are just a few topics covered in the scientific literature on environmental issues. Degradable solutions from material science and chemistry are needed to address the micro/nanoplastics problem, primarily to reduce the production of these pollutants and their potential effects. Removing micro/nanoplastics from their discharge points has been a central and effective way to mitigate the adverse pollution effects. In this review, we begin by discussing the hazardous effect on living beings and the identification-characterization of micro/nanoplastics. Then, we provide a summary of the existing degradation strategies, which include bio-degradation and advanced oxidation processes (AOPs), and a detailed discussion of their degradation mechanisms is also represented. Finally, a persuasive summary of the evaluated work and projections for the future of this topic is provided.
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Plásticos , Poluentes Químicos da Água , Plásticos/toxicidade , Microplásticos , Ecossistema , Poluentes Químicos da Água/análise , Poluição AmbientalRESUMO
We isolated two novel bacterial strains, active against the environmental pollutant acetaminophen/Paracetamol®. Streptomyces chrestomyceticus (symbol RS2) and Flavofuscus (symbol M33) collected from El-Natrun Valley, Egypt-water, sediment, and sand samples, taxonomically characterized using a transmission electron microscope (TEM). Genotypic identification, based on 16S rRNA gene sequence analysis followed by BLAST alignment, were deposited on the NCBI as 2 novel strains https://www.ncbi.nlm.nih.gov/nuccore/OM665324 and https://www.ncbi.nlm.nih.gov/nuccore/OM665325 . The phylogenetic tree was constructed. Acetaminophen secondary or intermediate product's chemical structure was identified by GC/LC MS. Some selected acetaminophen secondary-product extracts and derived compounds were examined against a panel of test micro-organisms and fortunately showed a good anti-microbial effect. In silico chemo-informatics Swiss ADMET evaluation was used in the selected bio-degradation extracts for absorption (gastric), distribution (to CNS), metabolism (hepatic), excretion (renal), and finally not toxic, being non-mutagenic/teratogenic or genotoxic, virtually. Moreover, in vitro cytotoxic activity of these selected bio-degradation secondary products was examined against HepG2 and MCF7 cancer cell lines, where M33 and RS2 extract effects on acetaminophen/paracetamol bio-degradation products were safe, with higher IC50 on HepG2 and MCF7 than the acetaminophen/paracetamol IC50 of 108.5 µg/ml. Moreover, an in vivo oral acute single-dose toxicity experiment was conducted, to confirm these in vitro and in silico lower toxicity (better safety) than acetaminophen/paracetamol.
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Polyvinyl alcohol (PVA)-xanthan gum (XG) composite films with good degradation properties were prepared by casting method. The effects of XG amount on thickness, moisture content, water solubility, water vapor transmission (WVP), transmittance and mechanical properties of the composite film were investigated. All composite films produced uniform and transparent films and Fourier transform infrared (FT-IR) spectroscopy, as well as X-ray diffraction (XRD) had proven the formation of hydrogen bonds and subsequently compatibility of the two polymers. In general, addition of XG in PVA was able to decrease moisture content, water solubility and WVP more than the pure PVA films, with sample PX30 demonstrated the best performance. This sample also had the best mechanical properties. It also demonstrated food packaging and capability better than that of commercial plastic bag. More importantly, our sample can be fully decomposed in soil and water within 12 h, which was not only significantly shorter than commercial plastic bag, but also other biodegradable materials. Therefore, PVA/XG-based food packaging material has demonstrated huge potential to be commercialized and replaces commercial plastic bag as an alternative packing material which is renewable, sustainable and environmentally friendly.
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Embalagem de Alimentos , Álcool de Polivinil , Embalagem de Alimentos/métodos , Polissacarídeos Bacterianos/química , Álcool de Polivinil/química , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Textile industries discharges a huge quantity of unused synthetic dyes in wastewater leading to increased environmental pollution and pose a great risk to human health. Thus, a significant improvement in effluent quality is required before it is discharged into the environment. Although, several physicochemical methods have been practiced for the efficient color and dyes removal from textile effluents, these approaches have some drawbacks of greater use of expensive chemicals, low sensitivity, formation of excess sludge which also have secondary disposal problem. Thus, there is still a need for energy efficient, affordable, effective, and environmentally friendly treatment technologies. Bioremediation has been considered as a promising an upcoming active field of research for the treatment of unwanted color and target compounds from the contaminated environment. In order to efficient treatment of textile effluent, the main objective of the present study was to isolate and characterize the indigenous microbial isolates from textile industry effluents and sludge samples and investigate their dye removal and decolorization ability along with the influence of various process parameters on effluents decolorization that draining into the open environment.
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Esgotos , Águas Residuárias , Corantes/toxicidade , Humanos , Indústria Têxtil , Têxteis , Águas Residuárias/químicaRESUMO
Vermicomposting is characterized by transforming organic waste into nutrient-rich organic fertilizer through the action of different earthworms and microorganisms. Although vermicomposting can recycle the excess sludge in an eco-friendly manner, the longer stabilization period has limited its industrial application. The present study sought to investigate a novel operation process of vermicomposting combined with room drying (VD) to improve the stabilization efficiency of dewatered sludge. Subsequently, the performance and efficiency of vermicomposting without room drying, room dry without vermicomposting, and VD for sludge stabilization were compared simultaneously. In the VD process, the sludge water content reduced from 60.8% to 1.64%, showing the highest electrical conductivity and lowest organic matter content, making the humus substances abundant in the final product. Moreover, the vermicomposting achieved the highest ammonia and nitrate content in final product. Additionally, the bacterial and eukaryotic abundances in the VD product were significantly higher (P < 0.01, i.e., 15.6% and 180.7%) than the vermicomposting product. The specific bacterial genus of Glutamicibacter, Chitinibacter, and Acidobacteria was dominated in the VD product. The Partial least squares-Path modeling (PLS-PM) results revealed that the maturity degree in the VD product was significantly associated with microbial component, and the organic form was strongly driven by the change in the physicochemical properties, which was contradictory to vermicomposting model. The study suggests that the VD process could shorten the vermicomposting period by rapidly accelerating the physical, chemical, and biological stabilization of sludge.
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Oligoquetos , Esgotos , Animais , Bactérias , Estudos de Viabilidade , Esgotos/química , SoloRESUMO
Controlling the release of malodorous gas discharged from wastewater treatment plants (WWTPs) has become an urgent environmental problem in recent years. In this study, a bio-trickling filter (BTF) inoculated with microorganisms acclimated to activated sludge in a WWTP was used as the degradation equipment. A continuous degradation experiment with hydrogen sulfide, styrene, and m-xylene in the BTF lasted for 84 days (12 weeks). The degradation capacities of the BTF for hydrogen sulfide, styrene, and m-xylene were evaluated, and the synergy and inhibition among the substrates during biodegradation are discussed. The results indicated that the degradation efficiencies of the BTF were as high as 99.2% for hydrogen sulfide, 94.6% for styrene, and 100.0% for m-xylene. When the empty bed residence time was 30 s, the maximum elimination capacities (EC) achieved for hydrogen sulfide was 38 g m-3 h-1, for styrene was 200 g m-3 h-1, and for m-xylene was 75 g m-3 h-1. Furthermore, the microbial species and quantity of microorganisms in the middle and top of the BTF were much higher than those at the bottom of the BTF. A variety of microorganisms in the BTF can efficiently degrade the typical and highly toxic malodorous gases released from WWTPs. This study can help increase the understanding of the degradation of a mixture of sulfur-containing substances and aromatic hydrocarbons in BTF degradation and promote the development of technologies for the reduction of a complex mixture of malodorous gas emissions from organic wastewater treatment.