Determination of N-acetylcysteine in human plasma by liquid chromatography coupled to tandem mass spectrometry.
J Chromatogr A
; 870(1-2): 13-22, 2000 Feb 18.
Article
in En
| MEDLINE
| ID: mdl-10722057
ABSTRACT
An analytical method for the determination of total N-acetylcysteine in human plasma has been developed, validated and applied to the analysis of samples from a phase I clinical trial. The analytical method consists of plasma digestion with dithiothreitol in order to reduce all the oxidized forms of N-acetylcysteine, and extraction with ethyl acetate followed by determination of levels by an LC-MS-MS method. The intra- and inter-assay precision and accuracy of this technique were good and the limit of quantitation was 50 ng/ml of plasma. The concentration working range was established between 50 ng/ml and 1000 ng/ml. This method has been used in the analysis of approximately 800 human plasma samples from a clinical study with 24 volunteers; the precision of the quality controls was in the range 8.7 to 13.4% and the accuracy was in the range -5.9 to 8.5%, expressed as the RSD and the relative error, respectively.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Acetylcysteine
/
Chromatography, Liquid
Type of study:
Clinical_trials
/
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
J Chromatogr A
Year:
2000
Type:
Article
Affiliation country:
Spain