Simultaneous determination of paracetamol and chlorpheniramine in human plasma by liquid chromatography-tandem mass spectrometry.
J Chromatogr A
; 870(1-2): 77-86, 2000 Feb 18.
Article
in En
| MEDLINE
| ID: mdl-10722064
ABSTRACT
An analytical method for the determination of paracetamol and chlorpheniramine in human plasma has been developed, validated and applied to the analysis of samples from a phase I clinical trial. The analytical method consists in the extraction of paracetamol and chlorpheniramine with diethyl ether, followed by the determination of both drugs by an LC-MS-MS method, using 2-acetamidophenol as internal standard. The intra-assay and inter-assay precision and accuracy of this technique were good and the limit of quantitation was 0.5 microg/ml of plasma for paracetamol and 0.2 ng/ml for chlorpheniramine. The concentration working range was established between 0.5 microg/ml and 25 microg/ml for paracetamol and between 0.2 ng/ml and 50 ng/ml for chlorpheniramine. This method has been used for analyzing more than 1200 human plasma samples from a clinical study with 24 volunteers.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chlorpheniramine
/
Chromatography, Liquid
/
Acetaminophen
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
J Chromatogr A
Year:
2000
Type:
Article
Affiliation country:
Spain