Modification of an automated liquid-handling system for reagent-jet, nanoliter-level dispensing.
Biotechniques
; 30(4): 878-85, 2001 Apr.
Article
in En
| MEDLINE
| ID: mdl-11314270
ABSTRACT
Reducing the scale of biochemical reactions is becoming commonplace. Examples include the screening of large libraries of chemical compounds or gene sequences. These applications demand the ability to transfer sub-microliter volumes of fluid. To this end, we have modified a Hamilton MICROLAB 2200 with high-speed solenoids and a liquid pressurization system to modulate volume delivery down to the nanoliter level. Additional modifications include the use of sapphire-tipped dispensing nozzles and a high-resolution substage to assist in the construction of DNA microarrays. Techniques for characterizing the dispensed volume are presented.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligonucleotide Array Sequence Analysis
/
Microinjections
/
Molecular Biology
Language:
En
Journal:
Biotechniques
Year:
2001
Type:
Article
Affiliation country:
United States