General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin.
Vet Parasitol
; 97(3): 183-9, 2001 Jun 12.
Article
in En
| MEDLINE
| ID: mdl-11390070
ABSTRACT
The variable surface glycoprotein of Trypanosoma evansi RoTat 1.2 variable antigen type (VAT) is used as an antigen in different antibody detection assays for T. evansi. To obtain more information on the predominant character of RoTat 1.2 and its diagnostic potential in antibody detection tests, we checked its expression in 10 different T. evansi stocks and clones from different parts of the world. Cryostabilates were injected into mice and the trypanosomes of the first peak parasitaemia were screened for the presence of RoTat 1.2 by VAT specific immunofluorescence. To monitor the appearance of RoTat 1.2 specific antibodies during infection, rabbits were infected and serologically tested at different time intervals with VAT specific immune trypanolysis, CATT/T. evansi, LATEX/T. evansi and ELISA/T. evansi. Test results confirm the predominant character of RoTat 1.2.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trypanosoma
/
Protozoan Proteins
/
Antigens, Protozoan
/
Antigens, Surface
Limits:
Animals
Country/Region as subject:
America do sul
/
Asia
/
Brasil
Language:
En
Journal:
Vet Parasitol
Year:
2001
Type:
Article
Affiliation country:
Belgium