Long-term effects of osteogenic protein-1 on biosynthesis and proliferation of human articular chondrocytes.
Clin Exp Rheumatol
; 19(5): 525-31, 2001.
Article
in En
| MEDLINE
| ID: mdl-11579711
ABSTRACT
OBJECTIVE:
To study the long-term effects of OP-1 on the biosynthesis and proliferation of human articular chondrocytes.METHODS:
Chondrocytes were released from human cartilage from 10 organ donors of different ages and cultured in alginate. They were exposed to OP-1 (0-200 ng/ml) for 3 to 60 days. Proteoglycan (35S-sulfate) and collagen (3H-proline) synthesis were measured by radiolabeling. Proteoglycan content was determined by a dimethylmethylenblue assay, hydroxyproline content by a colorimetric assay, and DNA content by a fluorometric assay.RESULTS:
Long-term (60 days) cultures of human adult articular chondrocytes stimulated by OP-1 (50 ng/ml) revealed a relative decrease of proteoglycan and collagen synthesis. However, proteoglycan (5-fold) and collagen (1.4-fold) content were increased even after 60 days in culture when compared to controls. Maintaining the chondrocyte phenotype (aggrecan synthesis as the main proteoglycan) in long-term culture, OP-1 (50 ng/ml) stimulated proliferation up to 2.4-fold.CONCLUSION:
Maintaining a stable phenotype and accelerating matrix assembly and proliferation in long-term culture OP-1 might support the tissue engineering of human cartilage.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cartilage, Articular
/
Extracellular Matrix Proteins
/
Transforming Growth Factor beta
/
Bone Morphogenetic Proteins
/
Chondrocytes
Limits:
Adult
/
Aged
/
Child, preschool
/
Humans
/
Infant
/
Middle aged
/
Newborn
Language:
En
Journal:
Clin Exp Rheumatol
Year:
2001
Type:
Article
Affiliation country:
Germany