Mitogenic signaling mediated by oxidants in retinol treated Sertoli cells.
Free Radic Res
; 35(6): 749-55, 2001 Dec.
Article
in En
| MEDLINE
| ID: mdl-11811526
ABSTRACT
Recent intervention studies revealed that supplementation with retinoids resulted in a higher incidence of lung cancer. Recently the causal mechanism has begun to be clarified. We report here that retinol-induced oxidative stress is accompanied by cellular proliferation. Retinol (7 microM) significantly induced thiobarbituric acid reactive species (TBARS) formation, which was inhibited by trolox, superoxide dismutase, N-acetylcysteine and ethanol. This was accompanied by an increase in DNA synthesis and focus formation in cultured rat Sertoli cells. Antioxidants and ethanol inhibited retinol-induced DNA synthesis. Our findings suggest that retinol-induced oxidative stress was associated with cellular proliferation complementing our understanding of the significance of retinol supplementation in neoplastic transformation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sertoli Cells
/
Vitamin A
/
Signal Transduction
/
Oxidants
Limits:
Animals
Language:
En
Journal:
Free Radic Res
Journal subject:
BIOQUIMICA
Year:
2001
Type:
Article
Affiliation country:
Brazil