In situ detection of O6-methylguanine-DNA methyltransferase messenger RNA in paraffin-embedded human astrocytic tumor tissues by nested in situ RT-PCR is useful in predicting chemotherapy-resistance of tumors.

Ohe, Naoyuki; Saio, Masanao; Kijima, Mika; Tamakawa, Noriyuki; Suwa, Tatsuhiko; Kojima, Yasushi; Yano, Hirohito; Kaku, Yasuhiko; Iwama, Toru; Shinoda, Jun; Sakai, Noboru; Takami, Tsuyoshi

Ohe, Naoyuki; Saio, Masanao; Kijima, Mika; Tamakawa, Noriyuki; Suwa, Tatsuhiko; Kojima, Yasushi; Yano, Hirohito; Kaku, Yasuhiko; Iwama, Toru; Shinoda, Jun; Sakai, Noboru; Takami, Tsuyoshi.

Affiliation

Ohe N; Department of Neurosurgery, Gifu University School of Medicine, Gifu 500-8705, Japan.

Int J Oncol ; 22(3): 543-9, 2003 Mar.

Article in En | MEDLINE | ID: mdl-12579307

Subject(s)

Astrocytoma/enzymology; Brain Neoplasms/enzymology; Drug Resistance, Neoplasm; Glioblastoma/enzymology; Neoplasm Proteins/genetics; O(6)-Methylguanine-DNA Methyltransferase/genetics; RNA, Messenger/analysis; RNA, Neoplasm/analysis; Reverse Transcriptase Polymerase Chain Reaction/methods; Astrocytoma/drug therapy; Astrocytoma/pathology; Brain Neoplasms/drug therapy; Brain Neoplasms/pathology; Cell Line, Tumor/drug effects; Cell Line, Tumor/enzymology; Glioblastoma/drug therapy; Glioblastoma/pathology; Humans; Immunoenzyme Techniques; Paraffin Embedding; Sensitivity and Specificity; Specimen Handling

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Collection: 01-internacional Database: MEDLINE Main subject: Astrocytoma / Brain Neoplasms / RNA, Messenger / RNA, Neoplasm / Glioblastoma / Drug Resistance, Neoplasm / O(6)-Methylguanine-DNA Methyltransferase / Reverse Transcriptase Polymerase Chain Reaction / Neoplasm Proteins Type of study: Diagnostic_studies / Evaluation_studies / Prognostic_studies / Risk_factors_studies Limits: Humans Language: En Journal: Int J Oncol Journal subject: NEOPLASIAS Year: 2003 Type: Article Affiliation country: Japan

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