Plant regeneration from callus of apomictic and sexual lines of Paspalum simplex and RFLP analysis of regenerated plants.
Plant Cell Rep
; 21(11): 1040-6, 2003 Jul.
Article
in En
| MEDLINE
| ID: mdl-12835996
ABSTRACT
Culture conditions have been established for the induction of callus from different explants of Paspalum simplex. Fast-growing calli were obtained from hypocotyls and roots excised from 5-day-old seedlings on culture medium containing 2,4-dichlorophenoxyacetic acid and kinetin. Rapid plant regeneration from both apomictic and sexual lines was achieved when the medium was supplemented with alpha-naphthaleneacetic acid and benzylaminopurine. Restriction fragment length polymorphism analysis of the apomixis-controlling region of the regenerated plants showed an absence of restriction site variation for the loci analysed, whereas various degrees of variation were detected for the DNA methylation sites of the same loci.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Regeneration
/
Polymorphism, Restriction Fragment Length
/
Paspalum
Language:
En
Journal:
Plant Cell Rep
Journal subject:
BOTANICA
Year:
2003
Type:
Article
Affiliation country:
Italy