A 17.6 kbp region located upstream of the rabbit WAP gene directs high level expression of a functional human protein variant in transgenic mouse milk.
FEBS Lett
; 305(3): 265-8, 1992 Jul 06.
Article
in En
| MEDLINE
| ID: mdl-1299629
ABSTRACT
We have investigated whether DNA regions present in the rabbit whey acidic protein (WAP) promoter/5' flanking sequence could potentially confer, in vivo, high level expression of reporter genes. Transgenic mice were generated expressing a variant of human alpha 1-antitrypsin, which has inhibitory activity against plasma kallikrein under the control of a 17.6 kbp DNA fragment located upstream of the rabbit WAP gene. Up to 10 mg/ml of active and correctly processed recombinant protein were detected in mouse milk, thus suggesting that the far upstream DNA sequences from the rabbit WAP gene might be useful for engineering efficient protein production in the mammary glands of transgenic animals.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Recombinant Fusion Proteins
/
Regulatory Sequences, Nucleic Acid
/
Alpha 1-Antitrypsin
/
Milk Proteins
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
FEBS Lett
Year:
1992
Type:
Article
Affiliation country:
France