Interactions of actin, myosin, and an actin-binding protein of rabbit pulmonary macrophages. III. Effects of cytochalasin B.
J Cell Biol
; 71(1): 295-303, 1976 Oct.
Article
in En
| MEDLINE
| ID: mdl-135766
ABSTRACT
Low concentrations (greater than or equal to 10(-7) M) of cytochalasin B reversibly inhibit the temperature-dependent gelation of actin by an actin-binding protein. The cytochalasin B concentrations which maximally inhibit actin gel formation are 10-fold lower than the concentrations which maximally impair phagocytosis by intact macrophages. Cytochalasin B also prevents the polymerization of monomeric actin in sucrose extracts of macrophages in the absence but not the presence of 0.1 M CKl. 10(-6) M cytochalasin B dissolves macrophage extract gels and gels comprised of purified actin and actin-binding protein by dissociating actin-binding protein from actin filaments. This concentration of cytochalasin B, however, does not depolymerize the actin filatments.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Actins
/
Myosins
/
Contractile Proteins
/
Cytochalasin B
/
Macrophages
Language:
En
Journal:
J Cell Biol
Year:
1976
Type:
Article