Synthesis and conformational and NMR studies of alpha-D-mannopyranosyl and alpha-D-mannopyranosyl-(1----2)-alpha-D-mannopyranosyl linked to L-serine and L-threonine.
Carbohydr Res
; 230(2): 299-318, 1992 Jun 16.
Article
in En
| MEDLINE
| ID: mdl-1394303
ABSTRACT
alpha-D-Mannopyranosyl and alpha-D-mannopyranosyl-(1----2)-alpha-D- mannopyranosyl linked to L-serine and L-threonine have been synthesised as model substances for the linkage region in certain O-linked glycoproteins. Metropolis Monte Carlo simulations were performed with a modified version of the GESA program, to yield theoretical NOEs and interatomic distances as ensemble-average values, and these were compared with results from steady-state NOE experiments. The NOEs were determined as ensemble-average and as global minimum values. NMR chemical shift differences, obtained for signals of the glycopeptides relative to those of the respective monomers, were interpreted in terms of short inter-residue atomic distances as found within the global minima, and on the basis of averaged distances derived from Monte Carlo simulations.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Serine
/
Threonine
/
Mannosides
Language:
En
Journal:
Carbohydr Res
Year:
1992
Type:
Article
Affiliation country:
Sweden