Characteristics of chitosanases from Aspergillus fumigatus KB-1.
Arch Pharm Res
; 26(12): 1036-41, 2003 Dec.
Article
in En
| MEDLINE
| ID: mdl-14723337
ABSTRACT
Two chitosanases produced by Aspergillus fumigatus KB-1 were purified by ion exchange and size exclusion chromatographies. Molecular weights of chitosanases were 111.23 kDa (chitosanase I) and 23.38 kDa (chitosanase II). The N-terminal amino acid sequence of chitosanase II was determined as follows YNLPNNLKQIYDKHKGKXSXVLAKGFTN. The optimum pH of the chitosanase I and II was 6.5 and 5.5, respectively. The optimum temperatures were 60 degrees C for chitosanase land 70 degrees C for chitosanase II. Hydrolysis products of two chitosanases were analyzed by HPLC and GPC. Chitosanase I hydrolyzed substrate to glucosamine. Chitosanase II produced chitooligosaccharides.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aspergillus fumigatus
/
Chitin
/
Glycoside Hydrolases
Language:
En
Journal:
Arch Pharm Res
Year:
2003
Type:
Article