Sequence analysis of 0.5 Mb of the rabbit germline immunoglobulin heavy chain locus.

Ros, Francesca; Puels, Juergen; Reichenberger, Nicole; van Schooten, Wim; Buelow, Roland; Platzer, Josef

Ros, Francesca; Puels, Juergen; Reichenberger, Nicole; van Schooten, Wim; Buelow, Roland; Platzer, Josef.

Affiliation

Ros F; University of Munich, Institut für Molekulare Tierzucht und Biotechnologie, Feodor-Lynen-Str. 25, 81377 München, Germany.

Gene ; 330: 49-59, 2004 Apr 14.

Article in En | MEDLINE | ID: mdl-15087123

ABSTRACT

ABSTRACT

A bacterial artificial chromosome (BAC) library was created using partially digested rabbit chromosomal DNA. Four BAC clones spanning about 0.5 Mb of the rabbit immunoglobulin (Ig) heavy chain locus were isolated and sequenced. Three of the BAC clones were partially overlapping. Thirty-four V elements, 11 D elements, DQ52, six J elements and the coding regions of Cmicro, Cgamma, C and four Calpha genes were identified and characterized. Additionally, the sequence of a fosmid clone spanning Calpha13 and 30 kb 3'enhancer region was determined. The organization of the locus and the potential function of newly identified functional and structural elements are discussed.

Subject(s)

Immunoglobulin Heavy Chains/genetics; Rabbits/genetics; Amino Acid Sequence; Animals; Base Sequence; Chromosomes, Artificial, Bacterial/genetics; DNA/chemistry; DNA/genetics; Genomic Library; Germ-Line Mutation; Immunoglobulin E/genetics; Male; Molecular Sequence Data; Phylogeny; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid

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Collection: 01-internacional Database: MEDLINE Main subject: Rabbits / Immunoglobulin Heavy Chains Type of study: Prognostic_studies Limits: Animals Language: En Journal: Gene Year: 2004 Type: Article Affiliation country: Germany

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