Oligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display library.

Schoonbroodt, Sonia; Frans, Nicolas; DeSouza, Mark; Eren, Rachel; Priel, Smadar; Brosh, Naama; Ben-Porath, Judith; Zauberman, Arie; Ilan, Ehud; Dagan, Shlomo; Cohen, Edward H; Hoogenboom, Hennie R; Ladner, Robert Charles; Hoet, René M

Schoonbroodt, Sonia; Frans, Nicolas; DeSouza, Mark; Eren, Rachel; Priel, Smadar; Brosh, Naama; Ben-Porath, Judith; Zauberman, Arie; Ilan, Ehud; Dagan, Shlomo; Cohen, Edward H; Hoogenboom, Hennie R; Ladner, Robert Charles; Hoet, René M.

Affiliation

Schoonbroodt S; Dyax s.a., Boulevard du Rectorat 27B Sart Tilman, B-4000 Liege 1, Belgium.

Nucleic Acids Res ; 33(9): e81, 2005 May 19.

Article in En | MEDLINE | ID: mdl-15905471

Subject(s)

Cloning, Molecular/methods; DNA, Complementary; Genes, Immunoglobulin; Oligonucleotides/chemistry; Peptide Library; Adolescent; Adult; Animals; Antibodies, Monoclonal/immunology; Antibody Specificity; Biotechnology/methods; Candida albicans/enzymology; Candida albicans/immunology; Female; Glyceraldehyde-3-Phosphate Dehydrogenases/immunology; Humans; Immunoglobulin Fab Fragments/chemistry; Immunoglobulin Fab Fragments/immunology; Immunoglobulin Fab Fragments/isolation & purification; Immunoglobulin Heavy Chains/chemistry; Immunoglobulin Heavy Chains/genetics; Immunoglobulin Variable Region/genetics; Mice; Mice, Inbred BALB C; Middle Aged; Oligonucleotides/metabolism; Polymerase Chain Reaction; Sequence Analysis, DNA

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligonucleotides / Genes, Immunoglobulin / Cloning, Molecular / DNA, Complementary / Peptide Library Limits: Adolescent / Adult / Animals / Female / Humans / Middle aged Language: En Journal: Nucleic Acids Res Year: 2005 Type: Article Affiliation country: Belgium

Fulltext

XML

PubMed Links

Search on Google