Investigation of the utility of published in vitro intrinsic clearance data for prediction of in vivo clearance.
J Pharmacol Toxicol Methods
; 53(2): 106-16, 2006.
Article
in En
| MEDLINE
| ID: mdl-16188462
ABSTRACT
INTRODUCTION:
This study was conducted to compare and contrast published in vitro intrinsic clearance values reported for compounds from different laboratories and the predictivity of these data to project in vivo clearance.METHODS:
A total of 103 compounds were selected for investigation and an exhaustive literature search was conducted to identify in vitro intrinsic clearance (CL,i) values for comparative purposes. The simple well-stirred model was used to predict in vivo clearance using these in vitro intrinsic clearance values.RESULTS:
Data were available in the literature for <10% of the compounds of interest in rat, dog, monkey, or human S9, as well as <10% for dog or monkey microsomes or hepatocytes. Therefore, this comparative exercise was limited to rat and human microsomes and hepatocytes. Examination of the available CL,i values indicated a substantial (up to 100 s-fold) variation in values reported in the literature; this variability translated into substantial variation in predicted in vivo clearance.DISCUSSION:
The literature paucity and variability described here demonstrate the importance of generating experimentally consistent de novo CL,i data for the purpose of method validation or in vitro-in vivo scaling.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pharmaceutical Preparations
/
Xenobiotics
/
Predictive Value of Tests
/
Drug Evaluation, Preclinical
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
J Pharmacol Toxicol Methods
Journal subject:
FARMACOLOGIA
/
TOXICOLOGIA
Year:
2006
Type:
Article
Affiliation country:
United States