Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS.
J Biosci Bioeng
; 90(6): 688-90, 2000.
Article
in En
| MEDLINE
| ID: mdl-16232935
ABSTRACT
The phosphate (P(i))-binding protein PstS is a member of a family of periplasmic proteins that act as high-affinity receptors for active transport systems in bacteria. PstS protein purified from Pseudomonas aeruginosa was immobilized to N-hydroxysuccinimide-activated Sepharose, packed into a plastic column (5 x 70 mm), and examined for its potential ability to remove P(i) from water. The PstS-Sepharose column completely removed P(i) from 32P-labeled pond water containing about 0.5 microM P(i) (0.015 mg P per liter). More than 90% of 32P-P(i) that was retained in the column could be eluted by washing with low-pH water.
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Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Biosci Bioeng
Journal subject:
ENGENHARIA BIOMEDICA
/
MICROBIOLOGIA
Year:
2000
Type:
Article
Affiliation country:
Japan