Cytoplasmic and nuclear interaction between Rb family proteins and PAI-2: a physiological crosstalk in human corneal and conjunctival epithelial cells.
Cell Death Differ
; 13(9): 1515-22, 2006 Sep.
Article
in En
| MEDLINE
| ID: mdl-16397580
ABSTRACT
Extracellular plasminogen activator inhibitor type-2 (PAI-2) is a potent inhibitor of urokinase-type plasminogen activator (u-PA) and also acts as a multifunctional protein. However, the biological activity of intracellular PAI-2, as well as its intracellular targets, until now remain an enigma. Here, we show that pRb2/p130 and Rb1/p105, but not p107, interact with PAI-2 in both the cytoplasm and nucleus of normal primary human corneal and conjunctival epithelial cells. We provided the first in vivo evidence that a specific fragment of the PAI-2 promoter is bound simultaneously by pRb2/ p130, PAI-2, E2F5, histone deacetylase 1 (HDAC1), DNA methyltransferase 1 (DNMT1), and histone methyltransferase (SUV39H1), in normal primary human corneal epithelial cells, and by pRb2/p130, PAI-2, E2F5, HDAC1, and DNMT1, in normal primary human conjunctiva epithelial cells. Our results strongly indicate a physiological interaction between pRb family members and PAI-2, suggesting the hypothesis that pRb2/p130 and PAI-2 may cooperate in modulating PAI-2 gene expression by chromatin remodeling, in normal corneal and conjunctival cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retinoblastoma Protein
/
Plasminogen Activator Inhibitor 2
/
Conjunctiva
/
Epithelium, Corneal
/
Epithelial Cells
/
Retinoblastoma-Like Protein p107
/
Retinoblastoma-Like Protein p130
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Cell Death Differ
Year:
2006
Type:
Article
Affiliation country:
United States