19F NMR evidence for interactions between the c-AMP binding sites on the c-AMP receptor protein from E. coli.
FEBS Lett
; 283(1): 127-30, 1991 May 20.
Article
in En
| MEDLINE
| ID: mdl-1645291
ABSTRACT
The 19F NMR spectra of 3-fluorotyrosine containing c-AMP receptor protein (CRP) from E. coli have been recorded in the presence of increasing amounts of c-AMP. One of the signals (from Tyr B) shifts upfield by 0.6 ppm in the presence of excess c-AMP and shows both slow and fast exchange behaviour during the titration. This is evidence for interactions between the two c-AMP binding sites on the CRP dimer leading to different dissociation rate constants (less than or equal to 75 s-1; greater than or equal to 350 s-1) for complexes containing one and two c-AMP molecules.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cyclic AMP
/
Cyclic AMP Receptor Protein
/
Escherichia coli
Language:
En
Journal:
FEBS Lett
Year:
1991
Type:
Article
Affiliation country:
United kingdom