Capillary liquid chromatography/atmospheric-pressure matrix-assisted laser desorption/ionisation ion trap mass spectrometry: a comparison with liquid chromatography/matrix-assisted laser desorption/ionisation time-of-flight and liquid chromatography/electrospray ionisation quadrupole time-of-flight for the identification of tryptic peptides.
Rapid Commun Mass Spectrom
; 20(5): 829-36, 2006.
Article
in En
| MEDLINE
| ID: mdl-16470569
ABSTRACT
The atmospheric-pressure matrix-assisted laser desorption/ionisation quadrupole ion trap (AP-MALDI-QIT) analysis of tryptic peptides is reported following capillary liquid chromatographic (LC) separation and direct analysis of a protein digest. Peptide fragments were identified by peptide mass fingerprinting from mass spectrometric data and sequence analysis obtained by tandem mass spectrometry of the principal mass spectral peaks using a data-dependent scanning protocol. These data were compared with those from mass spectrometric analysis using capillary LC/MALDI-time-of-flight (TOF) and capillary LC/electrospray ionisation (ESI)-quadrupole TOF. For all three configurations the resulting data were searched against the MSDB database, using MASCOT and the sequence coverage compared for each technique. Complementary data were obtained using the three techniques.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptide Fragments
/
Chromatography, Liquid
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Spectrometry, Mass, Electrospray Ionization
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Rapid Commun Mass Spectrom
Year:
2006
Type:
Article
Affiliation country:
United kingdom