Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay.

Lugovskaya, N N; Scherbakov, A V; Yakovleva, A S; Tsyvanyuk, M A; Mudrak, N S; Drygin, V V; Borisov, A V

Lugovskaya, N N; Scherbakov, A V; Yakovleva, A S; Tsyvanyuk, M A; Mudrak, N S; Drygin, V V; Borisov, A V.

Affiliation

Lugovskaya NN; FGI Federal Centre for Animal Health (FGI ARRIAH), Vladimir 600901, Russia. nlugovskaya@email.ru

J Virol Methods ; 135(2): 292-6, 2006 Aug.

Article in En | MEDLINE | ID: mdl-16675032

ABSTRACT

ABSTRACT

The recombinant antigen obtained by cloning and expressing two IBV nucleocapsid protein fragments (143-414 aa, 281-414 aa) in Escherichia coli was used for the detection of avian infectious bronchitis virus (IBV) specific antibodies in chicken sera by the indirect ELISA (rNpIBV-ELISA). As a result of testing 1524 serum samples the diagnostic sensitivity and specificity of rNpIBV-ELISA when comparing those of the routine whole IBV ELISA have been shown to be 93.81% and 87.36%, respectively. The agreement value was 91.5%.

Subject(s)

Antibodies, Viral/blood; Enzyme-Linked Immunosorbent Assay/methods; Infectious bronchitis virus/immunology; Nucleocapsid Proteins/immunology; Animals; Chick Embryo; Chickens; Recombinant Proteins/immunology; Vaccination

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Enzyme-Linked Immunosorbent Assay / Infectious bronchitis virus / Nucleocapsid Proteins / Antibodies, Viral Type of study: Diagnostic_studies Limits: Animals Language: En Journal: J Virol Methods Year: 2006 Type: Article Affiliation country: RUSSIA

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