Releasable luciferin-transporter conjugates: tools for the real-time analysis of cellular uptake and release.
J Am Chem Soc
; 128(20): 6526-7, 2006 May 24.
Article
in En
| MEDLINE
| ID: mdl-16704230
ABSTRACT
The design, synthesis, and evaluation of conjugates of arginine-rich transporters and luciferin are described that release luciferin only after entry into cells that are stably transfected with luciferase. Each molecule of free luciferin that is released after entry generates a photon that can be measured allowing for real-time quantification of uptake and release in cells. The process provides a method to assay uptake and release of free luciferin as a function of variations in the releasable linker and in the transporter.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biological Transport
/
Firefly Luciferin
Limits:
Animals
Language:
En
Journal:
J Am Chem Soc
Year:
2006
Type:
Article
Affiliation country:
United States