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Crystallization and preliminary X-ray diffraction analysis of a tRNASer acceptor-stem microhelix.
Förster, Charlotte; Krauss, Norbert; Brauer, Arnd B E; Szkaradkiewicz, Karol; Brode, Svenja; Hennig, Klaus; Fürste, Jens P; Perbandt, Markus; Betzel, Christian; Erdmann, Volker A.
Affiliation
  • Förster C; Institute of Chemistry and Biochemistry, Free University Berlin, Thielallee 63, 14195 Berlin, Germany.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 62(Pt 6): 559-61, 2006 Jun 01.
Article in En | MEDLINE | ID: mdl-16754981
ABSTRACT
In order to understand elongator tRNA(Ser) and suppressor tRNA(Sec) identity elements, the respective acceptor-stem helices have been synthesized and crystallized in order to analyse and compare their structures in detail at high resolution. The synthesis, crystallization and preliminary X-ray diffraction results for a seven-base-pair tRNA(Ser) acceptor-stem helix are presented here. Diffraction data were collected to 1.8 A, applying synchrotron radiation and cryogenic cooling. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 36.14, b = 38.96, c = 30.81 A, beta = 110.69 degrees .
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligonucleotides / RNA, Transfer, Ser Language: En Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Year: 2006 Type: Article Affiliation country: Germany
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligonucleotides / RNA, Transfer, Ser Language: En Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Year: 2006 Type: Article Affiliation country: Germany