RecB-dependent mutator phenotype in Neisseria meningitidis strains naturally defective in mismatch repair.
DNA Repair (Amst)
; 5(12): 1428-38, 2006 Dec 09.
Article
in En
| MEDLINE
| ID: mdl-16911877
ABSTRACT
Several invasive serogroup B meningococcal strains phylogenetically related to the lineage III (ET-24) exhibited a mutator phenotype as shown by mutagenicity assay using rifampicin-resistance as a selection marker. Hypermutation was associated to the presence of defective mutL alleles that were genetically characterized. Interestingly, the mutator phenotype was suppressed when a non-functional recB(ET-37) allele, derived from ET-37 meningococcal strains, replaced the functional recB allele in a lineage III strain. In contrast, the same gene replacement did not affect mutation frequencies in a mismatch repair-proficient strain. These results suggested that in MutL-deficient strains spontaneous mutations mostly arise from post-replicative DNA synthesis associated to the activity of the RecBCD recombination pathway.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adenosine Triphosphatases
/
Exodeoxyribonuclease V
/
DNA Mismatch Repair
/
Mutation
/
Neisseria meningitidis
Limits:
Humans
Language:
En
Journal:
DNA Repair (Amst)
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Year:
2006
Type:
Article
Affiliation country:
Italy