Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria.

Brockmeier, Ulf; Caspers, Michael; Freudl, Roland; Jockwer, Alexander; Noll, Thomas; Eggert, Thorsten

Brockmeier, Ulf; Caspers, Michael; Freudl, Roland; Jockwer, Alexander; Noll, Thomas; Eggert, Thorsten.

Affiliation

Brockmeier U; Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, im Forschungszentrum Jülich, D-52426 Jülich, Germany.

J Mol Biol ; 362(3): 393-402, 2006 Sep 22.

Article in En | MEDLINE | ID: mdl-16930615

Subject(s)

Bacillus subtilis/metabolism; Protein Sorting Signals/physiology; Recombinant Proteins/biosynthesis; Amino Acid Sequence; Bacillus subtilis/genetics; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Base Sequence; Biological Transport, Active; Biotechnology; Carboxylic Ester Hydrolases/biosynthesis; Carboxylic Ester Hydrolases/genetics; DNA, Bacterial/genetics; Escherichia coli/genetics; Esterases/biosynthesis; Esterases/genetics; Fusarium/enzymology; Fusarium/genetics; Gram-Positive Bacteria/genetics; Gram-Positive Bacteria/metabolism; Kinetics; Molecular Sequence Data; Peptide Library; Plasmids/genetics; Protein Processing, Post-Translational; Protein Sorting Signals/genetics; Recombinant Proteins/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Bacillus subtilis / Recombinant Proteins / Protein Sorting Signals Type of study: Diagnostic_studies / Screening_studies Language: En Journal: J Mol Biol Year: 2006 Type: Article Affiliation country: Germany

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